Supercharge Your Innovation With Domain-Expert AI Agents!

MiR-4753-5p for diagnosing osteoporosis of postmenopausal women

An osteoporosis, women's technology, applied in the field of biomedicine, can solve the problem of not being able to use the screening of osteoporosis patients

Inactive Publication Date: 2019-03-19
王冉东
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The objects of auxiliary examination are generally patients with advanced osteoporosis, and cannot be used for the screening of patients with early osteoporosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MiR-4753-5p for diagnosing osteoporosis of postmenopausal women
  • MiR-4753-5p for diagnosing osteoporosis of postmenopausal women
  • MiR-4753-5p for diagnosing osteoporosis of postmenopausal women

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Sequencing and screening of miRNAs related to osteoporosis in postmenopausal women

[0048] 1. Research objects and sample collection

[0049] 3 cases of postmenopausal women with osteoporosis and 3 normal postmenopausal women, normal postmenopausal women served as controls. The clinical information is shown in Table 1.

[0050] Table 1 Clinical information of study subjects

[0051]

[0052] The subjects were required to fast for at least 12 hours. At room temperature from 7:00 to 8:00 the next morning, 10ml of venous blood was drawn into an ethylenediaminetetraacetic acid (EDTA) anticoagulant tube, and peripheral blood mononuclear cell PBMCs were extracted, and 1ml Trizol reagent was added. (Invitrogen), mix well and store the specimen at -80°C for RNA extraction. All blood samples and pathological results should be true and reliable, the study was approved by the ethics committee, and the patient informed consent.

[0053] 2. RNA extraction from blood monocytes

[...

Embodiment 2

[0080] Example 2 QPCR verification of differentially expressed miR-4753-5p

[0081] 1. According to the sequencing results in Example 1, miR-4753-5p was selected for large sample QPCR verification. According to the sample collection method in Example 1, 30 normal postmenopausal women and 30 postmenopausal women with osteoporosis were selected for separation and preservation of blood mononuclear cells.

[0082] 2. The RNA extraction process is the same as in Example 1.

[0083] 3. Reverse transcription: mix 10pg-1μg of total RNA template with 2μl 10* buffer, 2μl dATP (10mM), 0.5μl polyA polymerase, 0.5μl ribonuclease (RNase) inhibitor and RNase free water ) Mix, the final volume is 20μl, and incubate at 37°C for 1h. Then add 1μl 0.5μg / μl Oligo(dT) specific RT primer to the reaction tube, incubate at 70℃ for 5min and immediately incubate on ice for at least 2min to break the secondary structure of RNA and primer. Finally, the above 20μl reaction mixture and 4μl 5* buffer, 1μl dNTP (...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of miR-4753-5p in diagnosing the osteoporosis of postmenopausal women. The content of the miR-4753-5p in blood of patients with osteoporosis in the postmenopausal women is obviously different from that of the normal postmenopausal women, and the patients with the osteoporosis in the postmenopausal women and the patients with osteoporosis in the non-postmenopausal women can be distinguished according to the difference. The invention also discloses a diagnosis product for the osteoporosis of the postmenopausal women; the diagnosis product can realize the diagnosis purpose of the osteoporosis of the postmenopausal women by detecting the miR-4753-5p in the blood.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of miR-4753-5p in the early diagnosis of osteoporosis in postmenopausal women. Background technique [0002] miRNA is a type of single-stranded small RNA, which is reverse complementary to the mRNA transcripts of other protein-coding genes, and the length is generally 18-25 nt. It is produced by DNA transcription, but is not translated into protein, but regulates the expression and function of its target gene during protein synthesis. Therefore miRNA is a gene that regulates other protein-coding genes. Since miRNAs play an important role in the regulation of gene expression, differences in miRNA expression can cause a variety of human diseases. Currently known disease systems related to miRNA expression disorders include: tumors, rheumatic diseases, metabolic diseases, cardiovascular diseases, and Nervous system diseases and so on. [0003] Osteoporosis (OP) is a group of bon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 王冉东
Owner 王冉东
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com