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A new way to diagnose and monitor oral cancer

A technology for oral cancer and oral leukoplakia, which is applied in the new field of diagnosis and monitoring of oral cancer, and can solve the problems of traumatic and irritating lesions, affecting observation, and limiting inspection repeatability

Active Publication Date: 2021-07-02
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Since the examination itself is traumatic and irritating to the lesion, the postoperative scar may also affect the observation, further limiting the reproducibility of the examination

Method used

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  • A new way to diagnose and monitor oral cancer
  • A new way to diagnose and monitor oral cancer
  • A new way to diagnose and monitor oral cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Changes of important signal pathways during oral cancer before cancer

[0074] method

[0075] Choose clinical and pathological diagnosis as tissue specimens of oral mucosalous whiteprint (simple hyperplasia), white spots with abnormal hyperplasia and leukular cancer (oral squamous cell carcinoma) as the study object. Exemplary H & E Dyeing pathology of the tissue specimen figure 1 Indicated.

[0076] Based on pathological diagnosis results: divided into a white spot simple hyperplasia group (n = 15); white spots with abnormal hyperplasia group (n = 10), cancer group, also known as oral cancer group (n = 15).

[0077] Normal control group (N = 5) tissue specimens were selected from the group consolidation of oral mucosa disease, and some normal tissues needed for surgical treatment and willing to provide patients for research.

[0078] Immunohistochemical detection

[0079] The tissue samples of normal group, white spots, abnormal hyperplasia, and oral cancer gro...

Embodiment 2

[0094] Example 2 Oral saliva extracted body carry miR-185, consistency, reactive oral cancer pre-lesion

[0095] method

[0096] Subject: Patients with oral mucosa in the clinical and pathological diagnosis of the above Example 1, patients with oral mucosa, abnormal hyperplasia, oral cancer (oral squamous cell carcinoma) and normal human oral The following methods were collected, and the oral subordinate bodies were purified.

[0097] The patient or normal people did not have a mouth before taking a varying, and the water was fasted for 1 hour. Sitting in saliva, the head is naturally low, and the saliva in the mouth is naturally spit out of the disposable tray, about 2 ml, do not cough. The collected saliva is immediately placed in a small center tube, stored at 4 ° C.

[0098] The sample 4 ° C, 410,000 × g was centrifuged for 20 minutes, and the supernatant was filtered twice by flash of 0.22 μm, and the ultra-speed centrifuge (Beckman Coulter, Brea, Ca, US) was filtered by a T...

Embodiment 3

[0113] Example 3 Dynamic changes in oral saliva extract in the transformation of oral cancer

[0114] method

[0115] The purified saliva extremum was collected in the above Example 2, quantitative analysis was carried out by the following method.

[0116] Purified saliva extracrystology samples evaluated the size and frequency of extracted in Malverninstruments, WESTBORANOUGH (MA).

[0117] The nanoparticle tracking analysis was performed by using the Nanosight NS300 instrument (Nanosight NTA 2.3 NanopArticle Tracking Andand Analysis Release Version Build 0025). The size distribution of the preparation of the preparation is analyzed by using the Nanosight LM10 system (Nanosight, Wiltshire, United Kingdom) equipped with fast video capture and particle tracking software, and quantitatively analyzes the size of the preparation of the Brown. The purified exosomass was diluted in 400 μl of 1 × PBS / 5 mM EDTA solution, and then the sample was injected into the Nanosight sample chambe...

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Abstract

The invention provides a new method for monitoring the transformation of oral leukoplakia to oral cancer and diagnosing oral cancer, including monitoring the concentration change of oral exosomes in subjects with oral leukoplakia, so as to make a judgment on the pathological state of the disease and the risk of canceration .

Description

Technical field [0001] The present invention relates to a new method of painless and simple monitoring of the transformation of the oral white spot to oral cancer, including monitoring the concentration of the subject's saliva extracry, thereby determining the pathological stage of the oral white spot, and realizes the cancerous changes before cancer High-risk patients, timely treatment of treatment measures, and achieve early and effective prevention and treatment of oral cancer. Background technique [0002] Oral cancer is one of the most common 10 kinds of cancer in the world, 80% of the neck malignant tumors, about 5 million oral cancer patients around the world, in which oral squamous cell carcinoma (Oral Squamous Cell Carcinoma, Oscc) ) More, its five-year survival rate is about 35-57%, about 130,000 cavity cancer patients die every year [1-2] . Oral cancer is mainly in the middle and elderly people. Although progress in diagnosis technology, surgery, chemotherapy, radiothe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57407
Inventor 关晓兵周宇陈莉
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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