Loading method of biopsy specimens

A biopsy specimen and biopsy technique, applied in the field of biopsy specimens, can solve the problems of specimen loss, easy contamination, and long specimen transfer time, and achieve the effects of saving transfer time, reducing the risk of contamination, and reducing the probability of specimen loss.

Inactive Publication Date: 2019-01-22
大连市妇女儿童医疗中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of genetic diagnosis before precise embryo implantation/genetic screening before precise embryo implantation. The traditional biopsy method takes a long time to transfer specimens, is easy t...

Method used

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  • Loading method of biopsy specimens
  • Loading method of biopsy specimens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: a kind of loading method of biopsy specimen,

[0044] S1: Biopsy Dish Preparation

[0045] Prepare at least one Falcon 1006 dish as an embryo biopsy dish, each biopsy dish can biopsy 1 to 5 embryos, place 1 embryo in a single microdroplet, the volume of each microdroplet is 18 μL, cover it with mineral oil and put it into culture box, and mark each droplet with the same number as the number of the biopsy embryo;

[0046] S2: Biopsy dish preparation

[0047] Prepare at least one Falcon 1006 dish as an embryo biopsy washing dish, add 5 washing microdroplets to each biopsy washing dish, and the volume of each microdroplet is 18 μL;

[0048] S3: Blastomere biopsy

[0049] The laser method is used to punch holes in the zona pellucida, and the fixed needle of the micro-operating system is used to fix the embryos, so that the gap in the zona pellucida is located at the 2 o'clock direction, and a flat biopsy needle with an inner diameter of 30um is used to enter ...

Embodiment 2

[0058] Embodiment 2: a kind of loading method of biopsy specimen,

[0059] S1: Biopsy Dish Preparation

[0060] Prepare at least one Falcon 1006 dish as an embryo biopsy dish. Each biopsy dish can biopsy 1 to 5 embryos. Place 1 embryo in a single microdroplet. The volume of each microdroplet is 20 μL. Cover it with mineral oil and put it into culture. box, and mark each droplet with the same number as the number of the biopsy embryo;

[0061] S2: Biopsy dish preparation

[0062] Prepare at least one Falcon 1006 dish as an embryo biopsy washing dish, add 6 washing microdroplets to each biopsy washing dish, and the volume of each microdroplet is 20 μL;

[0063] S3: Blastomere biopsy

[0064] The laser method is used to punch holes in the zona pellucida, and the microscopic operating system is used to fix the embryos, so that the gap in the zona pellucida is located at 2 o'clock, and a flat biopsy needle with an inner diameter of 33um is used to enter from the gap in the zona ...

Embodiment 3

[0073] Embodiment 3: a kind of loading method of biopsy specimen,

[0074] S1: Biopsy Dish Preparation

[0075] Prepare at least one Falcon 1006 dish as an embryo biopsy dish. Each biopsy dish can biopsy 1 to 5 embryos. Place 1 embryo in a single microdroplet. The volume of each microdroplet is 22 μL. Cover with mineral oil and place in culture box, and mark each droplet with the same number as the number of the biopsy embryo;

[0076] S2: Biopsy dish preparation

[0077] Prepare at least one Falcon 1006 dish as an embryo biopsy washing dish, add 7 washing microdroplets to each biopsy washing dish, and the volume of each microdroplet is 22 μL;

[0078] S3: Blastomere biopsy

[0079] The zona pellucida is perforated by the laser method, and the embryo is fixed with a microscopic operating system fixing needle, so that the gap in the zona pellucida is located at the 2 o'clock direction, and a flat biopsy needle with an inner diameter of 35um is used to enter from the gap in t...

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Abstract

The invention relates to the field of molecular biology, and discloses a loading method of biopsy specimens. The loading method comprises the followings steps: preparing for a biopsy vessel, preparingfor a biopsy washing vessel, carrying out blastomere biopsy, and carrying out blastaea biopsy. Compared with the traditional biopsy method, the method provided by the invention has the advantages that the intermediate transferring link is saved, the transferring time of the biopsy specimens is saved, the pollution of the specimens is reduced, meanwhile, in the specimen transferring process, the visual operation is realized under an inverted microscope, and therefore, the loss probability of the specimens due to the excessively small specimens or the adhering phenomenon is reduced. The embryogenetic materials are obtained safely and effectively for detection, and thus the diagnosis accuracy and effectiveness are improved.

Description

technical field [0001] The invention belongs to the field of molecular biology operations, in particular to a method for loading biopsy specimens. Background technique [0002] The emergence and development of IVF technology has brought hope to countless infertile families. The first-generation test-tube baby is mainly aimed at female infertility; the second-generation test-tube baby is mainly aimed at male infertility; and the third-generation test-tube baby is a revolution and technological breakthrough, which is a new step in human reproductive technology. A great technical contribution has been made. The third-generation test-tube baby can detect whether there are genetic disease genes in the embryo, which can not only avoid fetal deformity and unhealthiness, but also increase the success rate of test-tube babies, thereby helping couples realize their wish for prenatal and postnatal care. [0003] The third-generation test-tube baby is also called genetic diagnosis bef...

Claims

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Application Information

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IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 马超邵小光张振强
Owner 大连市妇女儿童医疗中心
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