Application of metal-nitrogen doped porous carbon materials in mass spectrometry detection

A nitrogen-doped porous carbon and metal technology is applied in the field of mass spectrometry detection, which can solve the problems that small molecular substances cannot obtain molecular weight signals well, difficult to analyze volatile molecules, and difficult to obtain signals, etc., and achieves simple and efficient mass spectrometry detection capabilities. , Improve the detection ability of mass spectrometry, the effect of broad application value

Active Publication Date: 2020-09-29
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MALDI mass spectrometry, as a commonly used method for analyzing the molecular weight of analytes, is widely used in various detection and analysis. The main problems currently faced are: 1. Small molecular substances cannot obtain their molecular weight signals well due to matrix interference; 2. Since the sample is in a vacuum when testing the sample, it is difficult to obtain signals for substances with high vapor pressure and volatile
Among them, the construction of micro-nano structures is time-consuming and costly. However, when using inorganic materials as substrates to analyze samples, it is usually difficult to solve the problem of analyzing volatile molecules in vacuum due to the limited binding ability of materials and molecules to be measured.

Method used

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  • Application of metal-nitrogen doped porous carbon materials in mass spectrometry detection
  • Application of metal-nitrogen doped porous carbon materials in mass spectrometry detection
  • Application of metal-nitrogen doped porous carbon materials in mass spectrometry detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1, using Co-NC as a substrate to detect thymopentin

[0049] Matrix dispersion: Weigh 5mg of Co-NC powder and put it in 1mL of ethanol, ultrasonically disperse for 20min. Take 1 μL and spot it on the stainless steel target surface, and let it dry at room temperature.

[0050] The preparation concentration is 10 -3 Take 1 μL of the thymopentin solution of M and put it on the dried matrix spot, and let it dry at room temperature for testing.

[0051] Test results such as figure 2 As shown, it can be seen that using Co-NC as a matrix can effectively ionize thymopentin molecules and obtain a spectrum with a good signal-to-noise ratio. And it can be seen in the figure that there is little interference in the small molecule region.

Embodiment 2

[0052]Example 2, using Co-NC as a substrate for enrichment and a detection concentration of 10 -4 M glucose

[0053] Prepare 10 -4 M glucose solution, take 5 mg of Co-NC powder and put it in the prepared 1 mL glucose solution, after ultrasonication for 20 min, take 1 μL spot on the stainless steel target surface, and test after drying at room temperature.

[0054] Test results such as image 3 As shown, it can be seen that after using Co-NC solid to enrich glucose molecules, a spectrum with a good signal-to-noise ratio can be obtained.

[0055] Using conventional DHB as the substrate for a concentration of 10 -4 M glucose samples were tested by mass spectrometry, and the detection results were as follows Figure 4 As shown, it can be seen that the interference is obvious.

Embodiment 3

[0056] Example 3, using Co-NC as a substrate to detect the high-resolution mass spectrum of an alkylthiophene sample

[0057] The preparation method of the matrix dispersion refers to Example 1, and then take 1 μL of the alkylthiophene (formula I) sample and spot it on the dried matrix spot, and enter the instrument for testing.

[0058]

[0059] Test results such as Figure 5 As shown, it can be seen that the molecular weight of the alkylthiophene sample can be obtained using Co-NC as a matrix with a good signal-to-noise ratio. However, the analysis of alkylthiophene with the traditional matrix CHCA cannot obtain the signal of the target molecule. This is due to the high vapor pressure of alkylthiophene, which will quickly volatilize in the vacuum of the mass spectrometer, making it impossible to test using traditional methods. The Co-NC matrix has a porous structure, and due to the large π system, it can effectively adsorb the alkylthiophene, thus protecting the sample ...

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Abstract

The invention discloses application of a metal-nitrogen doped porous carbon material in mass spectrometry detection. The metal-nitrogen doped porous carbon material is obtained by pyrolyzing a precursor in inert atmosphere, the precursor is a mixture of metal salt and carbon-nitrogen-containing organic matter and / or nitrogen-containing organic matter, and the metal salt can be iron salt, cobalt salt or nickel salt. The testing range of MALDI mass spectrometry is expanded, volatile samples high in vapor pressure can be detected; the metal-nitrogen doped porous carbon material is mild, simple and convenient in synthesis condition, nontoxic, harmless, simple in operation during MALDI testing and reusable and has quite extensive application value in the field of analysis detection. A method which is simple, convenient, efficient and capable of improving mass spectrometry detection ability is provided and can be further used for bioanalysis and MALDI mass spectrometry imaging.

Description

technical field [0001] The invention relates to the application of a metal-nitrogen-doped porous carbon material, in particular to the application of the metal-nitrogen-doped porous carbon material in mass spectrometry detection, and belongs to the technical field of mass spectrometry detection. Background technique [0002] Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry (MALDI mass spectrometry) is a new type of soft ionization mass spectrometry developed in recent years. MALDI mass spectrometry, as a commonly used method for analyzing the molecular weight of analytes, is widely used in various detection and analysis. The main problems currently faced are: 1. Small molecular substances cannot obtain their molecular weight signals well due to matrix interference; 2. Since the sample is in a vacuum when the sample is tested, it is difficult to obtain a signal for a substance with a high vapor pressure and is volatile. [0003] At present, in order to solve the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 赵镇文李书沐刘健安张阳阳
Owner INST OF CHEM CHINESE ACAD OF SCI
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