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Method for screening acidophilic heavy metal-resistant nitrogen-fixing bacteria from soil

A technology of heavy metals and nitrogen-fixing bacteria, which is applied in the field of environmental microorganisms, can solve the problems of difficulty in meeting the needs of acidic soil treatment, low screening efficiency, poor ability of acidophilus and heavy metal resistance, and achieve good biological strain resources, improved screening efficiency, and acidophilic tolerance. The effect of heavy metal capacity enhancement

Active Publication Date: 2018-12-21
安徽省交通航务工程有限公司 +2
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Problems solved by technology

However, in the prior art, the screening method for acidophilic heavy metal-resistant bacterial strains has low screening efficiency, poor acidophilic heavy metal resistance, and is difficult to meet the needs of acidic soil management.

Method used

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Embodiment 1

[0046] A method for screening acidophilic and heavy metal-resistant nitrogen-fixing bacteria from soil may comprise the following steps:

[0047] Step A1: Take 10g of fresh acidic soil in a 250m Erlenmeyer flask, add 100mL of sterile water, shake and cultivate at 150rpm at a constant temperature of 30°C for 24h, then centrifuge at 4000rpm, discard by decantation supernatant to obtain a precipitate containing mixed strains.

[0048] Step B1. Add 100 mL of sterile acidic culture solution to the Erlenmeyer flask containing the precipitate containing the mixed strains, and vibrate at 150 rpm at a constant temperature of 30°C for 2 hours to obtain a mixed solution of strains. Take 10mL of the strain mixture as the inoculum, and perform step C1.

[0049] Step C1, adding the inoculum to 100mL sterile acidic culture solution, and vibrating at 150rpm at a constant temperature of 30°C for 2h to obtain the culture solution of the strain; draw 10mL of the culture solution of the strain a...

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Abstract

The invention discloses a method for screening acidophilic heavy metal-resistant nitrogen-fixing bacteria from soil. Acid soil is mixed with water for shaking culture and centrifugal separation, supernatant is removed, and then a sterile acidic cultural solution is added for shaking culture, strain mixed liquor is obtained; the strain mixed liquor is taken as an inoculant. The sterile acidic cultural solution is added to the inoculant for shaking culture to obtain a strain culture solution; the strain culture solution is taken as an inoculant, and the inoculant is used for performing the shaking culture again until the shaking culture is performed for 6 times; the strain culture solution obtained from the fourth, fifth and sixth shaking culture is sucked, and added to a nitrogen-fixing medium for plate spreading culture and slant culture bacteria, and then the strain is separated and purified to obtain purified acidophilic heavy metal-resistant nitrogen-fixing bacteria. The method forscreening the acidophilic heavy metal-resistant nitrogen-fixing bacteria from soil can quickly and efficiently screen the acidophilic heavy metal-resistant nitrogen-fixing bacteria suitable for acid soil, and can greatly improve strain culture harvest rate and acidophilic heavy metal-resistant ability of the strain.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a method for screening acidophilic and heavy metal-resistant nitrogen-fixing bacteria from soil. Background technique [0002] Long-term large-scale application of chemical fertilizers will cause soil acidification, and soil acidification will promote the dissolution and release of harmful heavy metals in the soil, which will promote the absorption and accumulation of heavy metals by crops and aggravate the harm of heavy metals to the human body. [0003] At present, the use of biological techniques to improve the quality of acidified soil (for example: the use of acidophilic heavy metal-resistant biological strains to improve the quality of acidified soil) is a hot research direction. Soil microorganisms have a strong recovery ability and can be used to restore the diversity of microorganisms in acidified soils, improve the structure of soil microbial commun...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/065
CPCC12N1/02C12N1/20
Inventor 冯银炉孙庆业邵佳崔林钊
Owner 安徽省交通航务工程有限公司
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