Fluorescent quantitative internal reference genes and their primers and applications under salt stress in Changshan, Haizhou

Abstract

The invention discloses a fluorescent quantitative internal reference gene and its primers and applications under the stress of Changshan salt in Haizhou. The internal reference genes are UBC‑E2, RPL and MDH, wherein the gene sequence of the MDH gene is shown in SEQ ID NO.10, and the RPL gene The gene sequence of the UBC‑E2 gene is shown in SEQ ID NO.3, and the gene sequence of the UBC‑E2 gene is shown in SEQ ID NO.12. This application screens out candidate genes with relatively stable expression based on the existing published genes, evaluates their stability through software, discloses the most stable internal reference genes suitable for Haizhou Changshan salt stress conditions, and uses these genes as a basis to design Real-time fluorescence quantitative PCR primers fill the status quo of no internal reference genes under the salt stress conditions of Changshan Mountains in Haizhou, provide strong support for the accurate quantification of related functional genes under the conditions of salt stress in Changshan Mountains in Haizhou, and can improve the stability, repeatability and reliability of research sex.

Description

technical field [0001] The invention belongs to the field of plant molecular biology, and particularly relates to a fluorescent quantitative internal reference gene, primers and applications thereof under salt stress in Changshan, Haizhou. [0002] technical background [0003] Haizhou Changshan (Clerodendrum trichotomum Thunb.), also known as Stinky Sycamore, is a deciduous shrub or small tree under the genus Clerodendmm L. of the Verbenaceae family. , Few pests and diseases, strong adaptability. In addition, the flower shape is peculiar and beautiful, the flowering period is long, the fruit is colorful and the fruiting period is long in autumn and winter. It is an excellent autumn flower and fruit viewing tree species. According to previous experimental results, Haizhou Changshan has very good salinity-tolerance and can be used for greening and restoration of saline-alkali land. However, the research on genes under salt stress in Haizhou Changshan has not been reported yet...

AI Technical Summary

Problems solved by technology

According to the results of previous experiments, Haizhou Changshan has very good salt-alkali tolerance and can be used for afforestation and restoration of saline-alkali land. However, there has been no report on the genetic research of Haizhou Changshan under salt stress.

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