Lactobacillus preparation for aquatic products and preparation method thereof

A technology of lactic acid bacteria and Lactococcus lactis, applied in the biological field, can solve the problems of easy death of lactic acid bacteria and difficult to solve storage problems, and achieve the effects of inhibiting colonization and the production of harmful substances, ensuring the health of the body, and improving the survival rate.

Inactive Publication Date: 2019-02-15
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the lactic acid bacteria preparations used in China are liquid preparations. Since lactic acid bacteria are easy to die in liquid, the problem of preservation is difficult to solve, so they have not been promoted and used.

Method used

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  • Lactobacillus preparation for aquatic products and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Lactococcus lactis CECT 539, Lactobacillus aquaticus DSM 21051, and Lactobacillus acidophilus NCIM 2285 were cultivated in the order of slant, liquid seed, and liquid fermentation. The slant and liquid fermentation medium were MRS medium, which consisted of ( W / V): peptone 1%, beef powder 0.5%, yeast powder 0.4%, glucose 2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, add agar powder 1.5-2% to the solid medium, and the pH is about 6.2. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 5%, and cultivate it with aeration at 37°C for 24-48h, so that the bacterial content of each bacteria is ≥10 9 cfu / ml.

[0022] Mix the three kinds of bacterial fermentation liquids by volume percentage 20%: 20%: 30%: 30% to obtain a composite bacterial liquid, and add a freeze-drying protection agent. The formula of the freeze-drying protectio...

Embodiment 2

[0024] Lactococcus lactis CECT 539, Lactobacillus aquaticus DSM 21051, and Lactobacillus acidophilus NCIM 2285 were cultivated in the order of slant, liquid seed, and liquid fermentation. The slant and liquid fermentation medium were MRS medium, which consisted of ( W / V): peptone 1%, beef powder 0.5%, yeast powder 0.4%, glucose 2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, add agar powder 1.5-2% to the solid medium, and the pH is about 6.2. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 5%, and cultivate it with aeration at 37°C for 24-48h, so that the bacterial content of each bacteria is ≥10 9 cfu / ml.

[0025] Mix the three kinds of bacterial fermentation liquids by volume percentage 40%: 30%: 20%: 10% to obtain a composite bacterial liquid, and add a freeze-drying protection agent. The formula of the freeze-drying protectio...

Embodiment 3

[0027] Lactococcus lactis CECT 539, Lactobacillus aquaticus DSM 21051, and Lactobacillus acidophilus NCIM 2285 were cultivated in the order of slant, liquid seed, and liquid fermentation. The slant and liquid fermentation medium were MRS medium, which consisted of ( W / V): peptone 1%, beef powder 0.5%, yeast powder 0.4%, glucose 2%, Tween 800.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02 %, manganese sulfate 0.005%, solid medium by adding agar powder 1.5-2%, pH 6.2 or so. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 5%, and cultivate it with aeration at 37°C for 24-48h, so that the bacterial content of each bacteria is ≥10 9 cfu / ml.

[0028] Mix the three kinds of bacterial fermentation liquids by volume percentage 25%: 25%: 25%: 25% to obtain a composite bacterial liquid, and add a freeze-drying protection agent. The formula of the freeze-drying protection agent incl...

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Abstract

The invention discloses a lactobacillus preparation for aquatic products and a preparation method thereof. The lactobacillus preparation is prepared by performing separate fermentation on a pluralityof lactic acid bacteria isolated from aquatic animal growth environment and intestinal tracts in an appropriate culture medium, stopping culture when the concentration of each strain reaches 107-109CFU/ml, carrying out mixing according to a certain proportion, adding a freeze-drying protective agent, adding trehalose and oligosaccharide, and then performing vacuum freeze drying. The obtained freeze-dried lactobacillus preparation has the characteristics of milk flavor, strong bacterial activity, high bacterial content, long shelf life of 24 months and the like. Supplementing the lactobacilluspreparation to aquatic animals can effectively regulate gastrointestinal flora, promote proliferation of beneficial bacteria, improve immunity, help digestion, inhibit reproduction of harmful bacteria, etc. In addition, the preparation has a function of regulating water quality when used in aquaculture water bodies.

Description

Technical field [0001] The invention discloses a lactic acid bacteria preparation for aquatic products and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] With the rapid development of my country's aquaculture industry, the intensive and large-scale production mode has led to deteriorating aquaculture environment and frequent disease outbreaks, which have affected the growth of aquaculture production and the sustainable development of the aquaculture industry. As a traditional feed additive, antibiotics have played an important role in inhibiting the reproduction of pathogenic microorganisms and reducing bacterial metabolic toxins. However, the long-term use and abuse of antibiotics lead to the imbalance of normal flora in the gastrointestinal tract of animals, adverse effects on the immunity of animals, and side effects such as drug resistance and drug residues, which have brought safety hazards to the breeding industry a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K50/80A23K10/16C12R1/01C12R1/225C12R1/23
CPCA23K10/16A23K50/80C12N1/04C12N1/20
Inventor 储卫华周淑鑫
Owner CHINA PHARM UNIV
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