A kind of cell culture device and its cell box

Abstract

The invention provides a cell culture device and a cell box thereof. The cell box is in the shape of a cuboid and comprises a bottom cover (1), a plurality of partitions (2) and a top cover (3) stacked in sequence, and the partitions (2 ) has a first pool groove and a first rib on both sides, and the two first pool grooves stacked together form a middle chamber, and the two first ribs stacked in close contact make multiple middle chambers on the same layer Independent of each other; the second tank of the bottom cover (1) is docked with the first tank of the partition (2) to form a bottom chamber, the third tank of the top cover (3) and the first tank of the partition (2) The grooves are butted to form the top chamber, and both the bottom cover (1) and the top cover (3) have two kinds of ribs, one is in close contact with the first rib of the partition (2) over the entire length, and the other is at the end There are gaps. The cell box provided by the invention has high convenience in use, is suitable for manual operation, and can reduce the risk of contamination in the cell culture process, so that a large number of cells can be harvested in one culture.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture device and a cell box thereof. Background technique [0002] In the past ten years, due to the rapid development of the biological products industry, the traditional method of obtaining biological products from animal tissues through biochemical technology has been far from meeting the needs of the market. Large-scale culture of animal cells in vitro to express specific proteins, Monoclonal antibody, interferon and virus vaccine products have become the most common technology at present. [0003] Currently commonly used cell culture devices include conventional cell culture dishes, plates, bottle cultures, spinner bottle cultures, large bioreactors, etc. The above cell culture devices have the following defects: [0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested in a single ...

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Problems solved by technology

[0004] 1) The surface area for cell growth per unit volume is small, the cell growth density is low, and the number of cells harvested in a single culture is small. To obtain a large number of cells requires a large increase in the number of devices and repeated cultures, and repeated cultures require a lot of materials and labor. and time;

[0005] 2) During the cultivation process, the preparation of culture medium, addition of liquid, and harvesting of cells and cultures are all manual operations step by step. There is a lack of a fully enclosed, automated, programmed, continuous and efficient integrated culture system, which greatly reduces the risk of contamination during the culture process. improve;

[0006] 3) The cell factories currently on the market can achieve the purpose of harvesting a large number of cells in one culture, but they take up a lot of space, and the cultivation process requires a large amount of culture medium, trypsin and other reagents. Taking the ten-layer cell factory as an example, the product height reaches 20cm. After filling the culture solution, the weight of the product increases significantly, and manual operation is very difficult. The whole process needs to be assisted by manipulators. The product is large in size and does not fit the laboratory incubator. It is necessary to purchase a special incubator, which will greatly increase equipment investment and consume a lot. Manpower, time, and space limit its widespread use

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