Method for screening acidophilic heavy metal-resistant nitrogen-fixing bacteria from soil
A technology of heavy metals and nitrogen-fixing bacteria, which is applied in the field of environmental microorganisms, can solve the problems of difficulty in meeting the needs of acidic soil treatment, low screening efficiency, poor ability of acidophilus and heavy metal resistance, and achieve good biological strain resources, improved screening efficiency, and acidophilic tolerance. The effect of heavy metal capacity enhancement
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[0045] Example 1
[0046] A method for screening acidophilic and heavy metal-resistant nitrogen-fixing bacteria from soil, which can include the following steps:
[0047] Step A1, take 10g of fresh acidic soil into a 250m conical flask, add 100mL of sterile water, and shake at 150rpm and 30°C constant temperature for 24h, then centrifuge at 4000rpm and discard by decantation The supernatant was obtained to obtain a precipitate containing mixed strains.
[0048] Step B1: Add 100 mL of sterile acidic culture solution to the conical flask containing the sediment of mixed strains, and shake and culture at 150 rpm and 30°C for 2 hours to obtain a bacterial strain mixture. Pipette 10mL of this strain mixture as the inoculum, and perform step C1.
[0049] Step C1, adding the inoculated bacteria into 100 mL of sterile acidic culture solution, and shaking at 150 rpm and a constant temperature of 30 °C for 2 hours to obtain a bacterial culture solution; draw 10 mL of the bacterial cul...
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