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Application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs

A technology of saffron and total polysaccharides, applied in anti-inflammatory agents, drug combinations, antipyretics, etc., can solve the problems of resource waste, saffron not fully developed, and unclear mechanism of action, and achieve the effect of inhibiting inflammatory reactions

Active Publication Date: 2021-11-30
ZHEJIANG SHOUXIANGU PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the medicinal function of saffron has not been fully developed, and the mechanism of action is still unclear, resulting in a great waste of resources

Method used

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  • Application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs
  • Application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs
  • Application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Accurately weigh 3 portions of air-dried saffron petal powder after alcohol extraction, 2g each, and put them in 3 similar triangular flasks respectively, add 40mL of distilled water according to the ratio of material to water 1:20, stir and mix well with a glass rod . After sealing with plastic cling film, place it in a water bath at 100°C for extraction for 3 hours. Combine the filtrates filtered through the Buchner funnel and repeat the suction filtration again, then concentrate under reduced pressure in a rotary evaporator to the minimum volume, add ethanol to make the final volume of ethanol reach about 90%, stir and put it in a refrigerator at 4°C overnight. The obtained precipitate was collected by filtering with a 200-mesh nylon filter, and dried in a vacuum oven at 40° C. for 12 hours to obtain the water extract powder of saffron petals.

[0043] Dissolve the water extract powder of saffron petals in ultrapure water, transfer it into a D3000 dialysis bag, clam...

Embodiment 2

[0051] Inhibition of total polysaccharides in petals of saffron can up-regulate the concentration of inflammatory factors induced by LPS

[0052] For convenience, the following saffron petal total polysaccharides are referred to as PPC for short.

[0053] 1. Source of cells

[0054] RAW264.7 mouse-derived monocyte-macrophages were provided by the Institute of Life Medicine, East China Normal University, and commercially available RAW264.7 mouse-derived monocyte-macrophages were also available.

[0055] 2. Cell culture method

[0056] 1) Recovery of RAW264.7 macrophages:

[0057] Take out the frozen cells from the liquid nitrogen tank one day in advance and transfer them to a -80°C ultra-low temperature refrigerator for over-adaptation, add 4mL of cell culture medium to a 60mm cell culture dish, and quickly thaw the cells in a 37°C water bath. When there is only a little bit of ice left, quickly transfer it to a 15mL EP tube pre-added with 5mL of cell culture medium, centrif...

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Abstract

The invention provides the application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs, which belongs to the technical field of application of saffron active substances. The saffron petal total polysaccharides are mixed with pharmaceutically acceptable auxiliary materials to prepare anti-inflammatory drugs. The saffron petals Total polysaccharides achieve anti-inflammation by inhibiting the expression of inflammatory factors. By pre-treating the cells with saffron petal total polysaccharides, the expression of inflammatory factors in the cells caused by lipopolysaccharides can be significantly reduced, thereby inhibiting the inflammatory response.

Description

technical field [0001] The invention belongs to the technical field of application of saffron active substances, and in particular relates to the application of saffron petal total polysaccharides in the preparation of anti-inflammatory drugs. Background technique [0002] Saffron (Saffron, Crocus sativus L.), also known as saffron, saffron, saffron blue, and Safaji, is a perennial herb of the genus Saffron of the family Iridaceae. According to the "Chinese Pharmacopoeia", its medicine Use part is dried style, and its effect of saffron as traditional Chinese medicine is: blood circulation promoting and blood stasis dispelling, cooling blood and detoxification, resolving stagnation and calming the nerves. It is used for amenorrhea in the abdomen, postpartum stasis, febrile rash caused by warm toxin, depression and nausea, palpitation and madness, etc. In addition to medicinal purposes, it can be used as a food additive, food coloring and advanced flavoring. Pharmacological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P29/00C08B37/00
CPCA61K31/715A61P29/00C08B37/0003
Inventor 李振皓李明焱王汉波吴叶锋赵凤杰
Owner ZHEJIANG SHOUXIANGU PHARMA CO LTD
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