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Aspergillus oryzae and application thereof

A technology of Aspergillus oryzae and strains, applied in water/sludge/sewage treatment, biochemical equipment and methods, microorganisms, etc., can solve problems such as metabolic process disorders, difficulties, and human health impacts, and achieve high application value and strong removal effect of ability

Active Publication Date: 2019-01-04
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that atrazine can cause damage to the metamorphosis and morphological development of frogs, and even in surface water where low concentrations of atrazine exist, it can also have harmful effects on the development of amphibians; It will cause disturbance of important metabolic processes in the body; at a certain dose, it may cause genetic damage to the germ cells of mice, and interfere with the normal production and maturation of sperm; atrazine will not only affect human health through the transmission of food chains, Make the liver, kidney, lung, heart and blood vessels of the human body show symptoms of poisoning, and may also cause problems with the human cardiovascular system and difficulties in regeneration and reproduction

Method used

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  • Aspergillus oryzae and application thereof
  • Aspergillus oryzae and application thereof
  • Aspergillus oryzae and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The medium (separation medium) used in the present invention:

[0043] Beef extract glucose medium: beef extract 5g, glucose 10g, sodium chloride 10g, atrazine 200mg, distilled water 1000mL, agar powder 20g.

[0044] The fermentation medium used in the present invention: 5g of beef extract, 10g of glucose, 10g of sodium chloride, 1000mL of distilled water, pH 7±0.2.

[0045] The original bacterial strain ECUST-TXZC2018 of the present invention is obtained by separating and screening from atrazine-contaminated soil; the method for isolating and cultivating the bacterial strain comprises the following steps:

[0046] Isolation of strain Aspergillus oryzae: 10g of soil from atrazine-contaminated area was put into 90mL liquid beef extract glucose medium containing 200mg / L atrazine and 500mg / L atrazine for enrichment culture , after 48h, the enriched culture solution was taken for 10 -4 、10 -5 、10 -6 For gradient dilution, 0.1 mL each was spread on the atrazine-containin...

Embodiment 2

[0056] The cultivation method of Aspergillus oryzae: the strain Aspergillus oryzae is inoculated into the fermentation medium, and cultured with shaking at 30±2°C and 220 rpm for 4-5 days. The composition of the fermentation medium is 5g of beef extract, 10g of glucose, 10g of NaCl and 1000mL of distilled water, pH 7±0.2.

Embodiment 3

[0058] Research on the characteristics of strains adsorbing atrazine: the experiment of the influence of pH, adsorption time and temperature on the adsorption effect.

[0059] The strain was expanded and cultivated in beef extract glucose medium at 28°C and 220rpm for 6h, the obtained activated strain was centrifuged at 8000rpm for 10min, the obtained pure strain was washed 2-3 times with sterile water / dilute nitric acid, and prepared as Concentrated bacteria solution for use. Using 1mol / L NaOH and HCl to adjust the pH of the solution containing 200mg / L atrazine to be 1, 3, 5, 7, 9 respectively; the temperature is 15, 20, 25, 30, 35°C; v / v) The prepared concentrated bacterial liquid is placed in the atrazine-containing bacterial liquid prepared above, and the supernatant is taken regularly to measure the atrazine content.

[0060] (1) Adsorption time: the result is as follows Figure 4 as shown, Figure 4 is the schematic diagram of the effect of time on the adsorption rate...

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Abstract

The invention discloses Aspergillus oryzae. The fungus strain Aspergillus oryzae is prepared in China General Microbiological Culture Collection Center, and the preservation number of the Aspergillusoryzae is CGMCC No. 15680. A culture method of the Aspergillus oryzae includes: inoculating the strain to a fermentation culture medium, and performing shake culture under the temperature of 30+ / -2 DEG C and the rotation speed of 220rpm for 4-5 days. The screened Aspergillus oryzae is applied to the treatment of soil and water polluted by atrazine. During the treatment of the water or soil polluted by low-concentration atrazine, a method using the Aspergillus oryzae is capable of effectively and fast removing the atrazine pollution, green, environmentally friendly and economical as compared aphysical and chemical remediation method. The Aspergillus oryzae has a quite high application value in the treatment of the water and soil polluted by atrazine and the comprehensive treatment of industrial site pesticide pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an aspergillus oryzae which absorbs and tolerates atrazine. Background technique [0002] Today, as the world's population continues to grow, people are increasingly dependent on synthetic chemical pesticides. A large amount of chemical pesticides are required to be used in agricultural production every year. Some of these chemicals have an effect, while a large part remains in the environment, resulting in the deterioration of the quality of soil and agricultural products, and at the same time threatening human health. For pesticide pollution, the traditional treatment methods mainly focus on chemical remediation, physical-chemical remediation, and improved agronomic measures. These methods can solve the problem of soil pollution to a certain extent, but they also have high costs and may cause secondary pollution, etc. question. In recent years, the use of biodegradati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34B09C1/10C12R1/69C02F101/38
CPCB09C1/105C02F3/347C02F2101/38C12N1/14C12N1/145C12R2001/69
Inventor 徐文平常远森陶黎明张阳郝有武杨韵张楠李延娣
Owner EAST CHINA UNIV OF SCI & TECH
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