Strain of Pediococcus acidilactici of canine origin and application thereof
A technology derived from Pediococcus lactis and canine, applied in the field of microorganisms, to achieve the effect of improving the total antioxidant capacity, strong cell adhesion, and reducing the ratio of material to weight
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Embodiment 1
[0035] Example 1 Isolation, identification and safety evaluation of Pediococcus lactis R76
[0036] 1. Materials and methods
[0037] 1.1 Materials
[0038] 1.1.1 Sample source and medium preparation
[0039] (1) Sample source: The feces of a healthy unweaned Border Collie puppy were collected in Nanguan District, Changchun City.
[0040] (2) Preparation of MRS liquid medium
[0041]
[0042] Mix the reagent with 1L of distilled water, adjust the pH value to 6.8, and autoclave at 115°C for 15 minutes.
[0043] (3) MRS solid medium: Add 1.5 g of agar powder to every 100 mL of MRS liquid medium, and autoclave at 115° C. for 15 minutes.
[0044] 1.1.2 Experimental animals
[0045] 30 SPF grade BALB / c mice, 18g-22g, were purchased from Beijing Huafukang Company (Certificate No. 11401300071553).
[0046] 1.1.3 Main test reagents
[0047]
[0048]
[0049] 1.2 Method
[0050] 1.2.1 Isolation of lactic acid bacteria from fecal samples
[0051]Collect the feces of a ...
Embodiment 2
[0128] Example 2 Effect of Pediococcus lactis on intestinal morphology, flora and blood indicators of puppies
[0129] 1 Materials and methods
[0130] 1.1 Materials
[0131] 1.1.1 Strains
[0132] Pediococcus lactis R76 deposited in the present disclosure.
[0133] 1.1.2 Experimental animals and management
[0134] 20 weaned healthy puppies (60 days old) were fed with puppies food twice a day (see Table 2-1 for nutritional composition), had free access to drinking water, and cleaned the kennels every day.
[0135] Table 2-1 Nutrient composition of dog food for puppies
[0136]
[0137] 1.1.3 Experimental main reagents
[0138]
[0139] 1.2 Method
[0140] 1.2.1 Effect of Pediococcus lactis on growth performance of puppies
[0141] 20 weaned healthy puppies were randomly divided into groups, and the test group and the PBS buffer control group (grouping and oral dosage were shown in Table 2-2) were set to be fed with Pediococcus lactis preparation, fed once a day, ...
Embodiment 3
[0203] Example 3 Study on the effect of canine-derived Pediococcus lactis on alleviating the stress of transporting puppies
[0204] 1 Materials and methods
[0205] 1.1 Materials
[0206] 1.1.1 Strains
[0207] Pediococcus lactis R76 deposited in the present disclosure.
[0208] 1.1.2 Experimental animals and management
[0209] A pet shop in Changchun purchased a batch of weaned puppies. After long-distance transport by car, 20 of them developed stress reactions, mainly manifested as reduced feed intake and severe diarrhea. The control group was given PBS buffer solution (see Table 3-1 for grouping and oral dosage), fed once a day, and the transport-stressed puppies were fed the same puppy food, the kennels were cleaned every day, and drinking water was free.
[0210] Table 3-1 Puppies grouping and oral dosage form
[0211]
[0212] 1.2 Method
[0213] 1.2.1 Effect of Pediococcus lactis on recovery of feed intake in transport-stressed puppies
[0214] The puppies i...
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