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Cancer promotion gene JCAD and application by taking the same as target to prepare drugs for treating liver cancer

A technology for the treatment of liver cancer and oncogenes, applied in gene therapy, drug combination, genetic engineering, etc., can solve the problems of initiation, progression and metastasis, and the mechanism of action has not been reported, and achieve the effect of high interference efficiency

Inactive Publication Date: 2019-02-26
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Studies have shown that the liver cancer-related gene JCAD is highly expressed in liver cancer tissues and cell lines, but its influence on the initiation, progression and metastasis of HCC and its mechanism have not been reported so far.

Method used

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  • Cancer promotion gene JCAD and application by taking the same as target to prepare drugs for treating liver cancer
  • Cancer promotion gene JCAD and application by taking the same as target to prepare drugs for treating liver cancer
  • Cancer promotion gene JCAD and application by taking the same as target to prepare drugs for treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Silencing the expression of JCAD inhibits the proliferation of liver cancer cells, and the effect of high expression of JCAD on the proliferation of liver cancer cells

[0033] 1 Cell culture, transfection and infection

[0034] 1.1 Cell culture

[0035] Human liver cancer cells Huh-7, Huh-7-Trans, HLE, HLF and SMCC-7721 were cultured in DMEM medium with 10% fetal bovine serum at 37°C, 5% CO2, and saturated humidity in a cell culture incubator;

[0036] 1.2 Cell transfection and collection of lentivirus

[0037] 1.2.1 The day before transfection, it will be in the logarithmic growth phase of 2~3×10 5 Huh-7, Huh-7-Trans, HLE, HLF and SMCC-7721 cells were seeded on a 6-well plate, cultured in 2 mL of DMEM medium containing 10% fetal bovine serum (without antibiotics), and the cell concentration reached 70- 80%;

[0038] 1.2.2 1 hour before transfection, replace the serum-free Opti-MEM culture medium and culture at 37°C with 5% CO2;

[0039] 1.2.3 Lentiviral plasmids ...

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Abstract

The invention belongs to the technical field of biological medicines, and specifically relates to a cancer promotion gene JCAD and an application by taking the same as a target to prepare drugs for treating liver cancer. Through the proving of tests, The latent cancer promotion gene JCAD can be highly expressed in an HCC cell line and clinical sample, and can interact with LATS2 and inhibit LATS2phosphorylation YAP during the occurrence of cancer, so that the phosphorylation level of the latter can be lowered, and the propagation of liver cancer cells can be promoted through a Hippo signal pathway. An shRNA sequence having an interference effect is also disclosed; and the sequence can disturb the expression of the JCAD in cells. Recombinant lentivirus-mediated JCAD overexpression and corresponding JCAD overexpressing cell lines are also included. The cancer promotion gene JCAD is helpful for the pathogenesis research of liver cancer diseases, and can be taken as a new target to prepare drugs for treating liver cancer.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, relates to the oncogene JCAD and its use, in particular to the oncogene JCAD and its use as a target in the preparation of drugs for treating liver cancer. The cancer-promoting gene JCAD of the present invention contributes to the research on the pathogenesis of liver cancer, and can provide important reference data for the further development of drugs against liver cancer. Background technique [0002] Statistics show that hepatocellular carcinoma (HCC) is the fifth most common malignant tumor in the world and the third leading cause of cancer-related death worldwide. According to statistics, about 700,000 people die of cancer every year in the world, half of them are in China. Clinical studies have shown that the prognosis of HCC is poor, and the 5-year survival rate is less than 15%. Among them, most patients have lost the chance of radical surgery when they are diagnosed, and che...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61P35/00C12N15/867C12N7/01C12N15/113
CPCA61K45/00A61K48/0008A61K48/005C12N7/00C12N15/113C12N15/86C12N2310/14C12N2740/15021C12N2740/15043
Inventor 吴健叶娟
Owner FUDAN UNIV
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