Systems, devices, and methods for time-resolved fluorescent spectroscopy
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A spectral band and optical signal technology, applied in fluorescence/phosphorescence, spectrometry/spectrophotometry/monochromator, instruments for radiological diagnosis, etc., can solve a lot of time problems
Inactive Publication Date: 2018-11-23
BLACK LIGHT SURGICAL INC +1
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However, to resolve another wavelength from the emission signal, the sample must be excited again to re-emit the signal, and the scanning monochromator must be retuned to the new wavelength
Since switching between wavelengths can...
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[0193] In the following detailed description, reference is made to the accompanying drawings which form a part hereof. In the drawings, similar symbols typically identify similar components, unless otherwise indicated. The illustrative embodiments described in the detailed description, drawings, and claims are not meant to be limiting. Other embodiments may be utilized, and other changes may be made, without departing from the scope of the subject matter presented herein. It will be readily understood that the various aspects of the disclosure, as generally described herein and illustrated in the drawings, may be arranged, substituted, combined, separated and designed in a wide variety of different configurations, all of which encompass in this article.
[0194] While certain embodiments and examples are disclosed below, the inventive subject matter extends beyond the specifically disclosed embodiments to other alternative embodiments and / or uses, and to modifications and eq...
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Abstract
Provided herein are devices, systems, and methods for characterizing a biological sample in vivo or ex vivo in real-time using time-resolved spectroscopy. A light source generates a light pulse or continuous light wave and excites the biological sample, inducing a responsive fluorescent signal. A demultiplexer splits the signal into spectral bands and a time delay is applied to the spectral bandsso as to capture data with a detector from multiple spectral bands from a single excitation pulse. The biological sample is characterized by analyzing the fluorescence intensity magnitude and/or decayof the spectral bands. The sample may comprise one or more exogenous or endogenous fluorophore. The device may be a two-piece probe with a detachable, disposable distal end. The systems may combine fluorescence spectroscopy with other optical spectroscopy or imaging modalities. The light pulse may be focused at a single focal point or scanned or patterned across an area.
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[0001] cross reference [0002] This application claims U.S. Provisional Application No. 62 / 317,443 filed April 1, 2016, U.S. Provisional Application No. 62 / 317,449 filed April 1, 2016, 62 filed April 1, 2016 U.S. Provisional Application No. 317,451, U.S. Provisional Application No. 62 / 317,452, filed April 1, 2016, U.S. Provisional Application No. 62 / 317,453, filed April 1, 2016, filed April 1, 2016 U.S. Provisional Application No. 62 / 317,455 filed April 1, 2016 U.S. Provisional Application No. 62 / 317,456 filed April 1, 2016 U.S. Provisional Application No. 62 / 317,459 filed April 1, 2016 Benefit of U.S. Provisional Application No. 62 / 317,460, filed April 1, and U.S. Provisional Application No. 62 / 351,615, filed June 17, 2016, which are incorporated herein by reference. Background technique [0003] Laser-induced fluorescence spectroscopy (LIFS) has the ability to reveal qualitative and quantitative information about the chemical or biochemical composition of organic samples....
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