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Lymantria dispar Linnaeus JHAMT gene, encoded protein thereof and application of dsRNA of Lymantria dispar Linnaeus JHAMT gene in pest control

A protein-encoding and pest control technology, applied in the field of molecular biology, can solve the problems of gypsy moth resistance and pollution of the environment

Active Publication Date: 2019-03-12
HEZE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of drug resistance and environmental pollution in the existing chemical control of gypsy moth, and provides a kind of application of gypsy moth JHAMT gene, its encoded protein and its dsRNA in pest control

Method used

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  • Lymantria dispar Linnaeus JHAMT gene, encoded protein thereof and application of dsRNA of Lymantria dispar Linnaeus JHAMT gene in pest control
  • Lymantria dispar Linnaeus JHAMT gene, encoded protein thereof and application of dsRNA of Lymantria dispar Linnaeus JHAMT gene in pest control
  • Lymantria dispar Linnaeus JHAMT gene, encoded protein thereof and application of dsRNA of Lymantria dispar Linnaeus JHAMT gene in pest control

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specific Embodiment approach 1

[0020] Embodiment 1: The nucleotide sequence of the JHAMT gene of the gypsy moth in this embodiment is shown in SEQ ID NO: 1 in the sequence listing. The nucleotide sequence of its coding region CDS is shown in SEQ ID NO: 2 in the sequence listing.

specific Embodiment approach 2

[0021] Embodiment 2: The amino acid sequence of the protein encoded by the coding region CDS of the gypsy moth JHAMT gene in this embodiment is shown in SEQ ID NO: 3 in the sequence listing.

specific Embodiment approach 3

[0022] Embodiment 3: The dsRNA sequence of the JHAMT gene of the gypsy moth in this embodiment is shown in SEQ ID NO: 4 in the sequence listing.

[0023] The present invention uses the gypsy moth JHAMT gene fragment as a template, and designs a specific dsRNA primer pair, and synthesizes the JHAMT gene dsRNA through a MEGAscript RNAi kit (Ambion).

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Abstract

The invention discloses a Lymantria dispar Linnaeus JHAMT gene, encoded protein thereof and application of dsRNA of the Lymantria dispar Linnaeus JHAMT gene in pest control, relates to the field of molecular biology, and particularly relates to the Lymantria dispar Linnaeus JHAMT gene, the encoded protein and the application of the Lymantria dispar Linnaeus JHAMT gene. The invention aims to solvethe problems of drug resistance and environmental pollution in existing chemical control of the Lymantria dispar Linnaeus. The nucleotide sequence of the Lymantria dispar Linnaeus JHAMT gene is as shown in SEQ ID NO: 1 in a sequence table, and the amino acid sequence of a coding region CDS encoded protein is shown in SEQ ID NO: 3 in the sequence table. The dsRNA of the JHAMT gene causes Lymantriadispar Linnaeus larvae to prolong the 3rd instar of ecdysis, meanwhile the fresh weight increases slowly, and the cumulative mortality rate reaches 80.00%, seriously affecting the normal growth and development of the Lymantria dispar Linnaeus larvae. The invention is used in the field of Lymantria dispar Linnaeus pest control.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to the gypsy moth JHAMT gene, its encoded protein and its application. Background technique [0002] The gypsy moth (Lymantria dispar Linnaeus) is an important agricultural and forestry leaf-eating pest. It is widely distributed in my country and can harm more than 500 species of plants such as poplar, willow, apple, sylvestris pine, and larch. loss. At present, chemical control still occupies the main position in the control of gypsy moth pests, and biological control and physical control are usually only used as auxiliary control methods. The long-term, high-frequency, and continuous increase in the use of chemical pesticides has led to insect resistance, rampant growth, and environmental pollution such as pesticide residues. The emergence of disadvantages after the use of chemical pesticides has promoted the development of a new generation of pest control research. The rapid de...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/113C07K14/435A01N57/16A01P7/04
CPCA01N57/16C07K14/43563C12N15/113C12N2310/14
Inventor 问荣荣王步勇王娟孟欣慧
Owner HEZE UNIV
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