Sulfhydrylation calcium alginate microspheres as intestinal drug delivery carrier and preparation method of sulfhydrylation calcium alginate microsphere
A kind of drug delivery carrier, the technology of calcium alginate
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Embodiment 1
[0021] Prepare a sodium alginate aqueous solution with a mass concentration of 1%, add 5mL of a 30mM carbodiimide aqueous solution and stir for 45min, then add 1g of L-cysteine and mix well, and adjust the pH of the above mixed solution to 5. Stir and react in the dark at room temperature for 3 hours, dialyze twice in 8000~14000kDa dialysis bags with 1mM hydrochloric acid solution as the outer fluid for dialysis, then dialyze twice with 1mM hydrochloric acid solution containing 1% sodium chloride as the outer fluid for dialysis, and finally Dialyze twice with 1mM hydrochloric acid solution as the dialysis external fluid, each dialysis time is 12~24h, and the reaction solution obtained after dialysis is freeze-dried to prepare the L-cysteine-sodium alginate conjugate; the sodium alginate and the L-cysteine-sodium alginate conjugate with a sulfhydryl content of 400 μmol / g were uniformly mixed at a mass ratio of 3:1, and then prepared into a mixed aqueous solution with a mass co...
Embodiment 2
[0023] Dissolve 7g of sodium alginate in 50mL of water, then add 3g of thioglycolic acid and 2mL of 7M hydrochloric acid solution, stir and react at 80°C for 2.5h in the dark, then pour the resulting reaction mixture into 250mL of ethanol, filter and dissolve The precipitate was washed twice with ethanol, and dried at room temperature to prepare the thioglycolic acid-sodium alginate conjugate. Sodium alginate and thioglycolic acid-sodium alginate conjugate with a mercapto group content of 300 μmol / g were uniformly mixed at a mass ratio of 2:1, and then prepared into a mixed aqueous solution with a mass concentration of 1% as a dispersed phase; peanut oil and Petroleum ether is mixed at a volume ratio of 10:6, and then propylene glycol monostearate accounting for 3% of the mass of the mixed oil phase is added as the continuous phase; 1 volume part of the dispersed phase is added dropwise to 13 volume parts of the continuous phase, and the Emulsify at 300rpm for 45min, then add ...
Embodiment 3
[0025] Prepare a sodium alginate aqueous solution with a mass concentration of 3%, add 5mL of a 45mM carbodiimide aqueous solution and stir for 45min, then add 3g of N-acetylcysteine and mix well, and adjust the pH of the above mixed solution with 1M hydrochloric acid solution To 5, stir and react at room temperature for 3 hours, then dialyze twice in 8000~14000kDa dialysis bags with 1mM hydrochloric acid solution as the external fluid for dialysis, and then dialyze twice with 1mM hydrochloric acid solution containing 1% sodium chloride as the external fluid for dialysis. Finally, 1mM hydrochloric acid solution was used as the dialysis external fluid for dialysis twice, each dialysis time was 12-24h, and the reaction solution obtained after dialysis was freeze-dried to prepare N-acetylcysteine-sodium alginate conjugate; seaweed Sodium acid and N-acetylcysteine-sodium alginate conjugated with a thiol content of 267 μmol / g were uniformly mixed at a mass ratio of 4:1, and then p...
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