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Method of extracting Polygonatum polysaccharides

A Polygonatum polysaccharide and extraction method technology, applied in the field of polysaccharide extraction, can solve the problems of low yield and low extraction efficiency, and achieve the effect of high extraction rate

Inactive Publication Date: 2019-02-19
常馨尹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction efficiency is not high and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The extraction method of polysaccharide Polygonatum comprises the following steps:

[0017] (1) Extract the crude polysaccharide extract: Dry Polygonatum in vacuum at 40°C for 5 hours, crush it through an 80-mesh sieve, then mix it with water according to the ratio of solid to liquid: 1g: 40mL, extract at 70°C for 40min, and then use a power of 300W , Ultrasonic extraction at a frequency of 45KHz for 30min, centrifugation, the supernatant obtained by centrifugation was rotated at 50°C for 80min, then added to an ethanol solution for stirring, left to stand for 12h, filtered, and the resulting precipitate was freeze-dried to obtain polysaccharides Crude extract; Wherein, the mass concentration of described ethanol solution is 95%;

[0018] (2) Extraction: Add the crude polysaccharide extract into the chloroform-n-butanol mixture according to the material ratio of 1g:10mL, the volume ratio of chloroform and n-butanol in the chloroform-n-butanol mixture is 1:3, repeat Ext...

Embodiment 2

[0021] The extraction method of polysaccharide Polygonatum comprises the following steps:

[0022] (1) Extraction of crude polysaccharide extract: Dry Polygonatum in vacuum at 50°C for 3 hours, crush it through an 80-mesh sieve, then mix it with water according to the ratio of solid to liquid: 1g: 40mL, extract at 80°C for 30min, and then use a power of 300 Ultrasonic extraction at -400W, frequency 45KHz for 40min, centrifugation, the supernatant obtained by centrifugation was rotated and evaporated at 60°C for 60min, then added to ethanol solution for stirring, left to stand for 24h, filtered, and the obtained precipitate was freeze-dried. Obtain polysaccharide crude extract; Wherein, the mass concentration of described ethanol solution is 95%;

[0023] (2) Extraction: Add the crude polysaccharide extract into the chloroform-n-butanol mixture according to the material ratio of 1g:10mL, the volume ratio of chloroform and n-butanol in the chloroform-n-butanol mixture is 1:3, re...

Embodiment 3

[0026] The extraction method of polysaccharide Polygonatum comprises the following steps:

[0027] (1) Extract the crude polysaccharide extract: Dry Polygonatum in vacuum for 4 hours at 48°C, crush it through an 80-mesh sieve, then mix it with water according to the ratio of solid to liquid: 1g: 40mL, extract at 75°C for 35min, and then use a power of 300 -400W, frequency 45KHz under the conditions of ultrasonic extraction for 35min, centrifuged, the supernatant obtained by centrifugation was rotated and evaporated at 55°C for 70min, then added to ethanol solution and stirred, allowed to stand for 18h, filtered, and the resulting precipitate was freeze-dried. Obtain polysaccharide crude extract; Wherein, the mass concentration of described ethanol solution is 95%;

[0028] (2) Extraction: Add the crude polysaccharide extract into the chloroform-n-butanol mixture according to the material ratio of 1g:10mL, the volume ratio of chloroform and n-butanol in the chloroform-n-butanol...

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Abstract

The invention provides a method of extracting Polygonatum polysaccharides, comprising the steps of (1) extracting polysaccharide crude extract, to be specific, drying Polygonatum rhizome in vacuum, crushing, mixing with water, soaking for extracting at 70-80 DEG C for 30-40 min, performing ultrasonic extraction, centrifuging to obtain supernate, subjecting the supernate to rotary evaporation, adding into ethanol solution, stirring, allowing standing, filtering, and lyophilizing precipitate to obtain polysaccharide crude extract; (2) extracting, to be specific, adding the polysaccharide crude extract to chloroform-1-butanol mixed liquid in the material ratio of 1 g:10 mL, extracting repeatedly three times, combining triple extracts to obtain an aqueous phase, adding ethanol solution into the aqueous phase until the mass concentration of ethanol reaches 80%, allowing standing for 12-23 hours, filtering, washing obtained solid, and drying by baking to obtain Polygonatum polysaccharides. The method provided herein provides high polysaccharide extraction rate, up to about 15%.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting Polygonatum Polysaccharide. Background technique [0002] Polygonatum Polygonatum is the dried rhizome of Polygonatum Polygonatum, Polygonatum Polygonatum or Polygonatum Polyflora, which is a traditional Chinese medicine in my country and belongs to Chinese herbal medicine with the same origin of medicine and food. This taste is sweet and flat, and has the effects of nourishing the middle and Qi, invigorating the spleen and moistening the lungs, nourishing the kidneys and strengthening the bones. It mainly contains polygonatum polysaccharides, saponins, niacin, quinones, amino acids and trace elements, etc. It has various pharmacological effects such as delaying aging, enhancing immune function, regulating blood lipids, lowering blood sugar, and anti-tumor. Among them, polysaccharide is the main active ingredient, which has s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 常馨尹
Owner 常馨尹