Optical nanostructure probe and single cell colloid osmotic pressure detection platform and manufacturing method of optical nanostructure probe

Abstract

The invention discloses an optical nanostructure probe, a single cell colloid osmotic pressure detection platform using the probe and a manufacturing method of the optical nanostructure probe, whereinthe detection platform comprises a sample cell, a microscopic device, an optical nanostructure probe, the optical nanostructure probe is fixed on a micro-operation device, the micro-operation deviceis connected with a control device, a light source device is connected with the control device, an output end for inputting a nano optical fiber and light inlet end for outputting the nano optical fiber of the optical nanostructure probe puncture into a sample cell in a micro pit, a light outlet end for outputting the nano optical fiber is connected with a photon detection device, and a data acquisition and analysis device converts the colloid osmotic pressure of the sample cell according to the photon intensity information at the light outlet end of the nanometer optical fiber. According to the invention, the detection of the living cells can be achieved, the influence of cell death and treatment on the real detection of the colloid osmotic pressure in the cell is avoided, and the reliability of measurement data is higher.

Description

technical field [0001] The invention relates to the field of optical detection, in particular to an optical nanostructure probe, a single-cell colloid osmotic pressure detection platform using the probe, and a manufacturing method of the optical nanostructure probe. Background technique [0002] At present, the instruments used to measure the osmotic pressure of solutions are mainly divided into four types: freezing point osmometers, dew point osmometers, membrane osmometers and colloid osmometers. Wherein, both the freezing point osmometer and the dew point osmometer measure the overall osmotic pressure of the solution. When using a freezing point osmometer or dew point osmotic pressure to measure the total osmotic pressure, it is necessary to undergo specific operations (such as protein denaturation, etc.) before calculating the crystal osmotic pressure and colloid osmotic pressure in the total osmotic pressure. Membrane osmometers measure the osmotic pressure of macromol...

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Problems solved by technology

[0006] The object of the present invention is to provide a single-cell colloid osmotic pressure detection platform based on optical nanostructure probes and a method for making the probes, so as to solve the problem of breaking cells and extracting cytoplasm to measure intracellular osmotic pressure in the prior art. Unable to measure colloid osmotic pressure in living single cells

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