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Method for distinguishing organic rice and non-organic rice by utilizing proteomics technology

A technology of organic rice and proteomics, applied in the field of grain identification, can solve the problems of inefficient detection of organic rice and non-organic rice, and achieve accurate and reliable detection results, convenient operation, and high convincing effect

Active Publication Date: 2018-12-28
HARBIN INST OF TECH
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Problems solved by technology

[0004] In order to solve the problem that existing chemical methods cannot quickly and efficiently detect organic rice and non-organic rice, the present invention provides a method for distinguishing organic rice and non-organic rice using proteomics technology. The technical scheme adopted is as follows:

Method used

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  • Method for distinguishing organic rice and non-organic rice by utilizing proteomics technology
  • Method for distinguishing organic rice and non-organic rice by utilizing proteomics technology
  • Method for distinguishing organic rice and non-organic rice by utilizing proteomics technology

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Experimental program
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Embodiment 1

[0049] 1. Experimental method (technical roadmap such as figure 1 shown)

[0050] 1. Sample

[0051] 26 rice samples were selected for the experiment, including 13 organic rice samples and 13 ordinary rice samples. According to the Kennard-Stone algorithm, each rice sample was randomly divided into a calibration set (10) and a validation set (3) in a ratio of 3:1.

[0052] 2. Protein extraction

[0053] The sample was ground into powder with liquid nitrogen and transferred to a 50ml centrifuge tube, and STD buffer (4% SDS, 1mM DTT, 150mM Tris-HCl, pH8.0) was added at a ratio of 10:1, mixed with Votex, and placed in a boiling water bath for 5 minutes. Sonicate (80w, 10s, 15s interval, 10 times in total) in a boiling water bath for 5min, centrifuge to take the supernatant, and obtain crude protein extract. Use the BCA method to detect the total protein content in the crude protein extract, take about 20 μg of SDS-PAGE electrophoresis for detection, and if the protein can be ...

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Abstract

The invention discloses a method for distinguishing organic rice and non-organic rice by utilizing a proteomics technology, and belongs to the technical field of grain identification. The method comprises the following steps of performing protein extraction on an organic rice sample, a non-organic rice sample and rice to be detected separately so as to obtain a crude protein extracting solution; carrying out enzymolysis on the crude protein extracting solution by utilizing trypsin to obtain enzymatic hydrolysate; carrying out separation and identification on the enzymatic hydrolysate by capillary liquid chromatography cascaded triple quadrupole mass spectrometry to obtain protein information; carrying out deletion screening on the protein information of the organic rice and the non-organicrice to obtain difference protein of the organic rice and the non-organic rice, and establishing a partial least squares-discriminant analysis model according to the content of the difference proteinof each organic rice sample and non-organic rice sample in the enzymatic hydrolysate; and substituting the to-be-detected rice data into the model for identification. According to the method, organicrice and non-organic rice can be effectively distinguished, and the result is visual, accurate and reliable.

Description

technical field [0001] The invention relates to a method for distinguishing organic rice and non-organic rice by using proteomics technology, and belongs to the technical field of grain identification. Background technique [0002] As one of the "omics" technologies (including genomics, proteomics, transcriptomics and metabolomics), proteomics is used to clarify life phenomena and study the laws of life activities from the overall expression level. Compared with static genomics research, proteomics is a dynamic cell dynamics research with time and space, so this new discipline is also regarded as a new "post-genome" research method for exploring Specific gene-protein interactions and modified protein-protein interactions. In recent years, proteomics has been widely used in the identification and tracking of plant response to environmental stress. [0003] Rice is an important cereal crop, especially in Asia. In recent years, with the gradual improvement of people's living...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 马莺肖然李琳何胜华王荣春
Owner HARBIN INST OF TECH
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