Nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide and application thereof

A technology for lowering blood lipids and yellow croaker, which is applied to applications, medical preparations containing active ingredients, peptides, etc., can solve the problems of limited economic value, low added value of fish snails, and restrictions on the sale of swim bladders, etc., to achieve convenient operation and design reasonable effect

Active Publication Date: 2019-03-01
中科利君股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of fish snails in the market is mainly for cooking and dry product processing. The added value of dried fish snails is low, and has obvious regional characteristics, mainly concentrated in coastal areas, but rarely eaten in the interior. This restricts the sale of swim bladder and its economic value is limited, so it is urgent to develop an economical and feasible deep processing technology for fish snails
However, at present, the process research on the preparation of blood lipid-lowering peptides from yellow croaker swim bladder by using enzymatic hydrolysis technology is in a blank stage.

Method used

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  • Nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide and application thereof
  • Nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide and application thereof
  • Nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A blood lipid-lowering hexapeptide derived from the swim bladder of yellow croaker, a peptide consisting of the amino acid sequence of SEQ ID NO: 1, the amino acid sequence of which is Trp-Gly-Ile-Glu-Ala-Leu (WGIEAL).

[0036] A preparation method of a blood lipid-lowering hexapeptide derived from the swim bladder of yellow croaker, comprising:

[0037] 1) Enzymatic hydrolysis of the swim bladder of yellow croaker: Take fresh Japanese croaker (Nibea japonica), remove the swim bladder, wash and homogenate, add distilled water according to the ratio of solid to liquid 1g:12mL, adjust the pH value to 9.5, add the weight of the swim bladder 2.0% alkaline protease (enzyme activity ≥ 2.0×10 5U / g), enzymolysis at 55°C for 5 hours, inactivating the enzyme at 95°C for 15 minutes, cooling to room temperature, centrifuging at 12,000 r / min for 25 minutes, collecting the supernatant, i.e. enzymatic hydrolyzate of yellow croaker swim bladder;

[0038] 2) Preparation of blood lipid-...

Embodiment 2

[0043] In order to increase the yield of blood lipid-lowering hexapeptide, an optimized method for the preparation of blood lipid-lowering hexapeptide derived from the swim bladder of yellow croaker is as follows:

[0044] The swim bladder homogenization of yellow croaker adopts intermittent ultra-high pressure homogenization. The specific method is: homogenize the washed swim bladder for 30s under the pressure of 168MPa, and then keep the pressure for 60s; repeat the same high-pressure treatment twice after the pressure is released. , that is, three cycles. There is an obvious interaction between ultra-high pressure pressure, homogenization time, and pressure holding time, and there is a quadratic nonlinear relationship with homogenization rate, energy consumption for homogenization, and homogenization liquid. The intermittent ultra-high pressure homogenization in this embodiment Slurry can severely damage the integrity of the swim bladder, and then release the contents of th...

Embodiment 3

[0057] A reagent for down-regulating the expression level of an adipogenic gene, comprising the above-mentioned blood lipid-lowering hexapeptide as an active ingredient.

[0058] A medicament for promoting fatty acid β-oxidation, comprising the above-mentioned blood lipid-lowering hexapeptide as an active ingredient.

[0059] The application of a blood lipid-lowering hexapeptide derived from the swim bladder of yellow croaker is used in the preparation of blood fat-lowering medicines and / or health care products and / or food additives.

[0060] Effects on experimental hyperlipidemia in mice:

[0061] 30 mice, both male and female, were randomly divided into 3 groups, 10 in each group. After fasting for 16 hours, the blood lipid-lowering drug 2g / kg (that is, 0.1ml / 10g) prepared by intragastric administration was given to the administration group, and 0.1ml / distilled water was fed to the normal group and the high-fat control group. One hour after the administration, except for t...

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Abstract

The invention provides a nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide and application thereof and belongs to the technical field of antihyperlipidemic oligopeptides. Thenibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide is a peptide composed of amino acid sequences shown as SEQ ID NO: 1, and the ESI-MS (electrospray ionization mass spectrometry) measured molecular weight of the nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide is 687.75 Da. The invention relates to application of the nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide to preparing antihyperlipidemic drugs and / or healthcare products and / or food additives. The nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide can reduce the expression level of lipogenic genes, promote beta-oxidation of fatty acids, reduce lipid generation and achieve high lipid-lowering activity. The nibea albiflora swimming bladder-originated antihyperlipidemic hexapeptide can be applied to developing antihyperlipidemic drugs, healthcare products and food additives.

Description

technical field [0001] The invention belongs to the technical field of blood lipid-lowering oligopeptides, in particular to a blood-lipid-lowering hexapeptide derived from the swim bladder of yellow croaker and its application. Background technique [0002] Sea fish swim bladder is one of the "Eight Treasures of the Ocean" and is known as "Ocean Ginseng". The main components of fish maw are high-grade collagen, mucopolysaccharides, and contain various vitamins, calcium, zinc, iron, selenium and other trace elements. Swim bladder can be well digested and utilized by the human body because of its low fat content and its main component is collagen protein, a biological macromolecule. Like most meats, it is the raw material for human body to supplement and synthesize protein. Collagen in the human body is hidden in tissues and organs in the form of aqueous solution, which plays a role in improving the nutritional status of tissues. At the same time, collagen protein can also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61K38/08A61P3/06A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P3/06C07K7/06C12P21/06A23V2200/3262A23V2250/55
Inventor 王斌赵文浩孙坤来
Owner 中科利君股份有限公司
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