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Streptomyces halotolerant, its agent and its application in promoting plant growth

A technology of Streptomyces vulgaris and Streptomyces olivine is applied in the application field of promoting plant growth to achieve the effects of stable activity, simple use and low cost

Active Publication Date: 2021-06-18
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently the most studied plant growth-promoting bacteria are fungal resources such as bacteria and mycorrhizal fungi, but there are few reports on the improvement of crop growth under salt stress by actinomycetes and related bacterial agents.

Method used

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  • Streptomyces halotolerant, its agent and its application in promoting plant growth
  • Streptomyces halotolerant, its agent and its application in promoting plant growth
  • Streptomyces halotolerant, its agent and its application in promoting plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Screening process

[0027] 1. Collect halophyte leaf samples from the saline soil in the coastal zone of Lianyungang City, Jiangsu Province, homogenize and crush the surface-sterilized plant leaf samples in a sterile mortar, and perform gradient dilution with sterile water. Take 10 -2 50 microliters of the diluted homogenate were spread on the separation medium (yeast extract 0.25g, K 2 HPO 4 0.5g, water 1L, pH 7.2).

[0028] 2. After the plate is coated, culture it in a constant temperature incubator at 28°C. Check the colony growth on the plate every 24 hours. After 3-7 days, according to the different characteristics of the colony shape, size, color, etc., select different isolated strains and store them in the ISP 2 solid slope. Pure strains were inoculated into ISP 2 liquid medium, and different concentrations of NaCl (0-15%, interval 1%) were added to the medium, placed on a constant temperature shaker at 28°C, and shake flask culture was carried o...

Embodiment 2

[0029] Example 2: Identification of bacterial strain PE5084

[0030] 1. Observation of culture characteristics: The morphological characteristics of PE5084 cells (hyphae in the base, aerial hyphae, spore filaments, spores, etc.) were observed using the embedded method. The purified PE5084 strain was inoculated on the ISP 2 solid medium, and four-section line was used to grow at 28°C for 7-14 days, and the growth of the strain and the shape of the colony were observed.

[0031] 2. Observation of microscopic morphological characteristics: On the ISP 2 solid medium, dig out a rectangular groove with a size of about 1cm×3cm, smear PE5084 with a bamboo stick around the groove, and place the sterile cover glass on the surface with tweezers. On the groove of the culture medium, after culturing at 28°C for 14 days, the coverslip was taken out with tweezers, glutaraldehyde was added dropwise to the place where the hyphae were on the coverslip, and dried naturally. Observe the imprinte...

Embodiment 3

[0036] Embodiment 3: Detection of growth-promoting performance of bacterial strain PE5084

[0037] Detection of the ability to dissolve inorganic phosphorus: pick a small amount of bacteria and plant them in inorganic phosphorus medium containing calcium phosphate, and cultivate them in a 28°C incubator for 3 to 5 days. Observe whether a transparent circle appears around the colony. If so, it proves that The strain has phosphorus-solubilizing activity. Qualitative detection found that strain PE5084 could dissolve inorganic phosphorus.

[0038] Detection of siderophore activity: Inoculate the strain PE5084 on the CAS detection medium, culture it at 28°C for 5-7 days, observe whether there is a yellow transparent circle around the colony, if it appears, it proves that the strain has siderophore activity, That is positive. Qualitative detection found that strain PE5084 could produce siderophore.

[0039] Detection of plant growth hormone indole acetic acid (IAA): Prepare a 2.5...

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Abstract

Streptomyces olivaceus strain GDMCC No.60272. A microbial inoculant prepared from the above strains. The preparation method of the above-mentioned microbial bacterial agent and the application of the prepared microbial agent in promoting the growth of plants in saline soil environment. The streptomyces strain of the invention has high activity and stability, and can effectively promote the growth of plants in saline soil environment. The microbial agent of the invention has simple preparation method, low production cost, no pollution and is suitable for large-scale application. Irrigation of plants with the microbial agent of the present invention can promote the growth of plants in the saline soil environment, thereby improving the saline soil, with low cost, simple use, no pollution, no toxin residue, and no harm to humans, animals and the environment.

Description

technical field [0001] The present invention relates to microorganisms, microbial inoculum, preparation and application thereof, in particular to a strain of Streptomyces olivaceus (Streptomyces olivaceus) strain GDMCC No.60272, microbial inoculum prepared by the strain, its preparation method and Application of the prepared bacterial agent in promoting plant growth. Background technique [0002] Soil salinization is one of the main factors affecting agricultural production globally. In recent years, due to the influence of human activities and natural factors, the problem of soil salinization has become increasingly serious. According to incomplete statistics, the global salinized soil area is about 8.3×10 9 hm 2 , and our country also has about 6.67×10 6 hm 2 The salinized arable land accounts for about 25% of the arable land area, and it tends to increase year by year. Soil salinization mainly refers to the phenomenon or process of soluble salt accumulation on the so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P21/00C12R1/58
CPCA01N63/00C12N1/20C12N1/205C12R2001/58
Inventor 秦盛龚园熊有威邢云朱雪邢珂徐文秀
Owner XUZHOU NORMAL UNIVERSITY
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