Micro-fluidic chip for cell culturing and simulating post-exercise pulse shearing force environment, and detection method

Abstract

The invention relates to a micro-fluidic chip for cell culturing and simulating a post-exercise pulse shearing force environment, and a detection method, and belongs to the technical field of cell biomechanical experiment devices. The micro-fluidic chip comprises three parts: a cell culturing system, a shearing force waveform generating system and a shearing force waveform detecting system. The cell culturing system is formed by a cell culturing chamber, cell suspension liquid inlet / outlet and a microchannel; the shearing force waveform generating system is formed by a cell culturing chamber,cell culturing liquid inlet / outlet, an elastic chamber, a resistance channel and a microchannel; through adjusting a flow input waveform of the cell culturing liquid inlet and the size of a resistancechannel after changing, resting and loading of a post-exercise shearing force waveform are realized in the cell culturing chamber; the shearing force waveform detecting system is formed by pressure detecting microchannels on two sides of the cell culturing chamber, a liquid column change in the channel is detected through recording the pressure by a microscope, and the shearing force waveform inthe cell culturing chamber is further calculated through pressure values on two sides of the cell culturing chamber.

Description

technical field [0001] The invention belongs to the technical field of cell biomechanics experiment equipment, and is based on hemodynamic principles and microfluidic chip technology, and is used to study the influence of exercise-induced shear force signals on the morphology and function of vascular endothelial cells and its molecular biological mechanism The experimental device is specifically a microfluidic chip and a detection method for cell culture and simulated pulsating shear force environment after exercise. Background technique [0002] Vascular endothelial cells are located in the innermost layer of the blood vessel wall, the inner side is in direct contact with the flowing blood, and the outer side is adjacent to smooth muscle cells. Vascular endothelial cells are not only a selective physical barrier between blood and vascular tissue, they can also pass through receptors and receptors on the cell membrane surface, such as polysaccharide-protein complexes, tyrosi...

AI Technical Summary

Problems solved by technology

However, at present, the post-exercise shear stress waveform simulated by microfluidic chip technology is mainly steady flow shear stress, and a large number of in vitro experiments have confirmed that the biological responses of vascular endothelial cells to steady flow and pulsatile flow are significantly different.

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