Three-dimensional imaging device based on K space transformation and imaging method thereof
A 3D imaging and K-space technology, applied in the field of 3D imaging, can solve problems such as difficulty in implementation, and achieve the effects of eliminating mutual crosstalk, high device stability, and simple optical path
Active Publication Date: 2018-12-25
SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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Problems solved by technology
Since the lighting system and the acquisition system are not coaxial systems, this technology is difficult to implement
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[0037] The present invention will be further described below in conjunction with the examples and drawings, but the examples should not limit the protection scope of the present invention.
[0038] please participate first figure 1 , figure 1 It is a schematic diagram of the three-dimensional imaging method based on K-space transformation. As shown in the figure, the laser light emitted by laser 1 with a wavelength of 632.8nm passes through lens 2 to become a beam of parallel light with a flat phase, and then passes through a beam-splitting prism with a beam-splitting ratio of 1:1. 3 Split the light beam to produce two beams of parallel light with equal intensity, which are respectively the probe beam and the reference beam. The sheet-shaped illumination light is irradiated on the sample holder 10 to be tested; the sample holder to be tested is controlled by a one-dimensional electric displacement stage 9, and the light of the reference optical path is split by the beam split...
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Abstract
The invention discloses a three-dimensional imaging method based on K space transformation. The method comprises: making an interference by using the same sheet shape light source with the light illumination by adopting a sheet shape light illumination sample along the optical axis direction,; recording an interferogram and obtaining the complex amplitude information (including amplitude and phase) of the illuminated sample on a focal plane; the spatial spectrum information of the sample can be obtained by discrete Fourier transform; then using a projection method to calculate the spatial spectrum information perpendicular to the plane of the sample; obtaining the intensity information perpendicular to the plane of the sample by using discrete inverse Fourier transform; and finally, obtaining a final sample three-dimensional structure information through scanning. The imaging method has higher acquisition rate and higher resolution; imaging can be achieved with only one single acquisition when acquiring axial information.
Description
technical field [0001] The invention relates to the field of three-dimensional imaging, in particular to a three-dimensional imaging device and an imaging method based on K-space transformation. Background technique [0002] 3D imaging technology has grown rapidly due to its widespread adoption. This technology plays a great role in the observation of biological samples and tumor diagnosis. There are many methods used in the field of 3D imaging, such as structured light illumination techniques, confocal scanning, coherence tomography, sheet illumination microscopy and coherence tomography. These techniques can reflect the internal structure of the sample, including the reflectivity of the sample, the concentration of luminescent materials and other information. Structured light 3D imaging technology uses a carrier frequency fringe illumination light to irradiate the object, records the deformed fringe, and then digitally demodulates and reconstructs the 3D image of the mea...
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IPC IPC(8): G01N21/39G01N21/47
CPCG01N21/39G01N21/4788G01N2021/479
Inventor 张雪丹刘诚朱健强
Owner SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI