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Application of oxyclozanide in preparing anti-streptococcus suis medicine

The technology of hydroxychlorozalamide and Streptococcus suis is applied in the field of microbial infectious diseases and medicine, which can solve the problems of aggravating drug residues and streptococcus drug resistance, and achieves the effect of avoiding drug-resistant strains and preventing Streptococcus suis infection.

Inactive Publication Date: 2019-02-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the irrational use of drugs, the phenomenon of streptococcal drug resistance is also becoming more and more serious, which indirectly aggravates the phenomenon of drug residues.

Method used

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  • Application of oxyclozanide in preparing anti-streptococcus suis medicine
  • Application of oxyclozanide in preparing anti-streptococcus suis medicine
  • Application of oxyclozanide in preparing anti-streptococcus suis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The MIC value of hydroxyclozanide against Streptococcus suis SC19 strain was determined according to the NCCLS antimicrobial susceptibility test operation standard.

[0019] The drug was dissolved in DMSO to prepare a stock solution of 5120 μg / mL, frozen at -20°C, taken out and diluted 10 times with PBS for use. The purified Streptococcus suis SC19 was streak-inoculated on the sheep blood agar plate, cultured at 37°C for 20 h, and the colonies were washed with normal saline by turbidimetric counting method, and the bacterial solution was diluted to a final concentration of 0.5 McFarland turbidity (1.5 ×10 8 CFU / mL), and then use MH+5% liquid medium to dissolve horse blood to dilute the bacterial solution 1:100 for later use (the bacterial solution should be used up within 15 minutes at this time). Add 50 μL of MH + 5% liquid medium for dissolving horse blood into each well of a 96-well culture plate, take 50 μL of the diluted medicinal solution and add it to the first ...

Embodiment 2

[0025] The toxicity of hydroxyclozanide to cells was detected by WST-1 method using monkey-derived vero cells.

[0026] Vero cells were cultured in DMEM containing 10% fetal bovine serum at 37°C, 5% CO 2 cultivated under conditions. Vero cells were cultured in 96-well plates at 70-80% confluency in a volume of 100 μL / well medium. Mix serially diluted compounds with cells at 37°C, 5% CO 2 Incubate for 24 hours, and set up the control wells of pure cells without drugs. After 20 hours of incubation, 10 μL of WST-1 solution was added to each well, and after 4 hours, the reduction of WST-1 was detected by a microplate reader using the absorbance at 490 nm. Calculate the percent fluorescence of drug wells relative to untreated wells, i.e. drug well OD 490 / control well OD 490 , to obtain cell viability. see results figure 1 , Hydroxyclozanide has no significant inhibition on cell growth within the concentration range of MIC value.

Embodiment 3

[0028] In a 96-well plate, add 50 μL of 2% sheep red blood cells suspended in PBS to 50 μL of serially diluted oxyclozanide in PBS and incubate at 37 °C for 1 h. Plates were then centrifuged at 500 g for 5 minutes and 50 μL of supernatant from each well of the assay plate was transferred to a fresh 96-well plate. Cells treated with serially diluted melittin served as a positive control. By visually observing whether there is hemolysis, see figure 2 , it can be seen that the positive control melittin began to cause hemolysis from 2 μg / mL, and hydroxyclozanide did not cause hemolysis of red blood cells at the maximum test concentration of 128 μg / mL, indicating that hydroxyclozanide will not cause physical damage to mammalian cell membranes. destroy.

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Abstract

The invention discloses application of oxyclozanide in preparing an anti-streptococcus suis medicine and belongs to the field of microorganism infectious diseases and medicines. The invention finds that oxyclozanide is capable of inhibiting growth of streptococcus suis, has a protection effect on mice infected by streptococcus suis, and discloses the application in preparing anti-streptococcus suis medicines and preparing medicines for preventing or treating streptococcus suis infections. The invention finds novel medicinal values of the streptococcus suis, and novel prevention and treatment medicines are provided for streptococcus suis infections. Due to adoption of the oxyclozanide, dual purposes of expelling parasites and preventing streptococcus suis infections can be achieved. The oxyclozanide has no cross drug resistance with human antibiotics such as cephalosporin, and thus drug resistance strains of human antibiotics can be avoided.

Description

technical field [0001] The invention relates to the field of microbial infectious diseases and medicine, in particular to the application of hydroxyclozanamide in the preparation of anti-Streptococcus suis medicine. Background technique [0002] Streptococcus suis (S. suis) is a common pathogen that infects pigs and can cause a variety of diseases in the host, including meningitis, arthritis, suppurative lymphadenitis, sepsis, pneumonia, and endocarditis. Pigs of all ages can be affected, among which the septicemia type and meningoencephalitis type are more common in piglets, and the suppurative lymphadenitis type is more common in middle-aged pigs. Since sick pigs, clinically recovered pigs and healthy pigs can carry bacteria, it is not suitable to be noticed. When healthy pigs are introduced into infected pigs, due to mutual contact, the germs can be transmitted through the mouth, nose, and skin wounds, thus leaving hidden dangers. Alternation of seasons or improper feedi...

Claims

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Application Information

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IPC IPC(8): A61K31/609A61P31/04
CPCA61K31/609A61P31/04
Inventor 谭臣鲁浩鲁文嘉陈焕春王湘如王晨晨朱永为王高岩
Owner HUAZHONG AGRI UNIV
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