Unlock instant, AI-driven research and patent intelligence for your innovation.

Wheat peptide and fucoidan composition, methods of preparation and use thereof

A technology of fucoidan and composition, which is applied in the field of composition of wheat peptide and fucoidan, its preparation and use, and can solve problems such as reduction and damage

Inactive Publication Date: 2019-01-15
ACCESS BUSINESS GRP INT LLC
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is a need for compositions that can prevent, reduce the risk of, or alleviate GI damage such as gastric mucosa in subjects who have or have such damage risk

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wheat peptide and fucoidan composition, methods of preparation and use thereof
  • Wheat peptide and fucoidan composition, methods of preparation and use thereof
  • Wheat peptide and fucoidan composition, methods of preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1: Wheat peptide, fucoidan and Radix Paeoniae Alba have a synergistic effect on the inhibition of IL-8 production in AGS cells.

[0106] Treatment of human gastric adenocarcinoma with 3μg / mL wheat peptide + 0.3ug / mL fucoidan; 0.3ug / mL white peony; or 3ug / mL wheat peptide + 0.3ug / mL fucoidan + 0.3ug / mL white peony (observation) (AGS) cells. Briefly, 25,000 AGS cells / well (ATCC #CRL-1739) were dispensed in 100 μL of RPMI 1640 medium in 96-well tissue culture-treated microplates and maintained at 37°C, 5% CO 2 Incubate overnight in a tissue culture incubator. Components were prepared in DMSO at 400X final concentrations in the assay. The 400X components were then diluted 1:100 in RPMI 1640 medium to create a 4X stock solution. Then add 50 μL of 4X component stock solution to the microplate containing AGS cells, and keep at 37 °C, 5% CO 2 incubated in a tissue culture incubator. After 4 hours of incubation, prepare 0.04ug / mL TNFa + 0.04ug / mL IL-1b in RPMI1640 m...

Embodiment 2

[0108] Example 2: Wheat peptide, fucoidan and magnolia bark have synergistic effects on the inhibition of IL-8 production in AGS cells.

[0109] Humans were treated with 100 μg / mL wheat peptide+10 μg / mL fucoidan; 10ug / mL magnolia bark; or 100ug / mL wheat peptide+10ug / mL fucoidan+10ug / mL magnolia bark (observation) Gastric adenocarcinoma (AGS) cells. 25,000 AGS cells / well (ATCC #CRL-1739) were seeded in 100 μL RPMI1640 medium in 96-well tissue culture-treated microplates and maintained at 37 °C, 5% CO 2 Incubate overnight in a tissue culture incubator. Components were prepared in DMSO at 400X final concentrations in the assay. The 400X components were then diluted 1:100 in RPMI 1640 medium to create a 4X stock solution. Then add 50 μL of 4X component stock solution to the microplate containing AGS cells, and keep at 37 °C, 5% CO 2 incubated in a tissue culture incubator. After 4 hours of incubation, prepare 0.04ug / mL TNFα + 0.04ug / mL IL-1β in RPMI1640 medium and add 50 μL t...

Embodiment 3

[0111] Example 3: Wheat peptide, fucoidan and Centella asiatica have a synergistic effect on the inhibition of IL-8 production in AGS cells.

[0112] Treated with 200μg / mL wheat peptide + 20μg / mL fucoidan; 20ug / mL Centella asiatica; or 200ug / mL wheat peptide + 20ug / mL fucoidan + 20ug / mL Centella asiatica (observation) to treat human gastric adenocarcinoma ( AGS) cells. Dispense 25,000 AGS cells / well (ATCC #CRL-1739) in 100 μL of RPMI 1640 medium in 96-well tissue culture-treated microplates and maintain at 37 °C, 5% CO 2 Incubate overnight in a tissue culture incubator. Components were prepared in DMSO at 400X final concentrations in the assay. The 400X components were then diluted 1:100 in RPMI 1640 medium to create a 4X stock solution. Then add 50 μL of 4X component stock solution to the microplate containing AGS cells, and keep at 37 °C, 5% CO 2 incubated in a tissue culture incubator. After 4 hours of incubation, prepare 0.04ug / mL TNFa + 0.04ug / mL IL-1b in RPMI1640 me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A composition comprising an effective amount of wheat peptide and an effective amount of fucoidan is described. In addition, an effective amount of an ingredient or extract comprising at least one ofwhite paeony root, magnolia bark, or Centella asiatica in combination with wheat peptide and fucoidan is described. In addition, methods of preparing and using the compositions are described.

Description

Background technique [0001] Wheatpeptide and fucoidan alone or in combination with constituents or extracts of white peony root, Magnolia officinalis bark and / or Asiatic pennywort herb are described Compositions. In addition, methods of preparing the compositions and using the compositions to prevent and / or reduce damage or injury to the gastrointestinal (GI) tract (e.g. gastric mucosa) are described. Risk of harm (by modulating biomarkers) approach. [0002] Injury or damage to the gastric mucosa can be triggered by many factors including, for example, Helicobacter pylori (H. )Wait. These factors often lead to oxidative stress (oxidative stress), in which reactive oxygen species (ROS) and inflammation damage the gastric mucosa, resulting in gastric mucosal damage. [0003] IL-8 is a pro-inflammatory cytokine found to be elevated in humans infected with H. pylori. TNF-α stimulates IL-8 secretion, which is known to contribute to epithelial cell damage. [0004] Prostaglan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/899A61P1/04A61P1/00A61P31/04A61K31/715
CPCA23V2002/00A61K31/715A61K36/23A61K36/575A61K36/65A61K36/71A61K36/899A23L33/00A61K2300/00A23V2250/21A61K9/08A61K9/20A61K9/48A61K31/737A61K36/02A61K38/01A61P1/00A61P1/04A61P31/04
Inventor 桂敏M.胡德C.伯恩斯李敏杰C.胡J.朔尔滕
Owner ACCESS BUSINESS GRP INT LLC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com