Construction method and detection method of UPLC characteristic chromatogram of lophatherum gracile medicinal material

A construction method and a feature map technology, applied in the field of medicine, can solve the problems of inability to reflect the basic characteristics of traditional Chinese medicine decoction materials, and it is difficult to fully reflect the quality characteristics of pale bamboo leaves, so as to achieve good medicinal material quality, overcome durability deviation, and ensure the effect of quality.

Active Publication Date: 2019-02-22
GUANGDONG YIFANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the establishment of the characteristic map of the leaves of the light bamboo reported in the literature is only for the raw materials of the medicinal materials, and the index components are mostly fat-soluble components as the research objects, which are mainly used for the qualitative identification of the authenticity, place of origin, and quality differences of the traditional Chinese medicinal materials, which is difficult to fully reflect The quality characteristics of light bamboo leaves cannot reflect the material basis characteristics of traditional Chinese medicine decoctions

Method used

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  • Construction method and detection method of UPLC characteristic chromatogram of lophatherum gracile medicinal material
  • Construction method and detection method of UPLC characteristic chromatogram of lophatherum gracile medicinal material
  • Construction method and detection method of UPLC characteristic chromatogram of lophatherum gracile medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 The construction of the characteristic map of bamboo leaves medicinal material

[0063] 1. Instruments, reagents and reagents

[0064] The instruments are shown in Table 1, the reagents are shown in Table 2, and the reagents are shown in Table 3:

[0065] Table 1

[0066]

[0067] Table 2

[0068]

[0069] table 3

[0070]

[0071]

[0072] 2. Research object

[0073] In this embodiment, the medicinal materials of dandelion leaves come from 17 batches of samples from 7 authentic or major production areas with large dandelion leaf production in the country, as shown in Table 4 for details:

[0074] Table 4

[0075]

[0076] Remarks: The content of isoorientin is determined according to the content determination method under the item of "Hong Kong Chinese Medicinal Materia Medica" under the item of Elephant Bamboo.

[0077] 3. Preparation of reference substance reference solution, reference medicinal material solution and standard decoction ...

Embodiment 2

[0157] The detection method of embodiment 2 light bamboo leaf medical material

[0158] The present embodiment provides a kind of detection method of light bamboo leaf medical material, and the steps are as follows:

[0159] (1) Chromatographic conditions

[0160] With 4.1 in embodiment 1.

[0161] (2) Preparation of reference substance reference substance solution

[0162] Same as 3 in Example 1.

[0163] (3) Preparation of the sample solution to be tested

[0164] Take 1.0g of the sample to be tested (batch number: DZY18, qualified according to the Chinese Pharmacopoeia), put it in a 50mL volumetric flask, add an appropriate amount of 60% (volume fraction) ethanol aqueous solution, ultrasonically treat (power 500W, frequency 40kHz) for 30 minutes, and extract solution, the extract was allowed to cool, and the volume was fixed to the mark with 60% (volume fraction) ethanol aqueous solution, shaken up, filtered, and the subsequent filtrate was taken as the sample solution to...

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Abstract

The invention relates to a construction method and a detection method of a UPLC characteristic chromatogram of a lophatherum gracile medicinal material. The construction method of the characteristic chromatogram comprises the following steps: preparing a reference substance solution of control samples from isoorientin and swertiajaponin as the control samples; preparing a reference substance solution of a control medicinal material from a lophatherum gracile control medicinal material; taking a lophatherum gracile medicinal material and a standard decoction sample respectively, adding an extraction solvent for extraction, carrying out filtering, and taking the obtained filtrate as a test sample solution and a standard decoction test sample solution; sucking the reference substance solutionof the control samples, the test sample solution and the standard decoction test sample solution respectively, and injecting the solutions into an ultra-high performance liquid chromatograph for measurement; and comparing the obtained test sample chromatogram with a standard decoction test sample chromatogram and calibrating characteristic peaks of four water-soluble components to obtain the UPLCcharacteristic chromatogram of the lophatherum gracile medicinal material. The characteristic chromatogram can be used for qualitative and quantitative analysis of the quality of the lophatherum gracile medicinal material, can ensure the quality of a lophatherum gracile traditional decoction prepared from the medicinal material, and is also suitable for detection of other preparations containinglophatherum gracile.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing and detecting a UPLC characteristic spectrum of a medicinal material of phlox verticillum. Background technique [0002] Bamboo leaves are the dry stems and leaves of grasses Lophatherum gracile Brongn. In summer, it is harvested before flowering and spiked, and dried in the sun. It has the effects of clearing heat and purging fire, relieving restlessness and quenching thirst, diuresis and treating stranguria. Bamboo leaves were first recorded in "Southern Yunnan Materia Medica", and Li Shizhen in the Ming Dynasty said in "Compendium of Materia Medica" that it is sweet, cold, non-toxic, relieves restlessness and heat, facilitates urination, and clears the heart. At present, the known chemical constituents of the leaves of Danzhu are mainly flavonoids, in addition to volatile oils, triterpenoids, phenolic acids, polysaccharides, amino acids, etc. Mode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 王闽予谭吉娇彭致铖张文芳梁浩明胡梅陈向东
Owner GUANGDONG YIFANG PHARMA
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