Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte

A pluripotent stem cell and induced differentiation technology, applied in the field of neural crest lineage pericytes and their induced differentiation, can solve the problems of different origins of pericyte development, uncontrolled differentiation paths, strong cell heterogeneity, etc., and achieve strong maintenance of blood vessels. Stabilizing capacity, enhancing cytological or therapeutic function, addressing source-limited effects
CN108949688AActive Publication Date: 2018-12-07SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2018-12-07

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Abstract

The invention discloses a neural crest-lineage pericyte derived from multi-potential stem cells and an induced differentiation method of the neural crest-lineage pericyte. The induced differentiationmethod comprises the following steps: dissociating the multi-potential stem cells to obtain single-cell suspension, inoculating the single-cell suspension into a matrigel-coated pore plate, and changing the suspension through an NCN2 culture medium each day; carrying out culturing for a certain period of time, carrying out digestion, and collecting cells; carrying out adherent culture on the collected cells, changing culture liquid into pericyte induced culture liquid, carrying out subculture, and detecting the cell phenotype of the pericyte. According to the induced differentiation method, the disadvantages of limited source of human cerebral vessel pericytes, and the heterogeneity and linkage heterogeniety of the pericytes derived from multi-potential stem cells obtained in other non-finite induction ways are solved, and the obtained neural crest-lineage pericyte has very strong blood vessel stabilizing capacity and contractility; and by virtue of a standardized induced differentiation procedure, cell populations obtained in different batches have good coherence.
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Description

technical field

[0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a neural crest lineage pericyte derived from pluripotent stem cells and a method for inducing differentiation thereof. Background technique

[0002] Pericytes, also known as Rouget cells or vascular wall cells, cover the outside of microvascular endothelial cells and exist in capillaries, postcapillary venules and precapillary arterioles. In recent years, the function of pericytes has attracted more and more attention. It plays an important role in the morphogenesis and function of blood vessels, cardiovascular homeostasis, and diseases such as ischemic stroke and diabetic retinopathy. However, there is no clear definition of pericytes at present. As a compromise, it is usually judged by comprehensive location, morphology, and gene / protein expression patterns.

[0003] Pericytes are ubiquitous in blood vessels. About 80% of microvessels in the b...

Claims

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