A kind of rapid propagation method of warm curcuma sterilized seedlings

Abstract

The present invention provides a rapid propagation technology of warm curcuma sterilized seedlings, comprising the following steps: ① acquisition of explants; ② sterilization of explants; ③ induced tissue culture seedlings with clustered buds; ④ induction of root tip callus; ⑤Induction of sterilized turmeric vaccine; ⑥Reproduction of bacterin-free turmeric. The present invention adopts root tip meristem of tissue-cultured seedlings to induce callus, and obtains sterilized seedlings through redifferentiation, which effectively solves the difficult problem of directly obtaining aseptic meristems from explants, and also ensures the safety of sterilized seedlings. Obtained and solved the problem that the tissue cultured seedlings induced by explants cannot be continuously multiplied due to carrying endophytes; this technology invented the 6-BA+0.5mg / L of MS+2.0mg / L The NAA medium is used for continuous multiplication and cultivation, and the simultaneous realization of multiplication and rooting is realized, which saves the process of rooting and strong seedlings, and saves the cost of tissue culture.

Description

technical field [0001] The invention relates to the technical field of warm turmeric propagation, in particular to a method for rapid propagation of warm curcuma sterilized seedlings. Background technique [0002] Wenyujin is a perennial herb of the Zingiberaceae Curcuma genus. It has the value of medicine, health care, food, beauty and garden ornamental. It is an important economic crop. The part of Wenyujin used as a traditional Chinese medicine is a tuber, which needs to be processed differently to form "Wenyujin", "Wenyujin", and "Piece Turmeric". It is often used to relieve qi stasis, calm pain, and fight inflammation. The volatile oil containing sesquiterpene alcohols and sesquiterpene compounds is extracted from the rhizome of turmeric. The ingredients in it can effectively treat tumors and reduce the course of thrombus. It is a very effective drug and has a very broad development prospect. At the same time, Wen Yujin is also often used to reduce the degree of platel...

AI Technical Summary

Problems solved by technology

[0004] The traditional production mode of long-term vegetative propagation of Wen Yujin is far from being able to meet the market's requirements for the quality and output of Wen Yujin, and cannot adapt to modern production. Therefore, in order to improve the production and quality of Wen Yujin, modern biological cultivation methods must be used. Such as tissue culture technology, in order to obtain the sterilized vaccine quickly and efficiently

At present, there is no detailed report on the sterilized and rapid propagation of yujin. Therefore, this experiment intends to use the sterilized seedlings of yujin to quickly produce yujin through the rapid propagation system of yujin. The implementation provides a theoretical basis, and at the same time provides an effective way for factory production of seedlings to solve the long-term interference with the economic reduction of turmeric growers due to seed retention

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