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Method for jointly extracting citrus seed oil and limonoids from citrus seeds

A limonoid, combined extraction technology, applied in the directions of steroids, organic chemistry, fat oil/fat production, etc., can solve the problems of difficulty in reflecting commercial value, loss of collection and utilization value of citrus seed oil, inedible, etc., To achieve the effect of improving the edible properties of commodities, reducing the content and reducing the procurement cost

Active Publication Date: 2020-08-25
ZHEJIANG CITRUS RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The citrus seed oil produced by physical pressing method has a strong bitter taste due to a certain amount of limonin compounds, so it cannot be eaten at all, and its commercial value is difficult to reflect
And the citrus seed oil residue containing a lot of limonin can only be discarded as garbage
[0007] 2. Before using the organic solvent method to extract limonin, the dried citrus seeds are usually crushed and then extracted and degreased with toxic organic solvents such as petroleum ether and acetone. The oil contained in citrus seeds almost loses the value of collection and utilization.

Method used

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  • Method for jointly extracting citrus seed oil and limonoids from citrus seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Separation by pressing: Weigh 1000 g of citrus seeds, put them into a screw oil press and press repeatedly for more than 2 times until no oil seeps out, and collect 279.05 g of crude citrus seed oil and 717.90 g of citrus seed residue.

[0036] (2) Extraction of low-bitter citrus seed oil: Add 2000ml of 30% ethanol solution to the crude citrus seed oil, stir quickly and heat to 80°C for 30min, then keep it in a 4°C freezer for stratification. The upper layer is about 310ml of crude oil extracted for the first time, and the lower layer is about 1890ml of alcoholic extract for the first time, and is set aside. Add 1,200ml of 70% ethanol solution to the crude oil extracted for the first time, stir rapidly and heat to 60°C for 30 minutes of extraction, then place it in a refrigerator at 4°C for static stratification. Collect about 301ml of the oil layer, which is the low-bitter citrus seed oil. At the same time, 1205 ml of the second ethanol extract was collected for s...

Embodiment 2

[0042] (1) Separation by pressing: Weigh 1000 g of citrus seeds, put them into a screw oil press and press repeatedly for more than 2 times until no oil seeps out, and collect 272.11 g of crude citrus seed oil and 719.07 g of citrus seed residue.

[0043](2) Extraction of low-bitter citrus seed oil: Add 2000ml of 20% ethanol solution to the crude citrus seed oil, stir rapidly and heat to 85°C for 30min, then keep it in a 6°C freezer for stratification. Collect the upper layer to be about 319ml of crude oil for the first time, and the lower layer is about 1900ml for the first alcoholic extraction, and set aside. Add 1200ml of 80% ethanol solution to the crude oil extracted for the first time, stir rapidly and heat to 70°C for 30min, keep it warm for extraction, then place it in a refrigerator at 6°C for static stratification. Collect about 298ml of the oil layer, which is the low-bitter citrus seed oil. At the same time, 1210 ml of the second ethanol extract was collected for ...

Embodiment 3

[0049] (1) Separation by pressing: Weigh 1000g of citrus seeds, put them into a screw oil press and press repeatedly for more than 2 times until no oil seeps out, and collect 250.50g of crude citrus seed oil and 739.00g of citrus seed residues.

[0050] (2) Extraction of low-bitter citrus seed oil: Add 2000ml of 10% ethanol solution to the crude citrus seed oil, stir quickly and heat to 90°C for 30min, keep it for extraction, then place it in a freezer at 8°C for stratification. The upper layer is about 275ml of crude oil extracted for the first time, and the lower layer is about 2000ml of alcoholic extract for the first time, and is set aside. Add 1,200ml of 90% ethanol solution to the crude oil extracted for the first time, stir rapidly and heat to 65°C for 30 minutes of extraction, then place it in a freezer at 8°C for static stratification. Collect about 245ml of the oil layer, which is the low-bitterness citrus seed oil. At the same time, 1250 ml of the second ethanol ex...

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Abstract

The invention belongs to the technical field of extraction of compounds from plants, and relates to a united extraction method of citrus seed oil and limonin compounds from citrus seeds. The united extraction method comprises the following steps of performing squeezing to separate citrus seed oil crude products from citrus seed residues; performing ethanol extraction on the citrus seed oil crude products twice with high-concentration ethanol and low-concentration ethanol so as to obtain low-bitterness citrus seed oil; performing ethanol extraction on the citrus seed residues with low-concentration ethanol, and then performing water extraction; combining ethanol extraction liquid of the low-concentration ethanol twice, performing decompression, and performing evaporation to dryness to obtain nomilin; and combining ethanol extraction liquid of the high-concentration ethanol with water extraction liquid, performing decompression, and performing evaporation to dryness so as to obtain limonin. According to the united extraction method of citrus seed oil and limonin compounds from citrus seeds provided by the invention, the content of the limonin compounds in the prepared citrus seed oilis low, and bitterness is inapparent; and besides, main limonin compounds-the limonin and the nomilin are subjected to preliminary separation, the oil yield of the citrus seed oil is not influenced,the mouth feel of oil products can also be improved, and sufficient utilization of resources is achieved.

Description

technical field [0001] The invention belongs to the technical field of extracting compounds from plants, and relates to a method for jointly extracting citrus seed oil and limonoid compounds from citrus seeds. Background technique [0002] Citrus seeds are the seeds of citrus plants in the family Rutaceae, generally accounting for 0.1% to 4.0% of the weight of citrus fruits. In my country's citrus processing industry, about 40,000 tons of citrus seed waste will be generated every year. But citrus seeds are not useless, it is not only rich in oil (38.86%-42.59%), protein (28.56%-36.20%), etc., but also contains limonoids, flavonoids and other bioactive components, especially limonin The anti-inflammatory, analgesic, regulating cholesterol levels in the body, preventing atherosclerosis and inhibiting tumor cells make citrus seeds have broad prospects in the application of functional foods and pharmaceutical raw materials. [0003] Citrus seed oil is mainly composed of fatty a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J73/00C11B1/06C11B3/00
CPCC07J73/008C11B1/06C11B3/006
Inventor 曹雪丹方修贵王天玉赵凯王鹏
Owner ZHEJIANG CITRUS RES INST