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Application of eurycomalactone in antifeedant activity and inhibition of insect growth and development

A growth and insect technology, applied in the fields of application, plant growth regulators, chemicals for biological control, etc., can solve the problems of increased resistance of pests, decreased control effect, etc., and achieve high antifeedant activity and good transport Sexuality, effect of good plant systemic properties

Active Publication Date: 2021-01-05
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current control mainly relies on chemical pesticides, but long-term use will increase the resistance of pests and reduce the control effect

Method used

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  • Application of eurycomalactone in antifeedant activity and inhibition of insect growth and development
  • Application of eurycomalactone in antifeedant activity and inhibition of insect growth and development
  • Application of eurycomalactone in antifeedant activity and inhibition of insect growth and development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Eurycomalactone can be sucked into the root through the root of Chinese cabbage, and transported upward to the leaves, so that insects refuse to eat the leaves of Chinese cabbage

[0026] Method: Eurycomalactone was dissolved in DMSO to make a mother solution of 10000μg / mL, and then 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated untreated Chinese cabbage seedlings (6-leaf stage) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it into the above-mentioned 100 μg / mL Eurycomalactone culture solution prepared above, after 24 hours of cultivation, take 0.7 g of the top leaves of Chinese cabbage, mash it and dissolve it in 2 mL of methanol, and use liquid chromatography-mass spectrometry The combined instrument can detect the Eurycomalactone contained in this extract. After calculation, the content of Eurycomalactone in the top leaves (fresh leaves) of...

Embodiment 2

[0032] Embodiment 2: Eurycomalactone inhibits cell proliferation of diamondback moth, Spodoptera litura

[0033] To explore whether Eurycomalactone has an inhibitory effect on the proliferation of diamondback moth embryo cells and Spodoptera litura ovarian cells, the CCK-8 method was used to detect the inhibition rate of cell proliferation. The results are shown in Table 1:

[0034] Table 1 The inhibition rate of Eurycomalactone on the proliferation of insect cells

[0035]

[0036] Experimental conclusion: It can be known from Table 1 that Eurycomalactone has a significant inhibitory effect on the proliferation of insect cells, which is positively correlated with the concentration of Eurycomalactone. The inhibition rate of different concentrations of Eurycomalactone to Plutella xylostella ovary cells is greater than 61%; Eurycomalactone also has a strong inhibitory effect on Plutella xylostella embryo cells; Eurycomalactone is lower than the sensitivity of Plutella xyloste...

Embodiment 3

[0037] Example 3: Antifeedant activity of Eurycomalactone to insects

[0038] Method: Eurycomalactone was dissolved in acetone to make a mother solution of 2000 μg / mL, and then the mother solution was diluted with acetone to 5, 10 and 20 μg / mL, and evenly spread on the surface of a circular cabbage leaf dish with a diameter of 1.5 cm. The amount of drug applied to each leaf dish was 20 μL. After the acetone on the surface of the leaf dish was naturally dried, the 3rd instar larvae of the diamondback moth Plutellaxylostella (Linnaeus) were inserted. Each concentration treatment was repeated 3 times at a relative humidity of 50% to 70%. After keeping in an environment with a temperature of 25° C. to 28° C. for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated. See Example 1 for the calculation formula.

[0039] Cassava leaves were used to test the antifeedant activity of Eurycomalactone against 1st instar larvae of Spodoptera litura (F...

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Abstract

The invention discloses an application of Eurycomalactone with antifeedant activity to insects in restraining growth and development of insects. Eurycomalactone has excellent plant systematic featureand conduction property, inhibiting effect to multiplication of insect cells, antifeedant activity to insects and capability of restraining growth and development of insects. Eurycomalactone is separated and purified from natural plants, is low-toxic, no-residual, non-pollution and pollution-free to commercial crops, such as various cereals, melons, fruits and vegetables, and is more beneficial toenvironmental protection. Eurycomalactone has high antifeedant activity to insects and is capable of achieving a preventing and controlling effect.

Description

technical field [0001] The invention belongs to the field of biological pesticides and plant protection, and in particular relates to the application of Eurycomalactone in the antifeedant activity of insects and the inhibition of growth and development of insects. Background technique [0002] Agriculture is affected by pests and diseases and climatic conditions all year round, and most areas suffer heavy losses every year. Insects headed by Lepidoptera are more serious. Because of their large food intake and strong reproductive ability, larvae gnaw on leaves and excrete pollution, which can cause huge economic losses. [0003] The current control mainly relies on chemical pesticides, but long-term use will increase the resistance of pests and reduce the control effect. Therefore, it is particularly important to explore efficient, safe and environment-friendly green pesticides. The development of botanical pesticides has always been a research hotspot in the field of pesti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/08A01P15/00A01P7/04
CPCA01N43/08
Inventor 匡石滋杨伟群邵雪花赖多肖维强凡超
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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