Construction method and detection method of UPLC characteristic chromatogram of lophatherum gracile medicinal material

Abstract

The invention relates to a construction method and a detection method of a UPLC characteristic chromatogram of a lophatherum gracile medicinal material. The construction method of the characteristic chromatogram comprises the following steps: preparing a reference substance solution of control samples from isoorientin and swertiajaponin as the control samples; preparing a reference substance solution of a control medicinal material from a lophatherum gracile control medicinal material; taking a lophatherum gracile medicinal material and a standard decoction sample respectively, adding an extraction solvent for extraction, carrying out filtering, and taking the obtained filtrate as a test sample solution and a standard decoction test sample solution; sucking the reference substance solutionof the control samples, the test sample solution and the standard decoction test sample solution respectively, and injecting the solutions into an ultra-high performance liquid chromatograph for measurement; and comparing the obtained test sample chromatogram with a standard decoction test sample chromatogram and calibrating characteristic peaks of four water-soluble components to obtain the UPLCcharacteristic chromatogram of the lophatherum gracile medicinal material. The characteristic chromatogram can be used for qualitative and quantitative analysis of the quality of the lophatherum gracile medicinal material, can ensure the quality of a lophatherum gracile traditional decoction prepared from the medicinal material, and is also suitable for detection of other preparations containinglophatherum gracile.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing and detecting a UPLC characteristic spectrum of a medicinal material of phlox verticillum. Background technique [0002] Bamboo leaves are the dry stems and leaves of grasses Lophatherum gracile Brongn. In summer, it is harvested before flowering and spiked, and dried in the sun. It has the effects of clearing heat and purging fire, relieving restlessness and quenching thirst, diuresis and treating stranguria. Bamboo leaves were first recorded in "Southern Yunnan Materia Medica", and Li Shizhen in the Ming Dynasty said in "Compendium of Materia Medica" that it is sweet, cold, non-toxic, relieves restlessness and heat, facilitates urination, and clears the heart. At present, the known chemical constituents of the leaves of Danzhu are mainly flavonoids, in addition to volatile oils, triterpenoids, phenolic acids, polysaccharides, amino acids, etc. Mode...

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Problems solved by technology

At present, the establishment of the characteristic map of the leaves of the light bamboo reported in the literature is only for the raw materials of the medicinal materials, and the index components are mostly fat-soluble components as the research objects, which are mainly used for the qualitative identification of the authenticity, place of origin, and quality differences of the traditional Chinese medicinal materials, which is difficult to fully reflect The quality characteristics of light bamboo leaves cannot reflect the material basis characteristics of traditional Chinese medicine decoctions

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