RH (Rhein) liposome nanoparticles with kidney targeted distribution characteristics and application

A technology of distribution characteristics and lipid vesicles, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of not being able to effectively improve the efficacy of drugs, and achieve the improvement of multiple pathologies indicators, promote clinical transformation, and improve the effect of therapeutic effects

Active Publication Date: 2019-03-08
浙江省中医院、浙江中医药大学附属第一医院
View PDF12 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the nanoparticles intercepted by the kidneys cannot be quickly absorbed and endocytized by the kidne...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RH (Rhein) liposome nanoparticles with kidney targeted distribution characteristics and application
  • RH (Rhein) liposome nanoparticles with kidney targeted distribution characteristics and application
  • RH (Rhein) liposome nanoparticles with kidney targeted distribution characteristics and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1 Materials and Instruments

[0031] Kidney Targeting Peptide KCSAVPLC (Kidney Targeting Peptide, KTP, entrusted to Shanghai Qiangyao Biotechnology Co., Ltd. to synthesize, purity ≥ 95%); RH API (Nanjing Zelang Pharmaceutical Technology Co., Ltd., purity ≥ 98%, batch number 201307); RH Reference substance (National Institute for Food and Drug Control, batch number 110757200206); Ethanolamine-polyethylene glycol 2000 (DSPE-PEG) (Shanghai Evert Pharmaceutical Technology Co., Ltd.); 1,2-distearoyl-sn-glycerol-3-phosphoethanolamine-polyethylene glycol 2000-N- Hydroxysuccinimide (DSPE-PEG-NHS) (Nanosoft Polymers, USA); 2-methyl-2-oxazoline, methyl p-toluenesulfonate, 1-3′-dimethylaminopropyl-3 -Ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) (Anaiji Chemical-Saen Chemical Technology Co., Ltd.); carboxyl-terminated polycaprolactone (PCL, molecular weight 3.7kD , Jinan Daigang Bioengineering Co., Ltd.); Thiazole blue (MTT) (Shanghai Aladdin Reagent Co., Ltd...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to RH (rhein) liposome nanoparticles with kidney targeted distribution characteristics and an application of the RH liposome nanoparticles. The lipid capsule nanoparticles have the characteristics of higher encapsulation efficiency and drug loading capacity for RH, uniform particle size distribution, slow release with adoption of in-vitro release, rapid cell uptake, avoidanceof entering lysosome and good biological safety. In a streptozotocin-induced diabetic nephropathy mouse model, KLPPR (Kidney targeting peptide modified Liposomal PCL-PEI nanoparticle loading Rhein) has the good kidney targeted distribution characteristics, can significantly improve a plurality of pathological indexes of the diabetic nephropathy mouse and alleviate and reverse deterioration of thecourse of diabetic nephropathy, and effectively improves the therapeutic effect of RH on diabetic nephropathy. According to the nanoparticles, a brand-new strategy is provided for targeted treatmentof diabetic nephropathy, clinical transformation of RH drugs is further promoted, and an important reference is provided for research of novel nano-preparations of RH and similar drugs.

Description

(1) Technical field [0001] The invention relates to a rhein lipid vesicle nanoparticle with kidney-targeted distribution characteristics and an application thereof. (2) Background technology [0002] Rhein (RH) is an active ingredient isolated and extracted from traditional Chinese medicines such as Polygonaceae plants Rheum palmatum L., Tanguticum Maxim.ex Balf., and belongs to the monoanthracene nucleus1, 8-dihydroxyanthraquinone derivatives, pharmacological studies have confirmed that RH can significantly inhibit the proliferation, hypertrophy and extracellular matrix production of glomerular mesangial cells, and RH can inhibit TSP-1 and TGF-β1 of human renal tubular epithelial cells The mRNA transcription and protein expression of the protein protect the inherent function of the kidney, and play a multi-target and multi-level therapeutic role in the intervention of kidney diseases. However, RH has poor solubility, short half-life, low renal enrichment rate, low bioavail...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/51A61K47/34A61K47/42A61K31/192A61P13/12A61P3/10
CPCA61K9/5146A61K9/5169A61K31/192A61P3/10A61P13/12
Inventor 陈丹飞朱永琴罗静雯魏颖慧施晓伟盛丽先王国伟
Owner 浙江省中医院、浙江中医药大学附属第一医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap