Sorting method of SERS based on immunomagnetic bead labeling

Abstract

The present invention proposes a SERS sorting method based on immunomagnetic bead labeling, which includes: S1. On surface receptors unique to each target cell, immunomagnetic beads are labeled and modified with antibodies paired with the surface receptors; S2 .Screen out the target cells; S3. According to the surface receptors unique to each target cell, label the SERS-TAG modified with the antibody paired with the surface receptor on each target cell; S4. After labeling, Each target cell undergoes surface-enhanced Raman detection to determine its type, and the target cells are classified according to their type. The invention can complete multiple CTC detections in one detection, and can greatly reduce the number of surface-enhanced Raman detections and the amount of SERS-TAG, reduce optical detection time, and save costs.

Description

technical field [0001] The invention belongs to the cross technical field of cell sorting and optical engineering, and in particular relates to a SERS sorting method based on immunomagnetic bead labeling. Background technique [0002] Immunomagnetic beads have been widely used in molecular and cell biology, including nucleic acid extraction, sorting and enrichment of specific proteins and cells, etc. Taking immunomagnetic bead-based cell sorting as an example, the process is divided into four steps: (1) surface modification of magnetic beads with specific ligands or antibodies; (2) passing the modified magnetic beads through antigen- The antibody reaction or the ligand-receptor binding reaction binds to the target cells in the sample; (3) magnetically adsorbs the magnetic bead-cell conjugate; (4) removes other cells by washing and other means. [0003] The existing cell screening technology based on immunomagnetic beads directly attracts magnetic beads through magnetic forc...

AI Technical Summary

Problems solved by technology

This protocol can remove other cells and unbound magnetic beads, but it is difficult to ensure that trace target cells are successfully captured

Moreover, this solution uses a chemical combination method, which is difficult to ensure the successful capture of very small cells such as circulating tumor cells, which will cause misdiagnosis in diagnosis

In addition, this method of capturing target cells through antibody-antigen binding requires manual participation and must be completed by professional technicians, which is not conducive to integration and cannot meet the informatization needs of the big data era

[0006] In addition, immunomagnetic beads are used to mark specific antigens or receptors on target cells. This method can only sort out one type of target cells under certain circumstances, and it is impossible to confirm whether other types of tumor cells are contained in whole blood samples.

However, in practical applications, for example, when a whole blood sample is used for tumor cell detection, only one kind of tumor cell is isolated, which is not enough to confirm whether the whole blood sample also contains other types of tumor cells, and cannot be further used for the detection of tumor cells (such as DNA sequencing, etc.) to provide accurate test sample information

At the same time, the same antigen or receptor may also exist on different tumor cells, and a variety of tumor cells can be sorted out at one time after being labeled with immunomagnetic beads. Accurate detection of sample information, thereby saving subsequent detection cycles and costs

However, the direct use of SERS detection requires the use of a large number of SERS-TAGs for labeling, resulting in waste, and the time to pass through the optical system is very long and the efficiency is extremely low

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