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A kind of hatching method of frog animal embryo

An embryonic development and embryo technology, which is applied in the field of frog embryo incubation, can solve the problems of decreased survival rate, reduced hatching rate of frog embryos, and high natural deformity rate.

Active Publication Date: 2021-07-06
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of eggs laid by frogs is astonishing. There are thousands of eggs per generation, or even more than that. Make the reproduction of frogs worse, and maybe only 5-10% survive in the end. Many frogs have become extinct
It has been shown in the literature that the amide herbicides acetochlor, butachlor, and pretilachlor can all reduce the hatching rate of frog embryos to varying degrees, increase their deformity rate, and eventually lead to a decrease in survival rate

Method used

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  • A kind of hatching method of frog animal embryo
  • A kind of hatching method of frog animal embryo
  • A kind of hatching method of frog animal embryo

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0037] The embodiment of the present invention provides a method for obtaining embryos of Xenopus tropicalis and a method for incubating embryos of Xenopus tropicalis, the steps of which are:

[0038] 1. Embryo acquisition method: Select a pair of sexually mature tropical clawed frogs and place them in a breeding box, and use artificial injection of human chorionic gonadotropin (HCG) to induce oviposition. Each Xenopus frog received 2 subcutaneous injections, the first injection of 20 units of HCG, followed by 160 units of HCG at an interval of 24-28 hours. After the second injection, put the tropical clawed frog into the breeding culture box, put the breeding culture box into the constant temperature incubator, and set the light off state, the temperature is 26 ℃, so that the clawed frog can naturally embrace the breeding in the dark and quiet condition. egg. After about 4.5 hours, use a 0.5 ml straw to collect the fertilized eggs, then put them into a 100 ml beaker, use a p...

Embodiment 2

[0043] The embodiment of the present invention provides the incubation situation of embryo incubation at different temperatures, and the specific steps are as follows:

[0044] Three groups of experimental groups were set up, and the three groups of experimental groups incubated Xenopus tropicalis embryos according to the method of Example 1. The difference was that in step 2, when the embryos were incubated, the embryos were placed in a petri dish and then incubated in a constant temperature incubator, and the incubation temperature was respectively set to At 23°C, 26°C, and 29°C, the development, hatching time and hatching rate of tadpoles were observed. The results are shown in Table 1. It can be seen from Table 1 that due to individual differences in embryos, the hatching time and hatching rate of embryos will fluctuate within a dynamic range within an interval. Both lower and higher temperatures affect embryo development, prolong incubation time and reduce hatchability. ...

Embodiment 3

[0048] The embodiment of the present invention provides the incubation situation of embryo incubation under different densities, and the specific steps are as follows:

[0049] Three groups of experimental groups were set up, and the three groups of experimental groups incubated Xenopus tropics embryos according to the method of Example 1. The difference was that in step 2, when the embryos were incubated, 150 eggs, 250 eggs, and 400 eggs were placed in each 50mL culture solution. After being placed in a petri dish, they were incubated in a constant temperature incubator. The incubation density was set to 150, 250, and 400 respectively to detect the development, hatching time, and deformity rate of tadpoles. The results are shown in Table 2. It can be seen from Table 2 that due to individual differences in embryos, the incubation time of embryos will fluctuate within a dynamic range within an interval. When the incubation density is 150 eggs per 50 mL of culture fluid, the deve...

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Abstract

The invention belongs to the field of biotechnology, in particular to a method for hatching frog embryos. The invention provides a method for hatching frog embryos, comprising the following steps: Step 1, obtaining the embryos of frogs; Step 2, placing the embryos in a ratio of 0-300 embryos per 50ml of MBS embryo culture solution. The embryos were placed in a 90mm petri dish and incubated at 24-27°C to form tadpoles. The method for hatching frog animal embryos provided by the present invention has the advantages of simplicity, high efficiency, simple operation, and strong practicability, and can be applied to the field of artificial breeding and environmental ecological restoration, conforming to the harmonious coexistence between man and nature, and can be applied to artificial breeding, environmental ecology Health and other fields have certain application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for hatching frog embryos. Background technique [0002] With the development of modern industrial technology, the problem of environmental pollution has become increasingly prominent, leading to global warming, sharp decline in forest resources, and reduction in biodiversity. The original healthy biological chain structure has been destroyed, and many animals are facing the danger of extinction. At present, harmonious coexistence and harmonious development between man and nature are the main theme of society. Therefore, in addition to controlling environmental pollution, artificial breeding techniques must be used to increase biological production to meet market demand, reduce man-made wanton hunting, and take corresponding effective measures to help restore the biological chain. Amphibians are an important link in the biological chain and play a link between the preceding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 许燕滨赵建彬
Owner GUANGDONG UNIV OF TECH
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