In-vitro banana stem tip ultralow-temperature storage method

Abstract

The invention belongs to the technical field of low-temperature storage of bananas and discloses an in-vitro banana stem tip ultralow-temperature storage method comprising the steps: pre-culturing a banana seedling with the height of 3.0-6.0 cm in a culture medium containing 0.4 mol / L of sucrose for 2 d; stripping off a stem tip with 1-2 leaf primordia under the condition of room temperature; loading the stem tip in a loading liquid, then, treating the stem tip by using a vitrification solution, and preparing droplets on an aluminum foil strip; storing the stem tip in liquid nitrogen for at least 1 h, defrosting and washing the stem tip by using 1.2 mol / L of sucrose culture solution, wherein the stem tip is washed for 10 min every time; and transferring the treated stem tip to an MS culture medium containing 0.3 mol / L of sucrose, culturing the stem tip in a dark room for 2 d, then, transferring the stem tip into an MS culture medium containing 0.5 mg / L of 6-BA, culturing the stem tip for 30-35 d, inoculating seed sources recovered to normally grow into a novel recovery growth culture medium for multiplication and rapid propagation to obtain cluster buds, and carrying out culture ina rooting culture medium. Due to the long-term storage in liquid nitrogen, an effective approach is provided for the safety storage of banana germplasm.

Description

technical field [0001] The invention belongs to the technical field of low-temperature preservation of bananas, and in particular relates to a method for ultra-low temperature preservation of isolated banana stem tips. Background technique [0002] Banana is a plant of the genus Musa of the Musa family, which is widely planted in tropical areas. Bananas are fragrant and nutritious. The plant is a large herb, emanating from the rhizome and forming a false stem 3 to 6 meters (10 to 20 feet) high from the lower part of the leaf sheath; the leaves are oblong to oval, and some are as long as 3 to 3.5 meters (10 to 11.5 feet), 65 centimeters (26 inches) wide, with 10 to 20 clustered stem tops. The spikes are drooping, drawn from the top of the false stem, with many flowers, light yellow; the inflorescences are drooping, with 10-20 clusters of fruit, about 50-150. After the plant bears fruit, it dies, and the suckers grown from the rhizome continue to reproduce, and each plant ca...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: at present, the preservation of banana resources in my country mainly adopts field preservation combined with in vitro test-tube seedling preservation, which consumes a lot of manpower, material resources and financial resources.

Moreover, field preservation is easily affected by natural disasters, pests, diseases, etc.; preservation of test-tube seedlings requires continuous subculture, which is prone to pollution loss and germplasm degradation.

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