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Determining method for mutual effect of metal ions and ligands

A metal ion and determination method technology, applied in the field of analysis and testing, can solve problems such as inability to perform effective screening and ligand screening interference, and achieve the effects of simple operation, fast and accurate screening, and accurate judgment

Inactive Publication Date: 2019-01-04
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, effective screening cannot be performed for ligands that do not contain UV-absorbing groups
In addition, the solvent environment during ligand screening often has characteristic absorption in the ultraviolet spectral region, which will interfere with ligand screening, so it is necessary to design an assay method that can solve the above problems

Method used

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  • Determining method for mutual effect of metal ions and ligands
  • Determining method for mutual effect of metal ions and ligands
  • Determining method for mutual effect of metal ions and ligands

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1, configure 25mmol / L of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 7.24, weigh 0.1299g of sodium arsenite, and carry out in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution After configuration, a 10 mmol / L sodium arsenite solution was obtained, and then diluted to obtain a 1 mmol / L sodium arsenite solution.

[0029] In step 2, the nucleic acid is dissolved in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to prepare a nucleic acid solution with a concentration of 100 μmol / L, and the nucleic acid sequence is 5′-ACAGAACAACCAACGTCGCTCCGGGTACTTCTTC-3′.

[0030] Step 3, take 1mL of 1mmol / L sodium arsenite solution and dilute it to 10mL, and after standing at 25°C for 1h, measure the conductivity value σ of the sodium arsenite solution 1 Take 20-80 μL of 100 μmol / L nucleic acid solution and dilute it to 10 mL, place it at 25°C for 1 hour, and measure the conductance value σ of the nucleic acid solution 2 Take ...

Embodiment 2

[0037] Step 1, configure 25mmol / L of 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 7.24, weigh 0.1299g of sodium arsenite, and carry out in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution After configuration, a 10 mmol / L sodium arsenite solution was obtained, and then diluted to obtain a 1 mmol / L sodium arsenite solution.

[0038] Step 2, dissolving reduced glutathione in 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to obtain a concentration of 10mmol / L glutathione solution, and passing nitrogen into the glutathione solution to exhaust oxygen After 10 minutes, the glutathione solution after deoxygenation was obtained.

[0039] Step 3, take 1mL of 1mmol / L sodium arsenite solution and dilute it to 10mL, after standing at 25°C for 3h, measure the conductance value σ of the sodium arsenite solution 1 Take 1mL of 10mmol / L glutathione solution and dilute it to 10mL after deoxygenation. After standing at 25°C for 3h, measur...

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Abstract

The invention provides a determining method for the mutual effect of metal ions and ligands. The method is used for determining the combination degree of the metal ions and the ligands. The method ischaracterized by comprising the following steps of S1, preparing a non-ion buffer solution with the concentration being 10-100 mmol / L, adjusting the pH value of the non-ion buffer solution as 6-8, adopting the non-ion buffer solution for configuring a metal ion solution, and obtaining a metal ion solution with the metal ion concentration being 1 mmol / L; S2, dissolving the ligands into the non-ionbuffer solution to prepare a ligand solution with the concentration being 100 micromol / L-100 mmol / L, and mixing the metal ion solution and the ligand solution according to the ratio being 1:1-1:4, andplacing the mixture under the temperature of 25-37 DEG C for 1-4 h, and obtaining a mixed solution; S3, adopting a conductivity meter for measuring the conductivity sigma 0 of the non-ion buffer solution, the conductivity sigma 1 of the metal ion solution, the conductivity sigma 2 of the ligand solution and the conductivity sigma 3 of the mixed solution; S4, according to the combination degree calculation formula, performing calculation, and obtaining the combination degree eta of metal ions and ligands.

Description

technical field [0001] The invention belongs to the technical field of analysis and testing, and in particular relates to a method for measuring the interaction between metal ions and ligands. Background technique [0002] The pollution of heavy metal ions has always been a hazard factor that plagues the environment and food safety. In order to accurately assess the degree of contamination of samples, the use of ion-imprinted polymers to selectively enrich and detect trace amounts of heavy metal ions in samples has become an effective method. means. In the preparation process of ion-imprinted materials, selecting appropriate ligands to combine with template ions through covalent or non-covalent bonds is a key step in the construction of selective recognition cavities. Therefore, selecting ligand molecules with strong binding force can effectively improve the selective enrichment performance of ion imprinted materials. [0003] At present, ligands are screened mainly throug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/06
CPCG01N27/06
Inventor 徐斐叶泰郭猛袁敏曹慧于劲松
Owner UNIV OF SHANGHAI FOR SCI & TECH