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Application of nod1/2 in the preparation of drugs for improving the sensitivity of tumor cells to chemotherapeutic drugs

A chemotherapeutic drug and sensitivity technology, applied in the field of biomedicine, can solve the problems of chemotherapeutic drug resistance, toxic and side effects of the body, unsatisfactory liver cancer effect, etc., and achieve environment-friendly, reasonable composition, safe and reliable use Effect

Active Publication Date: 2020-06-23
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, liver cancer cells are prone to drug resistance to chemotherapeutic drugs, and anticancer drugs and chemotherapeutic drugs themselves may cause relatively large toxic and side effects on the body, so the effect of chemotherapy for liver cancer is still unsatisfactory.

Method used

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  • Application of nod1/2 in the preparation of drugs for improving the sensitivity of tumor cells to chemotherapeutic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 NOD1 / 2 improves the chemosensitivity of liver cancer cells

[0026] NOD1 significantly improved the chemosensitivity of liver cancer cell lines: the liver cancer cell line HepG2 was seeded in a 96-well plate, and after overnight attachment, myc-NOD1 was exogenously transferred, and different concentration gradients of 5-FM were added during the transfection medium change , 72 hours after adding the drug, the cell viability was detected by CCK-8 method; the liver cancer cell line HepG2 was seeded in a 96-well plate, after adhering to the wall overnight, exogenously transferred into myc-NOD1, and 5-FM ( 10 μg / ml), after adding the drug for 48h, the cell viability was detected by CCK-8 method; the analysis found that NOD1 can effectively improve the sensitivity of liver cancer cells to 5-FM ( figure 1A-B), indicating that NOD1 can significantly improve the chemosensitivity of liver cancer cells to 5-FM. Liver cancer cell line HepG2 was seeded in a 6-well plate, ...

Embodiment 2

[0029] Example 2 NOD1 improves the chemosensitivity of liver cancer cells in a nude mouse model

[0030] NOD1 significantly improved the chemosensitivity of liver cancer cell lines: 20 male nude mice were applied for about 4 weeks, and the liver cancer cell line HepG2 (1*10^7) was subcutaneously injected into the left and right axillary sides of the nude mice. After the tumor was visible to the naked eye, the myc-NOD1 plasmid (20 μg) was injected into the left tumor, and at the same time, the same amount of myc-vector (20 μg) was given to the right side, and injected once every other day; myc-NOD1 and myc- Vector was divided into two groups, one group was injected with 5-FM (10mg / kg), and the other group was injected with the same volume of PBS, once every other day. 26 days after the first injection of the plasmid, the nude mice were sacrificed and the tumor tissues were stripped, weighed and measured for the length and diameter of the tumor. The tumor growth curve of nude m...

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to an application of NOD1 / 2 in preparing medicament for improving the sensitivity of tumor cells to chemotherapeuticmedicine. The invention finds that the NOD1 and the NOD2 both can improve the chemosensitivity of hepatoma carcinoma cells for the first time. The NOD1 increases the chemosensitivity of the hepatomocarcinoma cells by inhibiting a p-SRC-MAPK signal axis, and the inhibitory effect of the NOD1 on the hepatomo carcinoma cells has a certain synergistic effect with a 5-FM. The NOD2 enhances the chemosensitivity of the hepatomo carcinoma cells by activating a AMPK signal pathway, and the inhibitory effect of the NOD2 on the hepatomo carcinoma cells has the certain synergistic effect with the 5-FM.The application of the NOD1 / 2 gene in improving the chemosensitivity of the hepatoma carcinoma cells obviously improves the chemotherapy result and can reduce side effects caused by the chemotherapy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of NOD1 / 2 in the preparation of drugs for improving the sensitivity of tumor cells to chemotherapeutic drugs. Background technique [0002] Hepatocellular carcinoma (hepatocellμlarcarcinoma, HCC, hereinafter referred to as liver cancer) is a malignant tumor disease that seriously threatens the life and health of our people. Worldwide, liver cancer has become the third leading cause of cancer-related death. In recent years, imaging diagnostic techniques and surgical techniques related to liver cancer have been continuously improved. Targeted therapy drugs for liver cancer represented by sorafenib have also been approved by the US Food and Drug Administration. These achievements provide more and more effective options for the diagnosis and treatment of liver cancer. But unfortunately, looking at the current clinical treatment of liver cancer, the five-year s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/513A61P35/00
CPCA61K31/513A61K48/005A61P35/00
Inventor 韩丽辉马小敏李涛赵云雪邱驭旻朱礼慧林月轲马大鹏秦振志孙偲瑜沈雪城
Owner SHANDONG UNIV
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