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Pair of primers for amplifying LINC02253 gene and application thereof

A primer pair and primer pair sequence technology, which is applied in the biological field, can solve the problems of unclear accuracy and specificity of the STC2 gene, and no diagnostic criteria are given, achieving good application prospects, saving time, and accurate results

Active Publication Date: 2019-01-25
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the kit does not give specific and detailed diagnostic criteria, and the accuracy and specificity of the STC2 gene in the diagnosis of rectal cancer liver metastases are not clear

Method used

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  • Pair of primers for amplifying LINC02253 gene and application thereof
  • Pair of primers for amplifying LINC02253 gene and application thereof
  • Pair of primers for amplifying LINC02253 gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Specimen collection was approved by the Medical Ethics Committee of Nanjing Medical University.

[0030] Source of tissue samples: The samples came from 90 patients with colorectal cancer who were treated in the General Surgery Department of the First Affiliated Hospital of Nanjing Medical University between February 2015 and February 2016. (2) No radiotherapy, chemotherapy or other anti-tumor treatment before operation.

[0031] The collected information includes the patient's hospital number, name, gender, age, place of birth, contact address, telephone number, family history of cancer, colonoscopy results, important imaging examination data, surgical records, and postoperative pathological diagnosis results. If the initial record is incomplete, it will be supplemented and perfected later with the help of the hospital's electronic medical record system and the archived data in the medical record room. The relevant information that can be disclosed includes age, gende...

Embodiment 2

[0048] We further verified the expression level of the LINC02253 gene in 96 clinical samples obtained. The collection of samples was approved by the Medical Ethics Committee of Nanjing Medical University.

[0049] Source of tissue samples: The samples came from 90 patients with colorectal cancer who were treated in the General Surgery Department of the First Affiliated Hospital of Nanjing Medical University between February 2015 and February 2016. (2) No radiotherapy, chemotherapy or other anti-tumor treatment before operation. The collected information includes the patient's hospital number, name, gender, age, place of birth, contact address, telephone number, family history of cancer, colonoscopy results, important imaging examination data, surgical records, and postoperative pathological diagnosis results. If the initial record is incomplete, it will be supplemented and perfected later with the help of the hospital's electronic medical record system and the archived data in...

Embodiment 3

[0073] Example 3 LINC02253 expression level in TCGA database

[0074] TCGA (The Cancer Genome Atlas) was initiated by the U.S. government to try to map out the genome variation maps of all human cancers by applying genome analysis technology, especially large-scale genome sequencing, and conduct systematic analysis to find all cancer-causing genes. and small mutations of tumor suppressor genes, understand the occurrence and development mechanism of cancer cells, obtain new diagnosis and treatment methods on this basis, and finally outline the whole new "cancer prevention strategy". Jointly conducted by the National Cancer and Oncology Institute (NCI) and the National Human Genome Research Institute (NHGRI), it is expected to cost $100 million.

[0075]Use the Bioconductor / TCGAbiolinks function package to download and preprocess the mRNA expression RNASEqV2 data of the colorectal cancer dataset (COAD READ) from the TCGA database (http: / / tcga-data.nci.nih.gov / tcga / ), (643 tumors...

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Abstract

The invention discloses a pair of primers for amplifying LINC02253 gene and application thereof, which is mainly used for detecting LINC02253 / GAPDH value by real-time fluorescence quantitative PCR oncolonoscopic sample or operation sample, if the ratio>=0. 00018 is positive, (0.00018 is negative. This method is an auxiliary diagnostic tool. Positive patients should be reexamined periodically if the pathological test is negative. At first, that PCR detection method is convenient, simple and suitable for hospital at all levels to popularize and use. The biopsy samples are helpful to determine the lesion and localize the tumor. Therefore, LINC02253 gene has high accuracy, specificity and practicability in the diagnosis of colorectal cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer pair for amplifying LINC02253 gene and application thereof. Background technique [0002] Colorectal cancer is the most common malignant tumor of the digestive tract and one of the most common diseases today. Ten thousand patients died directly or indirectly from colorectal cancer. Although genetic factors are one of the risk factors for colorectal cancer, most colorectal cancers are sporadic and develop as adenoma-tumours over several years. In terms of survival, the 5-year survival rate of patients with stage I can reach more than 90%, while the survival rate of patients with stage IV is only slightly greater than 10%. Endoscopic or blood screening has been shown to be effective in reducing colorectal cancer incidence and mortality. Disease stage in colorectal cancer is the most important prognostic factor. For example, from 2001 to 2007, the 5-year survival rates of p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 袁小琴周倩雯葛盛左思雨
Owner NANJING MEDICAL UNIV
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