Dengue virus serum type 3 epidemic strain GZ14D3 replicon and application thereof

A technology of dengue virus and replicons, applied in the field of genetic engineering
CN113604483APending Publication Date: 2021-11-05SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2021-11-05

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Abstract

The invention relates to the field of gene engineering, in particular to a dengue virus serum type 3 epidemic strain GZ14D3 replicon and application thereof. A direct-early promoter of cytomegalovirus which can be transcribed in eukaryotic cells is utilized to construct a subgenome replicon of a dengue virus serum type 3 clinical isolate with a luciferase reporter gene, and the subgenome replicon comprises 5 'UTR of the virus, a partial capsid protein sequence and an NS1-NS5 sequence. The replicon plasmid is transfected to a BHK-21 cell, and the BHK-21 cell is screened by using neomycin, so that a DENV-3 monoclonal replicon cell containing a stable expression luciferase reporter gene signal is screened out. The known DENV inhibitor mycophenolic acid is used for verifying that the medicine shows a good virus replication inhibition effect on replicon cells, and the medicine of methylclocycline sulfosalicylate with an inhibition effect on DENV-3 replicon is found.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a dengue virus serotype 3 epidemic strain GZ14D3 replicon and application thereof. Background technique

[0002] A series of diseases caused by dengue virus (DENV) infection are important mosquito-borne viral diseases in tropical areas at present, and the vectors are Aedes aegypti and Aedes albopictus. Before 1970, 9 countries had serious dengue fever epidemics, and dengue has affected more than 100 countries in tropical and subtropical regions at present, and about 390 million dengue fever infection cases occur every year, and about 70% of the cases occur in Asia. The World Health Organization estimates a 30-fold increase in the observed global incidence over the past 50 years. Today, dengue virus poses a major threat to public health worldwide, with approximately two-fifths of the world's population at risk of dengue infection.

[0003] Globally, there are four serotypes of...

Claims

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