Method for improving animal semen quality by use of chestnut flower ethyl acetate extract

A technology of ethyl acetate extraction and ethyl acetate, applied in the field of chestnut flower ethyl acetate extract to improve the quality of animal semen, to achieve the effects of promoting spermatogenesis, expanding the scope of use, and improving the quality of semen

Active Publication Date: 2018-11-30
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that there are many active ingredients in chestnut flowers, which have anti-oxidation, anti-aging, anti-inflammatory and other effects. However, the ingredients in chestnut flower extracts obtained by different extraction methods are also different. Do the active ingredients in chestnut flowers also have the effect of improving semen? The efficacy of quality is unknown and there is no literature disclosing the use of chestnut flower ethyl acetate extract to improve semen quality

Method used

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  • Method for improving animal semen quality by use of chestnut flower ethyl acetate extract
  • Method for improving animal semen quality by use of chestnut flower ethyl acetate extract
  • Method for improving animal semen quality by use of chestnut flower ethyl acetate extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: preparation chestnut flower ethyl acetate extract

[0029] 1) Pick fresh chestnut male flowers in full flowering stage and immediately place them in ethyl acetate with a solid-to-liquid ratio of 45g / L. Use a homogenizer to fully homogenize the chestnut flowers, and then ultrasonically extract them in an ultrasonic instrument with a power of 820W at 30°C for 2 hours. Subsequently, it was centrifuged at 10000r / min for 60min, the ethyl acetate layer solution was collected, and the obtained ethyl acetate layer solution was rotary evaporated and freeze-dried to obtain a yellow powder;

[0030] 2) Wash the yellow powder obtained in step 1) 3 times with 50% alcohol, then freeze-dry, dissolve the dried powder in ethyl acetate again, and then use suction filtration with an ultra-microfiltration membrane with a pore size of 0.45 μm. The ethyl acetate solution obtained by filtering is freeze-dried again to obtain the solid chestnut flower ethyl acetate extract;

[0...

Embodiment 2

[0032] Embodiment 2: Mouse feeding test

[0033] 240 3-week-old mice (with uniform body weight) of the healthy ICR strain were randomly assigned to 8 groups (30 / group), and the mice were eating and drinking normally, and were raised in a unified SPF rat room . Take by weighing a certain amount of embodiment 1 step 3) gained Chinese chestnut flower ethyl acetate extract powder every day according to table 1 design, be dissolved in DMSO, be mixed with the mother liquor of high concentration, utilize physiological saline to dilute to required concentration then, finally DMSO<0.5%. Each mouse was given an ethyl acetate extract solution of chestnut flower matching its body weight by intragastric administration on time every day. Each mouse was gavaged with 0.1 mL ethyl acetate extract solution of chestnut flower. After 5 weeks of gavage, the semen of the mice was collected, and the sperm motility and density were analyzed with a semen quality analyzer. The test results are show...

Embodiment 3

[0037] Embodiment 3: Pig feeding test

[0038]In the cooperative experimental base of our school, 70 healthy weaned (35-day-old) boars were selected and randomly divided into 7 groups (10 heads / group). All pigs are in a large breeding room, eating and drinking normally, and are under unified management. Take a certain amount of chestnut flower ethyl acetate extract powder obtained in step 3) of embodiment 1 every day according to the design of Table 2, and fully mix the weighed animal concentrate and chestnut flower ethyl acetate extract powder. Feed the prepared feed concentrate containing chestnut flower ethyl acetate extract to pigs on time every day. Feed until 6 months old, collect the semen of the pigs, and use the semen quality analyzer to analyze the sperm motility and density.

[0039] Table 2 Effects of swine supplementation with chestnut flower ethyl acetate extract on semen quality

[0040]

[0041] Experiments have found that there is a dose dependence in th...

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Abstract

The invention relates to a method for improving animal semen quality by the use of a chestnut flower ethyl acetate extract. The male ablactation mammals are daily supplemented with the chestnut flowerethyl acetate extract through a gavage mode or a mode of adding into a feed: acceptable daily intake of small male mammals is 0.01-5 mg / kg (weight), and acceptable daily intake of middle-sized male mammals is 0.1-10 mg / kg (feed). The chestnut flower ethyl acetate extract is a freeze-dried powder after ultrasonic extraction and further hyperfiltration. After ultrasonic extraction of the chestnut flower ethyl acetate extract and further hyperfiltration, male mammals are supplemented with the chestnut flower ethyl acetate extract so as to remarkably improve animal semen quality, and the supplementary feeding time starts after ablactation of the male mammals. The technical scheme helps male mammals to better establish a reproductive system, fully tap genetic potential of male animals and broaden using range of the chestnut flower ethyl acetate extract.

Description

technical field [0001] The invention relates to the field of animal reproductive physiology and reproductive technology, in particular to a method for improving the quality of animal semen by using the ethyl acetate extract of chestnut flowers. Background technique [0002] In recent years, due to the excessive or incorrect application of pesticides and fertilizers, environmental pollution, climate change and other reasons, the sperm motility of male animals has decreased, the concentration of sperm has decreased, and the rate of sperm deformity has increased, resulting in a substantial increase in the rate of male infertility. This is a worldwide problem that has attracted widespread attention from all over the world. However, there is no good method to improve semen quality to treat male infertility. Therefore, scientists are looking for effective drugs or methods to improve the semen quality of male animals. [0003] Chestnut (Castanea mollissima BL.), also known as: ch...

Claims

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Application Information

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IPC IPC(8): A61K36/49A61P15/08A61P5/26A61P39/06A61K133/00
CPCA61K36/49A61K2236/33A61K2236/39A61K2236/51A61K2236/53A61P5/26A61P15/08A61P39/06
Inventor 李兰沈伟孙中义赵勇于帅
Owner QINGDAO AGRI UNIV
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