Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte

A pluripotent stem cell and induced differentiation technology, applied in the field of neural crest lineage pericytes and their induced differentiation, can solve the problems of different origins of pericyte development, uncontrolled differentiation paths, strong cell heterogeneity, etc., and achieve strong maintenance of blood vessels. Stabilizing capacity, enhancing cytological or therapeutic function, addressing source-limited effects

Active Publication Date: 2018-12-07
SUN YAT SEN UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the pericytes obtained by the methods reported in these literatures or patents are either differentiated from mesoderm, or the differentiation path is not controlled, and only focus on solving the source of pericytes, and do not provide corresponding soluti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte
  • Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte
  • Neural crest-lineage pericyte derived from multi-potential stem cells and induced differentiation method of neural crest-lineage pericyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Induction and differentiation method of neural crest lineage pericytes from pluripotent stem cells

[0039] The method for inducing differentiation of neural crest lineage pericytes from pluripotent stem cells in this embodiment comprises the following steps:

[0040] 1. In vitro adherent culture of pluripotent stem cells to maintain their undifferentiated state;

[0041] Pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, can be conveniently obtained commercially or constructed in our laboratory. This example focuses on further cultivation and differentiation operations based on a stably established pluripotent stem cell line (purchased from Wicell research institute).

[0042] Pluripotent stem cells can be expanded on a large scale without feeder layers and remain in an undifferentiated state. The culture medium can be selected from the mTeSR series products of STEMCELL, E8, etc. If it is compatible with the above-men...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a neural crest-lineage pericyte derived from multi-potential stem cells and an induced differentiation method of the neural crest-lineage pericyte. The induced differentiationmethod comprises the following steps: dissociating the multi-potential stem cells to obtain single-cell suspension, inoculating the single-cell suspension into a matrigel-coated pore plate, and changing the suspension through an NCN2 culture medium each day; carrying out culturing for a certain period of time, carrying out digestion, and collecting cells; carrying out adherent culture on the collected cells, changing culture liquid into pericyte induced culture liquid, carrying out subculture, and detecting the cell phenotype of the pericyte. According to the induced differentiation method, the disadvantages of limited source of human cerebral vessel pericytes, and the heterogeneity and linkage heterogeniety of the pericytes derived from multi-potential stem cells obtained in other non-finite induction ways are solved, and the obtained neural crest-lineage pericyte has very strong blood vessel stabilizing capacity and contractility; and by virtue of a standardized induced differentiation procedure, cell populations obtained in different batches have good coherence.

Description

technical field [0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a neural crest lineage pericyte derived from pluripotent stem cells and a method for inducing differentiation thereof. Background technique [0002] Pericytes, also known as Rouget cells or vascular wall cells, cover the outside of microvascular endothelial cells and exist in capillaries, postcapillary venules and precapillary arterioles. In recent years, the function of pericytes has attracted more and more attention. It plays an important role in the morphogenesis and function of blood vessels, cardiovascular homeostasis, and diseases such as ischemic stroke and diabetic retinopathy. However, there is no clear definition of pericytes at present. As a compromise, it is usually judged by comprehensive location, morphology, and gene / protein expression patterns. [0003] Pericytes are ubiquitous in blood vessels. About 80% of microvessels in the b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/079
CPCC12N5/0618C12N2501/135C12N2501/115
Inventor 项鹏李伟强孙佳琦
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap