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Biological marker human serum C4BP-alpha (C4 binding protein-alpha) for predicting resistance of clopidogrel and clinical application thereof

A clopidogrel resistance, human serum technology, applied in material resistance, biological testing, biological material analysis, etc., can solve the problems of unclear mechanism and lack of clopidogrel resistance biomarkers, etc.

Active Publication Date: 2019-03-12
普灵生物(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reasons for patients to develop clopidogrel resistance, but the mechanism is not very clear, and there is a lack of clinically effective biomarkers for predicting clopidogrel resistance

Method used

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  • Biological marker human serum C4BP-alpha (C4 binding protein-alpha) for predicting resistance of clopidogrel and clinical application thereof
  • Biological marker human serum C4BP-alpha (C4 binding protein-alpha) for predicting resistance of clopidogrel and clinical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] Application of human serum C4BP-a protein detection in the diagnosis of clopidogrel resistance

[0017] 1. A kit for detecting C4BP-a (protein) levels in human serum, comprising the following components:

[0018] Deionized water

[0019] PBS (1×)

[0020] 96-well plate (pre-coated)

[0021] Orifice plate film

[0022] Standard

[0023] C4BP-a Standard Diluent

[0024] Detection solution A

[0025] Assay diluent A

[0026] Detection solution B

[0027] Assay diluent B

[0028] TMB substrate solution

[0029] stop solution

[0030] Concentrated washing liquid (30×)

[0031] 2. How to use the kit

[0032] The C4BP-a content in the patient's serum was detected with a commercially available ELISA kit (product of Wuhan Cloud Clone Technology Co., Ltd.). The specific operation is as follows.

[0033] 1. Preparation of C4BP-a standard curve (prepared within 15 minutes before use):

[0034] 1) Add 1mL of C4BP-a standard diluent to each bottle of standard product, ...

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Abstract

The invention discloses a biological marker for predicting resistance of clopidogrel and clinical application thereof, and relates to application of human serum C4BP-alpha (C4 binding protein-alpha) as a target or a standard product in preparation of kits for predicting resistance of clopidogrel. The kit for predicting the resistance of clopidogrel comprises an antibody for detecting human serum C4BP-alpha and other related reagents. In the clinical application, the resistance of clopidogrel can be predicted by the detection of human serum C4BP-alpha, and a fully novel detection method for instructing the reasonable utilization of medicine and assisting doctors to diagnose or predict the clinical treatment effect and prognosis of the clopidogrel is provided.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to human serum C4BP-a protein as a biomarker for predicting clopidogrel resistance and its clinical application. Background technique [0002] As an antiplatelet drug that has been included in the WHO Essential Medicines List, clopidogrel is used for acute coronary syndrome (ACS) or percutaneous coronary intervention (PCI) patients with coronary heart disease after implantation of drug-coated stents. The most commonly used drugs to prevent the recurrence of myocardial ischemia or stent thrombosis, combined with aspirin, the "dual antiplatelet therapy" regimen is the "gold standard" antiplatelet therapy regimen after ACS or PCI implantation of stents, which is the recognized and adopted by the world. [0003] Clopidogrel resistance (or tolerance) refers to the phenomenon that patients cannot achieve the expected antiplatelet effect after taking the recommended (or standard) dose of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/535
CPCG01N33/535G01N33/558G01N33/49G01N33/566C12Q1/56C12Q1/6827G01N27/04G01N33/5008G01N2800/52
Inventor 谢红光吉金子黄贝贝邰婷米琼宇谢文剑
Owner 普灵生物(南京)有限公司