Supercharge Your Innovation With Domain-Expert AI Agents!

Tetracycline sample pretreatment kit based on immunomagnetic beads

An immunomagnetic bead and tetracycline technology, which is applied in the field of food biological detection and analysis, can solve the problems of low separation efficiency, complicated purification and separation operations, etc., and achieves the effects of improving accuracy and precision, reducing the interference of background substances, and being easy to operate.

Pending Publication Date: 2018-12-11
沭阳康源泰博生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to make up for the deficiencies in the prior art, and to provide a tetracycline sample pretreatment kit for immunomagnetic beads, which is used for the enrichment and purification of tetracyclines in samples, so as to solve the purification and separation of tetracycline samples Technical problems such as complicated operation and low separation efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tetracycline sample pretreatment kit based on immunomagnetic beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of anti-tetracycline antibody immunomagnetic beads:

[0033] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the beads, repeat three times at 3 minutes intervals, remove the activation buffer, add 200μL each of the activator EDC and NHS, mix, and incubate at 37°C for 2 hours To activate magnetic beads;

[0034] 2. Wash the magnetic beads three times with coupling buffer at 3 minutes intervals, add tetracycline antibody, mix well, incubate at 37°C for 3 hours to prepare tetracycline antibody immunomagnetic beads;

[0035] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes. Incubate with blocking solution at 37°C for 2 hours, and store in storage buffer at 4°C until use;

[0036] 2. Application of anti-tetracycline immunomagnetic beads to the enrichment of tetracyclines in animal tissue samples:

[0037] 1. Mash the animal tissue sample with a meat masher, or chop it with a knife (pulp)...

Embodiment 2

[0042] 1. Preparation of anti-tetracycline antibody immunomagnetic beads:

[0043] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the beads, repeat three times at 5 minutes intervals each time, remove the activation buffer and add 180μL each of the activators EDC and NHS, mix well, and incubate at 37°C for 2 hours To activate magnetic beads;

[0044] 2. Wash the magnetic beads three times with coupling buffer at 3 minutes intervals, add tetracycline antibody, mix well, incubate at 37°C for 3 hours to prepare tetracycline antibody immunomagnetic beads;

[0045] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes. Incubate with blocking solution at 37°C for 2 hours, and store in storage buffer at 4°C until use;

[0046] 2. Application of anti-tetracycline immunomagnetic beads to enrichment of tetracycline in feed samples:

[0047] 1. Weigh 2.0 g of crushed feed sample, put it into a 5 mL centrifuge tube, add...

Embodiment 3

[0050] 1. Preparation of anti-tetracycline antibody immunomagnetic beads:

[0051] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the beads, repeat three times with an interval of 4 minutes each. After removing the activation buffer, add 250μL each of the activator EDC and NHS, mix well, and incubate at 37°C for 4 hours. To activate magnetic beads;

[0052] 2. Wash the magnetic beads three times with coupling buffer at 4 minutes intervals, add tetracycline antibody, mix, and incubate at 37°C for 4 hours to prepare tetracycline antibody immunomagnetic beads;

[0053] 3. Wash the immunomagnetic beads with washing buffer three times repeatedly with an interval of 4 minutes. Incubate with blocking solution at 37°C for 4 hours, and store in storage buffer at 4°C until use;

[0054] 2. Application of anti-tetracycline immunomagnetic beads to enrichment of tetracyclines in samples:

[0055] 1. Mash the animal tissue sample with a meat masher, or chop it wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides preparation and application of a tetracycline sample pretreatment kit based on immunomagnetic beads. The kit mainly consists of several parts including a sample cabin, a reagentcabin, a cleaning cabin, an elution cabin and the like. The operation method has the advantages that the efficiency and the speed are high; the operation is simple; large-scale instruments and specially trained professional operation personnel are not needed; the sample does not need special pretreatment, and can be widely applied to tetracycline enrichment and separation in products of animal tissues, water products, meat products and the like; for tetracycline samples with a value lower than the detection limit, through the enriching effects of immunomagnetic beads and the sufficient diffusion of the immunomagnetic beads in the liquid, the combination surface area is expanded; the reaction is thorough, so that the detection limit is changed; the detection sensitivity is improved; the inspection missing phenomenon is avoided.

Description

Technical field [0001] The invention belongs to the field of food biological detection and analysis, and specifically relates to a tetracycline sample pretreatment kit based on immunomagnetic beads. Background technique [0002] Tetracyclines are named after the similar molecular structure of the hydrogenated tetraphenyl ring. They are broad-spectrum antibiotics, and their use accounts for 57% of the total antibiotic use in my country. Because of its broad-spectrum and high-efficiency sterilization effect and low price, it can be used to prevent and treat fish diseases in the process of aquaculture. It can be used in the dairy industry to increase milk production in dairy cows. It can also prevent and treat livestock diseases. It can also be used as a feed additive to promote the growth of poultry and livestock, so it has a wide range of applications. However, excessive use of tetracycline antibiotics will inevitably leave mothers, metabolites and other related antibiotics in th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326G01N33/54346
Inventor 张波张学记郗日沫王鹏刘向阳雷达
Owner 沭阳康源泰博生物科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com