Quick counting plate for copepod eggs

A copepod and counting board technology, which is applied in the fields of marine biology and tank aquaculture, can solve the problems of easy error and low efficiency, and achieves the effects of high accuracy, convenient operation and avoiding egg damage.

Active Publication Date: 2019-01-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When studying the hatching rate of eggs, related toxicology research and nutrition research, etc., counting eggs is quite difficult to operate because of their tiny size. At present, they are carefully sucked from the breeding water with plastic straws. And microscopic inspection counting, low efficiency, error-prone

Method used

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  • Quick counting plate for copepod eggs
  • Quick counting plate for copepod eggs
  • Quick counting plate for copepod eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Such as Figure 1~6 As shown, the copepod egg rapid counting plate includes a base 1, on which the array of adsorption micropores 6 is arranged, and the bottom of the adsorption microholes 6 is connected with a water outlet pipe 13, and the side of the orifice of the adsorption micropores 6 is provided with The water inlet pipe 3 , the water outlet pipe 13 and the water inlet pipe 3 are respectively connected with the micro electric pump 5 arranged on the base 1 through the flexible hose 4 . The base 1 is engraved with a coordinate system corresponding to the array arrangement of the adsorption micropores 6. The present invention utilizes the micro-electric pump 5 to form a micro-negative pressure to form an adsorption force on a single mature copepod egg, so that it can be quickly positioned in the adsorption micropores. 6 and no threshing, only need to calculate the number of adsorption micropores 6 arranged in a neat square array to calculate the number of mature cop...

Embodiment 2

[0034] During the actual use of the copepod egg rapid counting board of the present invention:

[0035] 1) Before counting, according to the experimental requirements, manually adjust the shielding plate 11 on the top cover 9 to fix the number of eggs, and use the second hinge 701 to make the plane height of the collection tank 7 lower than or make the collection tank 7 stick to the base 1 the back prevents unnecessary copepod ovum from falling into the collection tank 7;

[0036] 2) Start the micro electric pump 5, drive the outlet pipe 13 to drain water, and form a slight negative pressure in the adsorption micropore 6;

[0037] 3) Use a beaker or a wide-mouth pipette to transfer the water body containing eggs, pour the adsorption micropores 6, the negative pressure will adsorb the eggs, and keep a slight negative pressure, and use a magnifying glass to quickly check the adsorption rate of the micropores on the eggs;

[0038] 4) Rinse the surface of the counting plate with ...

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Abstract

The invention provides a quick counting plate for copepod eggs, which comprises a base, wherein adsorption micropores are arranged in array on the base. A water outlet pipes is connected at the bottomof the adsorption micropores, and a water inlet pipe is arranged on the side of the adsorption micropore opening. The water inlet pipe and the water outlet pipe are respectively connected with a miniature electric pump arranged on the base through hoses. The quick counting plate for copepod eggs can quickly and accurately measure the number of mature copepod eggs with no error and no damage of the eggs, reduces the difficulty of counting the mature copepod eggs, and has high efficiency in metering.

Description

technical field [0001] The invention belongs to the technical fields of marine biology and tank aquaculture, and in particular relates to a rapid counting board for copepod eggs. Background technique [0002] Copepoda, belonging to Arthropoda, Crustacea, Copepoda subclass. It is a small crustacean with a body length of less than 3 mm. It lives as a planktonic and parasitic animal, and is distributed in ocean, fresh water or brackish water. Because it is one of the opening baits for most fish larvae, it is of great significance in aquatic ecosystem food webs and aquaculture. Therefore, the study on generational biology of copepods is a very important research topic. The diameter of mature eggs of most copepod species is about 100 microns. When studying the hatching rate of eggs, related toxicology research and nutrition research, counting eggs is quite difficult to operate because of their tiny size. At present, they are carefully sucked from the breeding water with plastic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/95
CPCA01K61/95
Inventor 李继姬王斌叶莹莹张建设
Owner ZHEJIANG OCEAN UNIV
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