Application of ferulic acid or derivative thereof for preventing and treating plant bacteriosis or mycosis

A technology of plant bacteria and ferulic acid, applied in the field of pesticides, can solve the problems of large chemical pesticide pollution, easy resistance of pathogenic bacteria, poor environmental compatibility, etc., and achieve the effects of small environmental pollution, good market application prospects, and remarkable effects

Inactive Publication Date: 2018-12-21
BEIJING KINGBO BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Plant bacterial and fungal diseases are often controlled by chemical pesticides, but these chemical pesticides have problems such as large pollution, poor compatibility with the e

Method used

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  • Application of ferulic acid or derivative thereof for preventing and treating plant bacteriosis or mycosis
  • Application of ferulic acid or derivative thereof for preventing and treating plant bacteriosis or mycosis
  • Application of ferulic acid or derivative thereof for preventing and treating plant bacteriosis or mycosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Ferulic Acid Determination of Cucumber Bacterial Keratosis

[0033] 1. Bacterial species: Pseudomonas syringae pv. lachrymans, a pathogenic variety of Pseudomonas syringae pv. lachrymans.

[0034] 2. Concentration setting: Under sterile conditions, dilute ferulic acid with NB medium to form a series of culture solutions containing ferulic acid at 200, 100, 50, 25, 12.5, 6.25 and 3.125ug / mL. The blank control treated with medicine, each treatment was repeated 4 times.

[0035] 3. Test method: The turbidity method of the inhibition of bacterial growth test is used for determination. Dilute the bacteria of Xanthomonas japonica growing on the slant of NA medium to 1×10 with sterilized water. 7 1 spore / mL concentration of the suspension, inoculate 100 uL of bacterial solution into each treatment medium, and place it under the condition of 28-30° C. for shaking culture (120 r / min).

[0036] 4. Investigation and calculation: Measure the turbidity of each treatment...

Embodiment 2

[0040] Embodiment 2 ferulic acid is to capsicum phytophthora indoor determination

[0041] Preparation of test materials: Inoculate Phytophthora capsici Leonian on potato dextrose agar medium (PDA), and place it in a constant temperature incubator (25±1°C) for cultivation. Erlenmeyer flasks, glass rods, pipettes, culture dishes, punches, inoculators, etc. are sterilized and ready for use.

[0042] Drug configuration and treatment: Dissolve the original drug of ferulic acid with a small amount of acetone, and then dilute it with 0.1% Tween 80 aqueous solution to form 5 series of concentration gradients (respectively 4, 2, 1, 0.5, 0.25mg / L). Under aseptic operation conditions, according to the test treatment, quantitatively add the pre-thawed sterilized medium into the sterile Erlenmeyer flask, sequentially draw the liquid medicine from low concentration to high concentration, add it to the above-mentioned Erlenmeyer flask respectively, and shake well . Then pour the same amo...

Embodiment 3

[0048] Example 3 Derivatives of Ferulic Acid To Indoor Measurement of Phytophthora capsici

[0049] Preparation of test materials: Inoculate Phytophthora capsici Leonian on potato dextrose agar medium (PDA), and place it in a constant temperature incubator (25±1°C) for cultivation. Erlenmeyer flasks, glass rods, pipettes, culture dishes, punches, inoculators, etc. are sterilized and ready for use.

[0050] Drug preparation and processing: Dissolve ferulic acid derivatives with a small amount of acetone (the derivative numbers in Table 3 correspond to the ferulic acid derivative numbers in Table 1), and then use 0.1% Tween 80 aqueous solution to configure each The concentration of the derivative is 500ug / mL. Under aseptic conditions, according to the test treatment, quantitatively add the pre-thawed sterilized medium into the sterile Erlenmeyer flask, and add each derivative liquid into the above-mentioned Erlenmeyer flask respectively. Shake well. Then pour the same amount i...

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Abstract

The invention provides an application of ferulic acid or a derivative thereof for preventing and treating plant bacteriosis or mycosis. The ferulic acid or the derivative thereof is a compound with astructure as shown in a structure general formula (I). The formula is as shown in the description. It is discovered that the ferulic acid or the derivative thereof has activity of efficient plant fungi and bacteria resistance, is capable of effectively preventing and treating multiple types of crop bacteriosis and mycosis, has the remarkable effect of preventing and treating cucumber bacterial angular leaf spot, phytoph-thora capsici leonian, cucumber downy mildew and sclerotinia rot of colza especially, and has good market application prospect.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to the application of ferulic acid or its derivatives in the prevention and treatment of plant bacterial diseases or fungal diseases. Background technique [0002] Ferulic acid (FA), the chemical name is 4-hydroxy-3-methoxyphenylacrylic acid) is a ubiquitous phenolic acid, which cross-links with polysaccharides and lignin in plant cell walls to form a part of the cell wall. It is one of the active ingredients of traditional Chinese medicines such as Ferulicum, Angelica, Chuanxiong, Cimicifuga, etc. It has been widely used in medicine, health care products, cosmetic raw materials and food additives because of its strong antioxidant activity and antiseptic effect. In recent years, extensive and in-depth studies on its pharmacological effects have found that ferulic acid and its derivatives also have good antipyretic and analgesic properties, antithrombotic, blood lipid lowering, an...

Claims

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Application Information

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IPC IPC(8): C07C59/64C07C59/48C07C57/44C07D317/60C07D319/18C07C205/56C07C69/734C07C235/34C07H13/04C07D241/12C07C69/86C07C275/14C07D295/185C07D493/04A01N43/90A01N43/60A01N43/36A01N43/40A01N47/44A01N37/38A01N37/40A01N43/16A01N43/30A01N43/32A01P1/00A01P3/00
CPCA01N37/38A01N37/40A01N43/16A01N43/30A01N43/32A01N43/36A01N43/40A01N43/60A01N43/90A01N47/44C07C57/44C07C59/48C07C59/64C07C69/734C07C69/86C07C205/56C07C235/34C07C275/14C07D241/12C07D295/185C07D317/60C07D319/18C07D493/04C07H13/04
Inventor 陈爱端徐向荣石伶俐魏凤彪
Owner BEIJING KINGBO BIOTECH
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