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Preparation method for zinc-doped hydroxyapatite fibroin composite membrane with bone facilitating function

A zinc hydroxyapatite and composite membrane technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as inability to solve the sequelae of matrix metal, achieve high efficiency and safety of bone tissue damage, simple and convenient operation, and repair Effects of Bone Tissue Damage Problems

Inactive Publication Date: 2018-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it still cannot solve the sequelae caused by the corrosion of the base metal in the body

Method used

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  • Preparation method for zinc-doped hydroxyapatite fibroin composite membrane with bone facilitating function
  • Preparation method for zinc-doped hydroxyapatite fibroin composite membrane with bone facilitating function
  • Preparation method for zinc-doped hydroxyapatite fibroin composite membrane with bone facilitating function

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preparation example Construction

[0025] The invention discloses a method for preparing a zinc-doped hydroxyapatite silk fibroin composite membrane with the function of promoting osteogenesis, and is characterized in that the specific preparation steps are as follows:

[0026] 1), prepare a silk fibroin solution with a concentration of 5%-25%, add it dropwise on the titanium plate electrode, and after air-drying under natural conditions, treat it with ethanol to make it β-form, and make an SF / Ti sheet;

[0027] 2), preparing a mixed solution of calcium nitrate and ammonium dihydrogen phosphate as the electrolyte, keeping the calcium-phosphorus ratio in the solution system at 1.67, adding a certain concentration of zinc nitrate, and adjusting the pH value to between 5-8 with NaOH solution;

[0028] 3) An electrochemical three-electrode system is adopted, with the SF / Ti sheet as the cathode and the platinum ring as the anode, adding electrolyte, and performing electrochemical deposition at room temperature.

Embodiment 1

[0031] Prepare a 10% silk fibroin solution, drop it on the titanium plate electrode, air-dry it under natural conditions, and treat it with ethanol to make it β. Prepare a mixed solution of calcium nitrate and ammonium dihydrogen phosphate as the electrolyte, keep the ratio of calcium to phosphorus in the solution system at 1.67, add a certain concentration of zinc nitrate to make the ratio of calcium ion and zinc ion concentration 1:0.1, and use NaOH solution Adjust the pH value to 5, adopt an electrochemical three-electrode system, use the SF / Ti sheet as the cathode, and the platinum ring as the anode, add electrolyte, perform electrochemical deposition at room temperature, and observe its microstructure under SEM as follows: figure 1 , it can be seen that the surface of the prepared composite membrane is evenly distributed with a foam-like structure composed of nano-sized particles, which has a large specific surface area and uniform deposition rules, which is conducive to c...

Embodiment 2

[0036] Prepare a 5% silk fibroin solution, drop it on the titanium plate electrode, air-dry it under natural conditions, and treat it with ethanol to make it β. Prepare a mixed solution of calcium nitrate and ammonium dihydrogen phosphate as the electrolyte, keep the calcium-phosphorus ratio in the solution system at 1.67, add a certain concentration of zinc nitrate so that the ratio of calcium ion and zinc ion concentration is 1:0.5, and use NaOH solution Adjust the pH value to 5.5, adopt an electrochemical three-electrode system, use SF / Ti sheet as cathode, platinum ring as anode, add electrolyte, and carry out electrochemical deposition at room temperature.

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Abstract

The invention discloses a preparation method for a zinc-doped hydroxyapatite fibroin composite membrane with a bone facilitating function. The preparation method comprises the following steps: preparing a fibroin solution with concentration of 5%-25%, dropwise adding to a titanium plate electrode, after airing in natural conditions, processing by using ethyl alcohol, and performing Beta-hydroxylation, preparing an SF / Ti tablet, preparing mixed solution of calcium nitrate and ammonium dihydrogen phosphate as electrolyte, keeping a calcium-phosphorus ratio in a solution system to be 1.67, adding zinc nitrate with a certain concentration, adjusting a pH value to be 5-8 by using a NaOH solution. The preparation method is capable of, through good control of a fibroin membrane to hydroxyapatite, facilitating nucleus formation and growth of the hydroxyapatite in hydroxyapatite mineralized liquid, wherein the obtained hydroxyapatite in various forms expresses different biocompatibility and bone induction activity, bone repair is promoted, and a new idea for developing a fibroin bone tissue repairing material is provided.

Description

technical field [0001] The invention belongs to a processing method of silk fibroin, in particular to a preparation method of a zinc-doped hydroxyapatite silk fibroin composite film with the function of promoting bone formation. Background technique [0002] Hydroxyapatite is the main inorganic component in human bone tissue. It has good biocompatibility, osteoinductivity and osteoconductivity, and has a good application prospect in the field of bone tissue engineering. Studies have shown that the morphology, particle size, and shape of hydroxyapatite have a great influence on its osteogenesis. Hydroxyapatite in natural bone tissue is a needle-like nanoparticle formed by the interaction of calcium ions, phosphate ions and natural collagen in the body. How to use the interaction between organic molecules and inorganic ions to prepare hydroxyapatite morphology with good stem cell differentiation and osteogenic properties is one of the research hotspots in the field of bone ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/44A61L27/56
CPCA61L27/56A61L27/446A61L27/46A61L2430/02C08L89/00
Inventor 杨明英王天云
Owner ZHEJIANG UNIV
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