A method for extracting hydroxytyrosol from olive pomace
A technology of olive fruit and hydroxytyrosol, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of complex components and low purity of hydroxytyrosol
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Embodiment 1
[0059] Precision weighing 7 servings of 1 g of olive slag, 25 ml of 0.3 mol / L of sodium hydroxide solution, respectively. The mixture was placed in a 80 ° C water bath to shock the water bath, and the hydrolysis time was 15 min, 30 min, 45 min, 60 min, 75 min, 90min, and obtained hydrolyzed reaction liquid. The hydrolyzing reaction liquid is centrifuged by centrifuge, and the solid component is removed to obtain a hydrolyzate. The hydrolyzate was added to 3 times volume of ethyl acetate and was shaken for 10 min. The supernatant was taken, and the lower layer was added to the same proportion to the extraction of ethyl acetate again, and the two extracts were prepared to obtain a hydroxybylol crude extract. The combined solution was evaporated to dryness, dried to give crude hydroxybylol.
[0060] After the obtained crude hydroxyhydroylene is incubated with methanol, a high purity hydroxy-yolflow extract can be obtained by preparing a high-pressure proximity to prepare. The escap...
Embodiment 2
[0070] Precision weighing 5 parts: 1 g of olive slag, 25 ml of 0.3 mol / L of sodium hydroxide solution, respectively. The mixture was placed in 50 ° C, 60 ° C, 70 ° C, 80 ° C, 90 ° C, and a water bath hydrolyzed for 30 min to obtain a hydrolyzed reaction liquid. The hydrolyzing reaction liquid is centrifuged by centrifuge, and the solid component is removed to obtain a hydrolyzate. The hydrolyzate was added to a salvation of acetate and was allowed to extract 10 min. The supernatant was taken, and the lower layer was added to the same proportion to the extraction of ethyl acetate, and the two extracts were prepared to obtain a hydroxybylol solution. The combined solution was evaporated to dryness, dried to give crude hydroxybylol.
[0071] The above-mentioned hydroxy-based alcohol was detected in accordance with the high-performance liquid chromatography as described in Example 1. Figure 4 Indicated.
[0072] Depend on Figure 4 It can be seen that the concentration of hydroxybyl ...
Embodiment 3
[0074] Precision weighing 8 parts by weight of 1 g of olive slag, 15 ml, 20 ml, 25 mL, 30 mL, 35 mL, and 40 ml of sodium hydroxide solution, respectively. The mixture was placed in a 80 ° C water bath to oscillate water moisture for 30 min to give a hydrolyzed reaction liquid. The hydrolyzing reaction liquid is centrifuged by centrifuge, and the solid component is removed to obtain a hydrolyzate. The hydrolyzate was added to a salvation of acetate and was allowed to extract 10 min. The supernatant was taken, and the lower layer was added to the same proportion to the extraction of ethyl acetate, and the two extracts were prepared to obtain a hydroxybylol solution. The combined solution was evaporated to dryness, dried to give crude hydroxybylol.
[0075] The above-mentioned hydroxy-based alcohol was detected in accordance with the ultra-efficient liquid chromatography described in Example 1. Figure 5 Indicated.
[0076] Depend on Figure 5 It can be seen that the increase in materi...
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