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Lactobacillus plantarum and application thereof in preparation of miscanthus sinensis silage

A technology of Lactobacillus plantarum and miscanthus, which is applied in the field of microorganisms, can solve the problems of high breeding cost, difficulty in silage of miscanthus, and low digestibility, and achieve the effects of improving sensory quality, palatability, and increasing organic acid content

Inactive Publication Date: 2019-02-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a kind of Lactobacillus plantarum and its application in the preparation of miscanthus silage, so as to solve the problems of miscanthus silage difficulty, low digestibility and high breeding cost in the prior art

Method used

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  • Lactobacillus plantarum and application thereof in preparation of miscanthus sinensis silage
  • Lactobacillus plantarum and application thereof in preparation of miscanthus sinensis silage
  • Lactobacillus plantarum and application thereof in preparation of miscanthus sinensis silage

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Experimental program
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Effect test

Embodiment 1

[0019] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0020] The Miscanthus species used for silage preparation in the following examples is Nandi, which can be obtained from the variety owner and can only be used to repeat the experiment of the present invention.

[0021] (1) Rapid preparation of Lactobacillus mixed bacterial agent

[0022] 1) MRS medium (1L)

[0023] Liquid: peptone 10g, yeast extract powder 5g, K 2 HPO 4 2g, diammonium hydrogen citrate 2g, sodium acetate 5g, glucose 20g, beef extract 10g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·H 2 O 0.25g, Tween 80 1ml;

[0024] Solid: Agar 15g CaCO 3 10g

[0025] Preparation method: Add the above ingredients into a triangular flask, add 1L of water, and stir and dissolve on a magnetic stirrer to make a liquid medium; if you want to prepare a solid medium, add 1.5% agar and stir until completely dissolved. Wrap 1% calcium carbonate in newspa...

Embodiment 2

[0038] The microbial bacterial agent added in this embodiment is a mixed solution of Lactobacillus plantarum EC018 and Lactobacillus casei, and the concentration is 10 9 cfu / mL is defined as the MIX group, and the rest of the silage methods are the same as in Example 1.

[0039] The silage prepared from miscanthus as raw material of each embodiment is evaluated:

[0040] 1. Sensory evaluation

[0041] Table 1 Sensory evaluation of silage materials

[0042]

[0043] It can be seen from the above table that the microbial agent provided by the present invention can effectively improve the sensory image of Miscanthus fermented, and greatly improve the appearance and presentation of Miscanthus fodder after silage.

[0044] 2. Comparison of silage quality

[0045] Table 2 Comparison of Miscanthus silage quality under different microbial additives

[0046]

[0047]

[0048]It can be seen from the above table that the present invention uses the Lactobacillus plantarum EC0...

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Abstract

The invention discloses lactobacillus plantarum and application thereof in preparation of miscanthus sinensis silage, and belongs to the field of microorganisms. The lactobacillus plantarum EC018 is obtained by conducting heavy ion mutagenesis on ordinary lactobacillus plantarum and reserved in Chinese common microbe bacterial preservation administration center on October 8th, 2018, and the preservation number is CGMCC NO. 16556. The lactobacillus plantarum EC018 and lactobacillus casei are mixed according to the bacterial population ratio of 1:1 and sprayed on surfaces of miscanthus sinensissegments so that the pH value of the miscanthus sinensis silage can be reduced, the content of soluble carbohydrates and the content of proteins in the miscanthus sinensis silage after fermentation are increased, the ammoniacal nitrogen content is reduced, degradation of the proteins in the miscanthus sinensis silage is avoided, the content of neutral detergent fibers and the content of acid detergent fibers in the miscanthus sinensis silage are reduced, the nutrition quality and feeding value of the miscanthus sinensis silage are improved; meanwhile, the content of organic acid in the miscanthus sinensis silage is increased, the palatability of the miscanthus sinensis silage is improved, and thus miscanthus sinensis becomes available silage.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a plant lactobacillus and its application in the preparation of Miscanthus silage. Background technique [0002] Miscanthus is a perennial C4 plant with the advantages of fast growth, high photosynthetic efficiency, high biomass, low water and fertilizer requirements, and few pests and diseases. Nandi is a unique miscanthus species in my country. It not only has all the excellent qualities of miscanthus, but also its young stems and leaves have high nutritional value, contain high concentrations of water-soluble carbohydrates and crude protein, and the total effective nutrient content is as high as 55%. %, very suitable for development as silage. At present, the lack of high-quality forage resources in my country has become a bottleneck restricting the development of animal husbandry. In order to speed up the development of animal husbandry, it has become an urgent task ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00A23K30/18C12R1/25
CPCA23K30/18C12N13/00C12N15/01C12R2001/25C12N1/205A23V2400/169
Inventor 周功克贺郭胡瑞波何康
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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