Unlock instant, AI-driven research and patent intelligence for your innovation.

Use of AGAP9 in preparation of product for diagnosis of osteoarthritis

A technology for osteoarthritis and products, applied in the field of disease diagnosis

Inactive Publication Date: 2019-02-01
PEOPLES HOSPITAL OF DEYANG CITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experiment has not yet reached that step in the future, but it is just trying its feasibility with the help of more mature and easier to popularize clinical detection methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of AGAP9 in preparation of product for diagnosis of osteoarthritis
  • Use of AGAP9 in preparation of product for diagnosis of osteoarthritis
  • Use of AGAP9 in preparation of product for diagnosis of osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Screening of differentially expressed genes

[0046] 1. Materials:

[0047] Synovial tissues from 5 OA patients (OA group) were obtained from OA patients undergoing knee arthroplasty or synovectomy. The cases used were in line with the diagnostic criteria for OA proposed by Altam. 3 cases of normal synovial tissue (Nor group) came from joint synovial tissue of patients undergoing trauma surgery. All clinical samples used in this study were informed to the patients and approved by the ethics committee of the hospital.

[0048] The clinical information of the selected subjects is shown in Table 1.

[0049] Table 1 Clinical information

[0050]

[0051]

[0052] 2. Obtaining tissue RNA

[0053] Total RNA was extracted from tissue samples, and the concentration and purity of the extracted RNA were detected by Nanodrop2000, the integrity of RNA was detected by agarose gel electrophoresis, and the RIN value was determined by Agilent2100. A single library...

Embodiment 2

[0071] Example 2 Large sample verification screened out differentially expressed genes

[0072] Based on the results of previous sequencing and according to the size of the P value, we selected the AGAP9 gene for verification.

[0073] 1. Sample collection

[0074] According to the method of Example 1, 42 cases of osteoarthritis synovial tissue and 42 normal synovial tissue were collected.

[0075] 2. Validation at the mRNA level

[0076] 2.1 Extract tissue RNA

[0077] Step is with embodiment 1.

[0078] 2.2 Reverse transcription

[0079] Reverse transcription using Primescript 1 st strand cDNA synthesis kit kit, the operation steps are as follows:

[0080] (1) Add the following reaction liquid in the microcentrifuge tube, as shown in Table 2:

[0081] Table 2 Reaction liquid

[0082]

[0083]

[0084] (2) Incubate at 70°C for 5 minutes, then rapidly cool to 4°C;

[0085] Add the following reaction reagents into the microcentrifuge tube to make a reaction syst...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a diagnostic tool for osteoarthritis. The diagnostic tool can realize the purpose of diagnosis by detecting the AGAP9 gene and its expression product. The study result shows that the mRNA expression level of the AGAP9 gene is significantly up-regulated in patients with osteoarthritis compared with in normal synovial tissue. According to the correlation between the AGAP9 gene and osteoarthritis, a kit for diagnosing osteoarthritis can be prepared, which can be widely used clinically.

Description

technical field [0001] The present invention relates to the field of disease diagnosis, more specifically, the present invention relates to a disease diagnosis method by means of detecting AGAP9 abnormality. Background technique [0002] Osteoarthritis (OA) is a common chronic and progressive joint disease among middle-aged and elderly people. Synovial inflammatory response is the main pathological feature, its pathogenesis is not completely clear so far, and early diagnosis is difficult. The commonly used clinical diagnostic methods are often aimed at middle and late patients. The impact is more direct and obvious. [0003] Osteoarthritis is considered a chronic disease that can remain "silent" for long periods of time, and its early stages are difficult to diagnose clinically; diagnosis is usually based on clinical symptoms and imaging changes. However, X-ray has a poor sensitivity and precision, only provides a cumulative damage, and the error is relatively large, so it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/178G01N33/6893G01N2800/105
Inventor 刘跃洪周宇陈曦王志聪张建军汪红杨灵江伟
Owner PEOPLES HOSPITAL OF DEYANG CITY