Strain of Pediococcus acidilactici of canine origin and application thereof

A technology derived from Pediococcus lactis and canine, applied in the field of microorganisms, to achieve the effect of improving the total antioxidant capacity, strong cell adhesion, and reducing the ratio of material to weight

Active Publication Date: 2019-01-11
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Excellent strains are the basis for the development of canine microecological preparations. Probiotics derived from the host itself have been proven to be safer and more effective. However, currently there are few types o...

Method used

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  • Strain of Pediococcus acidilactici of canine origin and application thereof
  • Strain of Pediococcus acidilactici of canine origin and application thereof
  • Strain of Pediococcus acidilactici of canine origin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation, identification and safety evaluation of Pediococcus lactis R76

[0036] 1. Materials and methods

[0037] 1.1 Materials

[0038] 1.1.1 Sample source and medium preparation

[0039] (1) Sample source: The feces of a healthy unweaned Border Collie puppy were collected in Nanguan District, Changchun City.

[0040] (2) Preparation of MRS liquid medium

[0041]

[0042] Mix the reagent with 1L of distilled water, adjust the pH value to 6.8, and autoclave at 115°C for 15 minutes.

[0043] (3) MRS solid medium: Add 1.5 g of agar powder to every 100 mL of MRS liquid medium, and autoclave at 115° C. for 15 minutes.

[0044] 1.1.2 Experimental animals

[0045] 30 SPF grade BALB / c mice, 18g-22g, were purchased from Beijing Huafukang Company (Certificate No. 11401300071553).

[0046] 1.1.3 Main test reagents

[0047]

[0048]

[0049] 1.2 Method

[0050] 1.2.1 Isolation of lactic acid bacteria from fecal samples

[0051]Collect the feces of a ...

Embodiment 2

[0128] Example 2 Effect of Pediococcus lactis on intestinal morphology, flora and blood indicators of puppies

[0129] 1 Materials and methods

[0130] 1.1 Materials

[0131] 1.1.1 Strains

[0132] Pediococcus lactis R76 deposited in the present disclosure.

[0133] 1.1.2 Experimental animals and management

[0134] 20 weaned healthy puppies (60 days old) were fed with puppies food twice a day (see Table 2-1 for nutritional composition), had free access to drinking water, and cleaned the kennels every day.

[0135] Table 2-1 Nutrient composition of dog food for puppies

[0136]

[0137] 1.1.3 Experimental main reagents

[0138]

[0139] 1.2 Method

[0140] 1.2.1 Effect of Pediococcus lactis on growth performance of puppies

[0141] 20 weaned healthy puppies were randomly divided into groups, and the test group and the PBS buffer control group (grouping and oral dosage were shown in Table 2-2) were set to be fed with Pediococcus lactis preparation, fed once a day, ...

Embodiment 3

[0203] Example 3 Study on the effect of canine-derived Pediococcus lactis on alleviating the stress of transporting puppies

[0204] 1 Materials and methods

[0205] 1.1 Materials

[0206] 1.1.1 Strains

[0207] Pediococcus lactis R76 deposited in the present disclosure.

[0208] 1.1.2 Experimental animals and management

[0209] A pet shop in Changchun purchased a batch of weaned puppies. After long-distance transport by car, 20 of them developed stress reactions, mainly manifested as reduced feed intake and severe diarrhea. The control group was given PBS buffer solution (see Table 3-1 for grouping and oral dosage), fed once a day, and the transport-stressed puppies were fed the same puppy food, the kennels were cleaned every day, and drinking water was free.

[0210] Table 3-1 Puppies grouping and oral dosage form

[0211]

[0212] 1.2 Method

[0213] 1.2.1 Effect of Pediococcus lactis on recovery of feed intake in transport-stressed puppies

[0214] The puppies i...

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Abstract

The present disclosure relates to a strain of Pediococcus acidilactici of canine origin and an application thereof. The strain is classified as Pediococcus acidilactici and numbered JLAPCC-P76, deposited at China Typical Culture Collection on 28 July 2018, Wuhan University, Wuhan, China, with deposit number CCTCC M 2018500. Fresh faeces of puppies are taken as a sample, lactic acid bacteria strains with excellent characteristics are screened out, After feeding weaned puppies for 8 weeks, the intestinal morphology and structure, the bacterial flora and blood indexes are measured, Evaluate its digestion and absorption to puppy dog, intestinal microflora and antioxidant activity, At that same time, in order to evaluate the therapeutic effect of the screened lactic acid bacteria on the stresssymptom of the pet puppies, the present invention gives the stress puppies with anorexia and diarrhea aft long-distance transportation to be fed with lactic acid bacteria, and observes the recovery ofdiarrhea and the recovery of diet, so as to alleviate the common transport stress symptoms in the pet dog trading process.

Description

technical field [0001] The disclosure belongs to the technical field of microorganisms, and in particular relates to a strain of Pediococcus lactis derived from canine and its application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Probiotics are a type of active microorganisms that can produce beneficial effects on the health of the host when the body ingests an appropriate amount. The main strains are Lactobacillus, Bacillus, Bifidobacterium, Enterococcus and Pediococcus. It is made into microecological preparation products to solve some of the problems encountered in animal feeding. At present, the number of registered dogs in my country has reached 70 million, which puts forward a higher demand for healthy dog ​​breeding. Studies have shown that when dogs use probiotics, probiotics can improve the dog's immunity, reduce stress responses...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P1/12A61P1/14C12R1/01
CPCA61K2035/115A61P1/12A61P1/14C12N1/20C12N1/205C12R2001/01
Inventor 王春凤王建忠杨桂连石春卫李宁单宝龙谷魏王红
Owner JILIN AGRICULTURAL UNIV
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