Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of eurycomanone in insect antifeedant activity and inhibition of insect growth and development

A technology of growth and development and insects, applied in the direction of application, plant growth regulators, insecticides, etc., can solve the problems of difficult hazards, increased resistance of agricultural pests, etc., and achieve environmental protection, good plant systemic characteristics, good transductive effect

Active Publication Date: 2021-01-12
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the long-term and irrational use of chemical agents in large quantities, the resistance of these agricultural pests has been continuously enhanced, so that it is difficult to control their damage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of eurycomanone in insect antifeedant activity and inhibition of insect growth and development
  • Application of eurycomanone in insect antifeedant activity and inhibition of insect growth and development
  • Application of eurycomanone in insect antifeedant activity and inhibition of insect growth and development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Eurycomanone can be sucked into the root through the root of Chinese cabbage, and transported upward to the leaves, so that insects refuse to eat the leaves of Chinese cabbage

[0025] Method: Eurycomanone was dissolved in DMSO to make a mother solution of 10000μg / mL, and then 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated untreated Chinese cabbage seedlings (6-leaf stage) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it into the above-mentioned 100 μg / mL Eurycomanone culture solution prepared above, after 24 hours of cultivation, take 0.5 g of the top leaf of Chinese cabbage, mash it and dissolve it in 2 mL of methanol, and use liquid chromatography-mass spectrometry The combined instrument can detect Eurycomanone contained in this extract. After calculation, the content of Eurycomanone in the top leaves (fresh leaves) of Chinese cabbage reac...

Embodiment 2

[0031] Embodiment 2: Eurycomanone inhibits cell proliferation of diamondback moth, Spodoptera litura

[0032] To explore whether Eurycomanone has an inhibitory effect on the proliferation of diamondback moth embryo cells and Spodoptera litura ovarian cells, the CCK-8 method was used to detect the inhibition rate of cell proliferation. The results are shown in Table 1:

[0033] Table 1 Eurycomanone inhibits the proliferation of insect cells

[0034]

[0035] Experimental conclusion: It can be known from Table 1 that Eurycomanone has a significant inhibitory effect on the proliferation of insect cells, which is positively correlated with the concentration of Eurycomanone. Different concentrations of Eurycomanone have a strong inhibitory effect on the ovary cells of Spodoptera litura and embryonic cells of Plutella xylostella, and there is little difference between them. Under the condition of the experimental concentration greater than 6.25μg / mL, the inhibition rate of Euryc...

Embodiment 3

[0036] Example 3: Antifeedant activity of Eurycomanone to insects

[0037] Method: Eurycomanone was dissolved in acetone to prepare a 2000 μg / mL mother solution, and then the mother solution was diluted with acetone to 5, 10 and 20 μg / mL solutions, and evenly spread on the surface of a circular cabbage leaf dish with a diameter of 1.5 cm. The amount of drug applied to each leaf dish was 20 μL. After the acetone on the surface of the leaf dish was naturally dried, the 3rd instar larvae of the diamondback moth Plutellaxylostella (Linnaeus) were inserted. Each concentration treatment was repeated 3 times at a relative humidity of 50% to 70%. After keeping in an environment with a temperature of 25° C. to 28° C. for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated. See Example 1 for the calculation formula.

[0038] Cassava leaves were used to test the antifeeding activity of Eurycomanone against the 1st instar larvae of Spodoptera litu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of Eurycomanone in antifeedant activity of insects and inhibition of growth and development of insects. The Eurycomanone has good internal absorption characteristics and conductivity, has an inhibitory effect on proliferation of insect cells, and has antifeedant activity on insects and inhibitory activity on growth and development of insects. The Eurycomanone isseparated and purified from natural plants, has low toxicity, no residue, no pollution and no harm for various economic crops such as cereals, melons, fruits and vegetables, and is more favorable forenvironmental protection. The Eurycomanone has high antifeedant activity on insects and can achieve controlling effects.

Description

technical field [0001] The invention belongs to the field of biological pesticides and plant protection, and in particular relates to the application of Eurycomanone in insect antifeedant activity and inhibition of insect growth and development. Background technique [0002] Plutella xylostella, beet armyworm, Spodoptera litura and aphids are all omnivorous agricultural pests widely distributed in the world, which are very harmful to agriculture, pollute land, air and water resources and endanger human health. At present, due to the long-term and irrational use of chemical agents in large quantities, the resistance of these agricultural pests has been continuously enhanced, so that it is difficult to control their damage. It is estimated that the global annual losses caused by pesticides are as high as 100 billion U.S. dollars. With the development of scientific concepts, the scientific community and the public have new thinking on pest control. [0003] The purpose of pest...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P7/04A01G13/00
CPCA01G13/00A01N43/90
Inventor 邵雪花叶文才匡石滋王英赖多杨伟群赵天艺肖维强
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com