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Method for constructing microbial co-culture system to highly produce pleurotus tuber-regium

A technology of co-cultivation system and tiger milk mushroom cells is applied in the field of constructing a microbial co-cultivation system to produce high-yield tiger milk mushroom extracellular polysaccharide, which can solve the problems of undiscovered polysaccharide yield and the like, and achieve the effects of simple process, improved solubility and increased yield.

Active Publication Date: 2019-01-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no application examples have been found to improve the yield of polysaccharides by using the mixed fermentation of lactic acid bacteria and macrofungi

Method used

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  • Method for constructing microbial co-culture system to highly produce pleurotus tuber-regium
  • Method for constructing microbial co-culture system to highly produce pleurotus tuber-regium
  • Method for constructing microbial co-culture system to highly produce pleurotus tuber-regium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Cut the PDA solid medium covered with tiger milk mushroom mycelium into 1cm 2 Add the small pieces into the sterilized seed culture solution, cultivate at 180rpm, 30°C for 3 days, insert the sterilized liquid fermentation medium for liquid fermentation, the inoculum size is 5.0%, 180rpm, the temperature is 30°C, and the fermentation time is 7 days. Wherein the filling volume of seed liquid is 100mL / 250mL triangular flask, and the loading volume of fermentation broth is 190mL / 500mL.

[0024] Lactic acid bacteria seed solution was pre-cultured in MRS medium at 100 rpm at 37°C for 18 hours. The lactic acid bacteria seed solution with 5.0% of the total volume of the tiger milk mushroom fermentation liquid was inoculated into the tiger milk mushroom fermentation liquid, the inoculation time was the 6th day of the tiger milk mushroom liquid fermentation, the co-cultivation temperature was 30°C, and the co-cultivation speed was 180rpm. Fermentation was finished when the tota...

Embodiment 2

[0028] Cut the PDA solid medium covered with tiger milk mushroom mycelium into 1cm 2 Add the small pieces into the sterilized seed culture solution, cultivate at 180rpm, 30°C for 3 days, insert the sterilized liquid fermentation medium for liquid fermentation, the inoculum size is 10%, 160rpm, the temperature is 30°C, and the fermentation time is 7 days. Wherein the filling volume of seed liquid is 90mL / 250mL triangular flask, and the loading volume of fermentation broth is 180mL / 500mL.

[0029] The lactic acid bacteria seed liquid was pre-cultured in MRS medium for 24 hours, the culture temperature was 37°C, and the shaker speed was 100rpm. The lactic acid bacteria seed solution with 2.5% of the total volume of the tiger milk mushroom fermentation liquid was inoculated in the tiger milk mushroom fermentation liquid, the inoculation time was the 5th day of the tiger milk mushroom fermentation time, the co-cultivation temperature was 30°C, and the co-cultivation speed was 180r...

Embodiment 3

[0033] Cut the PDA solid medium covered with tiger milk mushroom mycelium into 1cm 2 Add the small pieces into the sterilized seed culture solution, cultivate at 180rpm, 30°C for 3 days, insert the sterilized liquid fermentation medium for liquid fermentation, the inoculum size is 5.0%, 180rpm, the temperature is 30°C, and the fermentation time is 7 days. Wherein the filling volume of seed liquid is 100mL / 250mL triangular flask, and the loading volume of fermentation broth is 190mL / 500mL.

[0034] Lactic acid bacteria seed solution was pre-cultured in MRS medium at 100 rpm at 37°C for 18 hours. The lactic acid bacteria seed liquid with 5.0% of the total volume of the tiger milk mushroom fermentation liquid was inoculated in the tiger milk mushroom fermentation liquid, the inoculation time was the 5th day of the tiger milk mushroom liquid fermentation, the co-cultivation temperature was 30°C, and the co-cultivation speed was 180rpm. End the fermentation when the total fermentat...

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Abstract

The invention discloses a method for constructing a microbial co-culture system to highly produce pleurotus tuber-regium, and belongs to the technical field of bio-engineering. Pleurotus tuber-regiummycelium and lactic acid bacteria are co-cultured, and the inoculum size and co-culture time of lactic acid bacteria during co-culture are optimized, so that the yield of polysaccharides is increased,and dissolving property of the polysaccharides is improved. Strains used by the method are edible strains, and are safe and reliable; the used culture medium raw materials are cheap and easily available, and are free of pollution, so that realization is easy. The method is simple and convenient in process, is high in operability, and can realize large-scale and industrialized production.

Description

technical field [0001] The invention relates to a method for constructing a microbial co-cultivation system to high-yield exopolysaccharide of tiger milk mushroom, belonging to the technical field of bioengineering. Background technique [0002] Tiger milk mushroom (Pleurotus tuber-regium), scientific name Pleurotus sclerotium, also known as tiger milk fungus, nuclear ear mushroom, Pleurotus tuckahoe, Nanyang tuckahoe, etc., belongs to Basidiomycotina, Phylomycetes, Agaricaceae, Pleurotaceae, Pleurotus The genus is a rare edible and medicinal fungus mainly distributed in tropical and subtropical regions. Tiger milk mushroom not only has extremely high nutritional value, but also is rich in various biologically active components. Polysaccharide, as the component with the highest content, has attracted much attention in recent years. Studies have shown that tiger milk mushroom polysaccharide can significantly improve the immune function of the body, and has good application p...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/645
CPCC12P19/04C12P39/00
Inventor 陈磊葛梦蝶林俊德张薄博
Owner JIANGNAN UNIV
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