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Bioengineering bacteria for reducing divalent mercury ions and its preparation method and use

A technology of bioengineering and divalent mercury, which is applied in the field of water pollution control to achieve the effect of good selectivity, high sensitivity and low cost

Active Publication Date: 2022-01-28
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the bacteria used for biosorption will lose the ability to further reduce mercury pollution when the adsorption capacity reaches saturation.

Method used

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  • Bioengineering bacteria for reducing divalent mercury ions and its preparation method and use
  • Bioengineering bacteria for reducing divalent mercury ions and its preparation method and use
  • Bioengineering bacteria for reducing divalent mercury ions and its preparation method and use

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Embodiment Construction

[0041] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments, so that those skilled in the art can implement it with reference to the description.

[0042] The present invention provides a bioengineered bacterium for reducing divalent mercury ions, the bioengineered bacterium contains an expression vector, and the expression vector is merR op-ompA-merA-pSB1A2, which includes a recombinant gene merR op-ompA-merA and pSB1A2 plasmid backbone. The pSB1A2 plasmid backbone is an existing material that can be prepared by the prior art. In the present invention, it is a gift from the research group of Professor Zhao Jin of Nanjing University, which is used to construct a recombinant plasmid, that is, an expression vector. The plasmid map of the pSB1A2 plasmid backbone is as follows: figure 1 As shown, the rfp in the pSB1A2 map has been removed by the subsequent digestion reaction, which has no effect on the system c...

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Abstract

The invention discloses a bioengineering bacterium for reducing divalent mercury ions. The bioengineering bacterium contains an expression vector, the expression vector is merR op-ompA-merA-pSB1A2, which includes the recombinant gene merR op-ompA-merA and the pSB1A2 plasmid backbone. The invention also discloses a preparation method and application of a bioengineering bacterium for reducing divalent mercury ions. The bioengineering bacteria for reducing divalent mercury ions provided by the invention can be used to control Hg(II) pollution in water environment. The bioengineering bacteria are put into the water environment polluted by Hg(II), and the Hg(II) is reduced to Hg(0), so as to realize the treatment of the water environment. The bioengineering bacterium of the invention is used for treating Hg(II) pollution in the water environment, and has the advantages of ecological environment protection, good selectivity, high sensitivity, low cost and reusability.

Description

technical field [0001] The invention relates to the technical field of water pollution control, in particular to a bioengineered bacterium for reducing divalent mercury ions and a preparation method and application thereof. Background technique [0002] With the widespread use of mercury, a part of mercury enters the environment with the discharge of waste gas, waste water and waste residue, causing serious pollution to human production and living and the ecosystem, and even endangering human life and health. [0003] The biological toxicity of mercury is mainly caused by inorganic mercury and organic mercury compounds. The harm of organic mercury compounds to the human body is much greater than that of inorganic mercury, but the inorganic mercury ions in the current water pollution system are the main form of mercury pollution, and can be converted into organic mercury under the action of microorganisms. Therefore, it is particularly important to realize the treatment of i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/70C12N15/66C02F3/34C12R1/19C02F101/20
CPCC12N9/0091C12N15/52C12N15/66C12N15/70C02F3/34C02F2101/20
Inventor 王丹陈阳清韦娜金楠皓郑亚楠庞婷刘婷梁乐桂
Owner GUANGXI TEACHERS EDUCATION UNIV