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A kind of bacterial agent and sensitive technology, applied in the field of pH-sensitive agricultural bacterial agent, can solve the problems of reducing fertilizer use efficiency, fertilizer loss, unfavorable crop growth, etc., to improve nutrient supply, inhibit activity, and improve soil salinity effect on the problem
Active Publication Date: 2018-12-21
长沙昱旻信息科技有限公司
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Problems solved by technology
Salinized soil is not conducive to the growth of crops, and a large number of hydrogen ions in acidic soil lead to the loss of some fertilizers
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Embodiment 1
[0045] Embodiment 1, the preparation of pH-sensitive agricultural bacterial agent
[0046] (1) Preparation of agricultural fungicide granules
[0047] Add Bacillus subtilis, yeast, lactic acid bacteria, starch, and water with a mass ratio of 2:2:2:2:1 to a high-speed stirring granulator to granulate, boil and dry at 30-37°C to a water content of 3-5%, and pass 20 Mesh sieve to obtain agricultural fungus granules.
[0048] (2) Dopamine is coated on the surface of agricultural fungus granules
[0049] Weigh 1.5g Tris and place it in a beaker, add about 900mL of deionized water, adjust the pH of the Tris-HCl solution to 8-8.5 with 6M HCl; add 50g of the above-prepared agricultural fungus granules to the above-mentioned Tris-HCl solution, and dilute the solution to 1 L; add 5 g of dopamine hydrochloride, stir at room temperature for more than 2 hours, and suction filter to obtain agricultural fungicide particles coated with dopamine on the surface.
[0050] (3) pH-sensitive pol...
Embodiment 2
[0053] Embodiment 2, the living bacteria release experiment of pH-sensitive agricultural bacterial agent
[0054] Get 6g of pH-sensitive agricultural fungal agent granules prepared according to the method described in Example 1, divide them into 3 parts on average, place them in sterile water with a pH value of 5, 7, and 9 for 1 day, and then detect different pH values. In some cases, the release amount of live bacteria of the pH-sensitive bacterial agent.
[0055] Plate detection method to detect the release of viable bacteria detection method:
[0056] 1) Prepare a solution according to the mass volume ratio of the placed sample: sterile water at a ratio of 1:10, and filter the solution through a 3-micron filter membrane to filter out the dopamine attached to the surface of the unreleased live bacteria Bacteria particles and bacteria particles coated with polymer molecules;
[0057] 2) Collect the filtrate, add glass beads, let it stand for 20 minutes, and shake it on a ro...
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Abstract
The invention provides a pH-sensitive agricultural microbial agent. The microbial agent is prepared from a microorganism, a binder, a dopamine or a salt thereof, and a pH-sensitive high polymer molecule. The pH-sensitive high polymer molecule is a high polymer molecule polymerized by an acrylic acid by using graphene oxide as a crosslinking agent. According to the invention, the dopamine is attached to a surface of the living granulating agent, then the pH-sensitive high polymer molecule are covered on a surface of the dopamine, so as to prepare the pH-sensitive agricultural microbial agent that a release of living bacteria of the agent can be adjusted according to the pH of the environment. In the saline-alkaline soil, by applying the pH-sensitive agricultural microbial agent provided bythe invention, the living bacteria can be quickly released, so as to accelerate the improvement of soil salinization problem; while applying the pH-sensitive agricultural microbial agent provided by the invention in the acidic soil, the release rate of the live bacteria can be effectively slowed down, so as to prevent the loss of the organic fertilizer produced by the decomposition of the living bacteria in the acidic soil, and to improve the utilization efficiency of the fertilizer.
Description
technical field [0001] The invention belongs to the technical field of fertilizer development, and in particular relates to a pH-sensitive agricultural bacterial agent. Background technique [0002] Generally, the climate varies greatly across my country. With the large-scale development of sustainable modern agriculture, the excessive use of fertilizers and human activities have caused more and more serious problems in terms of soil acidity and alkalinity. The physical and chemical properties of soil have a significant impact on the growth environment of plants and the utilization efficiency of fertilizer itself. Salinized soil is not conducive to the growth of crops, and a large number of hydrogen ions in acidic soil lead to the loss of some fertilizers, which greatly reduces the utilization efficiency of fertilizers. For the solution of above-mentioned problem, also do not have an effective means to propose at present. Contents of the invention [0003] The invention ...
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